Tetrn_00030 Tetrn_00030   Tetrn_00050 Tetrn_00050

Tetrn_00040 : CDS information

close this sectionLocation

Organism
StrainNRRL 11266
Entry nameTetronomycin
Contig
Start / Stop / Direction5,425 / 6,216 / + [in whole cluster]
5,425 / 6,216 / + [in contig]
Location5425..6216 [in whole cluster]
5425..6216 [in contig]
TypeCDS
Length792 bp (263 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative type II thioesterase
Product (GenBank)putative type II thioesterase
Gene
Gene (GenBank)tmn6
EC number
Keyword
  • type II thioesterase
Note
Note (GenBank)
Reference
ACC
PmId
[18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008)
comment
Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。

tmn6(263 aa): type II thioesterase

tmn6の不活化変異株ではTMN生産が野生株の2-3%に低下することが示されている。
Related Reference
ACC
Q7BUF9
NITE
Rifam_00540
PmId
[19103602] Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. (J Biol Chem. , 2009)
comment
Blast 11th, id52%, 2e-62
Amycolatopsis mediterranei_rifR
RifR
[Rifam_00540]type II thioesterase

RifRは、acyl-CoAsよりもacyl carrier domainのphosphopantetheinyl armからのacyl unitsを加水分解する(Steady-state kinetics)。
helical lidの運動が、RifR active siteへの基質のアクセスを調節する(立体構造解析)。

RifRがCoA基質よりACP基質への反応を好むことは、type II thioesteraseがcarrier domainsから異常unitsを除くようなhousekeeping functionsをすると考えられていることに一致する。
ACC
Q6W5Q5
NITE
FR008_00110
PmId
[18836004] Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. (Appl Environ Microbiol. , 2008)
comment
Blast 38th, id48%, 6e-56
Streptomyces sp. FR-008_fscTE
FscTE
[FR008_00110]type II thioesterase

fscTEはputative type II thioesterase (TEII)をコードする。
fscTE deletionでFR-008/candicidin産生は約90%減。
FscTEは成熟伸長したpolyketideの放出を代償することができなかった。

FscTE and TylOのHydrolytic activitiesは、
propionyl-S-N-acetylcysteamine > methylmalonyl-S-N-acetylcysteamine
acetyl-S-N-acetylcysteamine > malonyl-S-N-acetylcysteamine

したがって、TEII はacyl carrier proteins(ACP)に結合するnon-elongatable residuesを選択的に除去することにより、効果的なpolyketide生合成を維持することが結論付けられる。

close this sectionSequence

selected fasta
>putative type II thioesterase [putative type II thioesterase]
MAPVPTQIPFDETWLRRFGTARQPDVRLVCLPHAGGAATTFFPLQRELPPSVEFVAVQYP
GRQDRRQEQPLRDVRTLADRVAAVLEPLTDLPIVLFGHSMGAVVGFETAARLEEKGANAP
IGLVASGRHSPATPRVENVHQRDDDGLIAEITTLSGTDPQILADPDMRAMILPALRADYT
AVETYEYDRGFVLRCPISVFTGTSDPRVTEHEAAAWESLTSGAFRIERFPGGHFYLEGAE
RAVATALMRDVRAFSATTTTPPR
selected fasta
>putative type II thioesterase [putative type II thioesterase]
ATGGCCCCGGTGCCCACACAGATACCGTTCGACGAGACGTGGCTGCGCCGCTTCGGCACG
GCCCGGCAGCCGGACGTGCGGCTTGTCTGTCTGCCCCACGCGGGCGGCGCGGCGACCACG
TTCTTCCCGCTGCAGCGTGAACTGCCGCCGTCCGTCGAGTTCGTGGCCGTGCAGTACCCG
GGCCGGCAGGACCGCCGCCAGGAACAGCCGCTGCGCGACGTCCGCACCCTCGCGGACCGC
GTGGCGGCCGTGCTCGAACCGCTGACCGATCTGCCGATCGTCCTCTTCGGGCACAGCATG
GGCGCGGTCGTCGGCTTCGAGACCGCAGCCCGGCTGGAGGAGAAGGGCGCCAACGCACCC
ATCGGCCTGGTCGCCTCGGGCCGGCACTCGCCGGCGACGCCGCGCGTGGAGAACGTGCAC
CAGCGCGATGACGACGGCCTCATCGCCGAGATCACCACGCTGAGCGGCACCGACCCGCAG
ATCCTGGCCGACCCGGACATGCGGGCCATGATCCTGCCGGCGCTGCGCGCCGACTACACC
GCCGTGGAGACGTACGAGTACGACCGCGGCTTCGTGCTGCGCTGCCCGATCAGCGTCTTC
ACGGGCACCTCGGACCCGCGGGTGACCGAGCACGAGGCGGCGGCCTGGGAGTCGCTGACC
TCCGGCGCGTTCCGCATCGAGCGGTTCCCCGGCGGCCACTTCTACCTCGAAGGTGCGGAG
CGGGCCGTCGCGACCGCCCTCATGCGCGACGTCCGCGCGTTCTCGGCCACGACCACGACG
CCGCCCCGTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[27-243] 5.79999999999997e-50 PF00975
PF00975   Thioesterase
SignalP No significant hit
TMHMM No significant hit
Tetrn_00030 Tetrn_00030   Tetrn_00050 Tetrn_00050
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