Tauto_00210 Tauto_00210   Tauto_00230 Tauto_00230

Tauto_00220 : CDS information

close this sectionLocation

Organism
Strain
Entry nameTautomycin
Contig
Start / Stop / Direction88,025 / 86,802 / - [in whole cluster]
88,025 / 86,802 / - [in contig]
Locationcomplement(86802..88025) [in whole cluster]
complement(86802..88025) [in contig]
TypeCDS
Length1,224 bp (407 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative CoA transferase
Product (GenBank)predicted acyl-CoA transferases/carnitine dehydratase
Gene
Gene (GenBank)ttmP
EC number
Keyword
  • dialkylmaleic anhydride moiety
Note
Note (GenBank)
Reference
ACC
PmId
[18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008)
comment
tautomycin (TTM) biosynthetic gene clusterについての文献。

ttmP(407a.a.): CoA transferase

ttmPを不活化すると、TTMの生産が見られないことを確認している。機能としては、typeIII CoA transferase familyに属してgamma-butyrobetaine-CoA とcarnitine間でCoA moietyを転移してcarnityl-CoAとgamma-butyrobetaineを生じるCaiBによく似ていることより、C4' carboxylic acidを含む閉環に関与することが予想されている。
Related Reference
ACC
Q1KLK0
PmId
[16547052] Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus. (J Bacteriol. , 2006)
comment
<Succinyl-CoA:L-malate CoA transferase subunit B, Chloroflexus aurantiacus, BLAST 158; id=41%>

Chloroflexus aurantiacusよりsuccinyl-CoA:L-malate coenzyme A transferaseを精製し、その二つのサブユニット(subunit A, B)のN末端アミノ酸配列を決定し、遺伝子を同定している。また、クローニングした二つの遺伝子をE. coliで発現させて、諸性質を調べている。
ACC
Q1KLK1
PmId
[16547052] Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus. (J Bacteriol. , 2006)
comment
<Succinyl-CoA:L-malate CoA transferase subunit A, Chloroflexus aurantiacus, BLAST 185; id=41%>

Chloroflexus aurantiacusよりsuccinyl-CoA:L-malate coenzyme A transferaseを精製し、その二つのサブユニット(subunit A, B)のN末端アミノ酸配列を決定し、遺伝子を同定している。また、クローニングした二つの遺伝子を E. coliで発現させて、諸性質を調べている。

close this sectionSequence

selected fasta
>putative CoA transferase [predicted acyl-CoA transferases/carnitine dehydratase]
MAIPRGRTECGRPALPALAAPLAGLTVIDASTLFAGPFAARLLGDFGAEIIKVEQPSGDP
LRRFGHAHDDVPLLWKVLSRNKKSVVLDLHEPEDAQRFRQLAAGADALIENFRPGTLERW
GLGPEVLAEANPRLVLARVTAFGQDGPYARRPGFGTLAEAMSGLAAMSGEPDGPPALPAF
PLGDAIAGLHAALAVLIALRARDVIGTGQIADIAITETLIGSLGAQLTVYEHLGLKPERI
GNRSNNSAPRGVYRCADDRWVAVSAPARSVAERVVRLVGRPELGAEPWFATGVGRAEHRE
LIDDAIRPWIAARCRDEVVDAFEAAEAAVAPIYEVDDVLADPQFRARGLTVDVPDAELGS
VRMPGVPFRLSATPGRIDWAGPPIGAHTEDVCGSVAPARSTAGEAGA
selected fasta
>putative CoA transferase [predicted acyl-CoA transferases/carnitine dehydratase]
ATGGCGATTCCGCGCGGCAGAACCGAATGTGGCAGGCCGGCCCTGCCCGCTCTCGCGGCA
CCTTTGGCCGGGCTCACCGTGATCGACGCCTCCACGCTGTTCGCGGGACCGTTCGCGGCG
AGACTGCTCGGGGACTTCGGCGCCGAGATCATCAAGGTGGAACAGCCCTCGGGTGATCCG
CTGCGCCGCTTCGGCCATGCGCACGACGACGTCCCGCTGCTGTGGAAGGTGCTGAGCCGC
AACAAGAAGAGCGTTGTGCTGGATCTGCACGAGCCTGAGGACGCGCAGCGGTTCCGCCAG
CTCGCCGCCGGGGCCGACGCCCTCATCGAGAACTTCAGGCCGGGCACCCTGGAACGCTGG
GGCCTCGGACCGGAGGTGCTCGCAGAGGCCAACCCTCGGCTGGTGCTGGCCAGGGTCACC
GCCTTCGGACAGGACGGCCCGTACGCACGCCGCCCCGGCTTCGGCACGTTGGCGGAAGCC
ATGAGCGGGCTGGCGGCGATGTCGGGTGAACCCGACGGGCCGCCCGCGCTCCCGGCGTTC
CCGCTCGGCGACGCCATCGCCGGCCTGCACGCGGCTCTCGCGGTGCTGATCGCGCTGCGC
GCCCGCGACGTGATCGGCACCGGCCAGATCGCGGACATTGCCATCACCGAGACGCTCATC
GGCTCACTGGGAGCCCAGCTCACCGTGTACGAACACCTCGGCCTCAAGCCCGAACGGATC
GGCAACCGGTCCAACAACAGCGCGCCTCGGGGCGTCTACCGTTGCGCGGACGACCGCTGG
GTCGCCGTCTCCGCCCCGGCGCGCTCGGTCGCCGAACGCGTCGTCCGGCTGGTCGGCCGC
CCCGAGCTGGGCGCTGAGCCGTGGTTCGCCACCGGGGTCGGTCGGGCCGAGCACCGCGAG
CTGATCGACGACGCGATCCGGCCGTGGATCGCAGCCCGGTGCCGGGACGAGGTCGTGGAC
GCCTTCGAGGCGGCCGAAGCGGCCGTCGCCCCCATCTACGAGGTCGACGACGTGCTGGCC
GATCCGCAGTTCCGGGCGCGCGGTCTCACCGTGGACGTGCCCGACGCCGAGCTCGGCTCG
GTGCGCATGCCCGGGGTCCCGTTCCGGCTGTCGGCCACTCCGGGCCGGATCGACTGGGCC
GGTCCGCCGATCGGCGCCCACACCGAGGACGTGTGCGGCTCCGTCGCGCCCGCCCGCTCG
ACGGCCGGGGAGGCCGGGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003673 CoA-transferase family III (Family)
[85-267] 1.99999999999999e-60 PF02515
PF02515   CoA_transf_3
IPR023606 CoA-transferase family III domain (Domain)
[20-331] 2.39999999999997e-99 G3DSA:3.40.50.10540
G3DSA:3.40.50.10540   CoA-Trfase_fam_III
[18-395] 3.30001976117415e-120 SSF89796
SSF89796   CoA-Trfase_fam_III
SignalP
[1-30] 0.938 Signal
Eukaryota   
[1-35] 0.706 Signal
Bacteria, Gram-positive   
[1-35] 0.332 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Tauto_00210 Tauto_00210   Tauto_00230 Tauto_00230
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