Tetrn_00100 Tetrn_00100   Tetrn_00120 Tetrn_00120

Tetrn_00110 : CDS information

close this sectionLocation

Organism
StrainNRRL 11266
Entry nameTetronomycin
Contig
Start / Stop / Direction30,048 / 25,324 / - [in whole cluster]
30,048 / 25,324 / - [in contig]
Locationcomplement(25324..30048) [in whole cluster]
complement(25324..30048) [in contig]
TypeCDS
Length4,725 bp (1,574 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)type I polyketide synthase
GenetmnAVI
Gene (GenBank)tmnVI
EC number
Keyword
Note
Note (GenBank)
  • module 12 (KS, AT-A, and ACP)
Reference
ACC
PmId
[18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008)
comment
Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。

tmnAVI(1,574 aa): PKS:
module 12 (KS, ATa, ACP)

tetronomycin (TMN) を合成するPKSをコードする。
module 12のKR domainは必須とされている活性残基が保存されていないためinactiveである。

close this sectionPKS/NRPS Module

12 malonyl-CoA
KS34..411
AT570..861
kr1197..1374
ACP1486..1556

close this sectionSequence

selected fasta
>polyketide synthase [type I polyketide synthase]
MSKEEKLVEYLKWVTAELHEAQQRLSVLETGDREPVAIVSMACRYPGGVRSPEDLWQLVA
DGSDAISGFPLNRGWELDGLFDADPDRSGTSYAREGGFLHDAAAFDAAFFEISPREATAT
DPQQRLLLEAAWETFERADLDPHSLRGSRTGVFAGVMYSDYVARLMDRLPPGFEGFLGNG
GAASVASGRVAYTFGLEGPAVTVDTACSSSLVSMHLAAQALRNEECDLALAGGVTVMATP
GVFVEFSRQRGLAPDGRCKPFAEAADGTGWGEGVGFVLLERLSDAERNGHEILAVLRGSA
VNQDGASNGLTAPNGPSQQRVIEQALASARLSAGQVDAVEAHGTGTTLGDPIEAQALLAT
YGQAHSAERPLWLGAVKSNIGHTQAAAGVAGVIKMVEAMRHGVLPRTLHVDEPSTHVDWN
TGHVALLTEAQQWPETGEPRRAAVSSFGISGTNAHVILEAAPEPGKPVEERVDKSMPVPV
VVSARSSAALAEAAGRLAAYVEHHPDVEIGALAARLWSGRAKLEHRAGVVTADPTELHEA
LTALAAGTTHPALVTGPGSAETGGRLAVMFSGQGSQRPGMGRDLYETFPAYAEALDEVCA
ALDQHLDTEVPLRDVMFAEPGSEHAALLETTLYTQPALFAHHVAGYRLLQKVGVQPAALI
GHSIGELSAAHLTGTLPLAVAADMVATRAKLLHTLPDGTGMLAVNTDADTLTPYLKRHED
VAVAAHNSATSLAVAGPHQPLRELAAELTEAGIRTKALKVAHAFHTAHTEPILDTFTGHL
TDLFNRHTLGDADIPVISNVTGLPATSGQHHDPEYWAQHIRQPVHFHQGITHLTTDDSIS
LFTELAPHPTLAPQLPSGTKRTPRLPTETLTHLATLVGLHGNHHPVDLAAHLPETAAKVP
ALPTYPFQHRPYWLRADPAKGTDARPGDAELWDALEREDLDGVLSAMGVRDADVTAWKAV
LPSLVTWRRQRGWGHRLGWEALDDGTPPLLDGAWVVLLPEDAAEHPAVRTALTALDEGGA
GVEVVTVERQMARTTFAGRLRAALSACGARLAGVLSLLAFADPARPGPDGLELSATLLHA
LDDAAAQVPVWFLTAGGTTAGGEDPLADPAQARIRGLAQSLAVEHPERWGGLADLPAGGA
GGRDVRDRLRAALAGRADRSGAREDQVAVRAGALFARRMRPADLAAATEPWLRATDGTVL
VTGAGGALAEEIAVQLVREGVRHLLLATGPGAYGEAAALVAQLREAGAEPVVAAIDLADR
DAVAALVRSVAPEHPLTAVVHVAAQLDDAVVGAVDADDVGRLLDPAAAALANLCELTLDR
GLESFVHCTSAVGALGAAGFGAQGAVHAHLEALAQTYRERGLPVTSVVCGPWQDPEAADT
AAGKHLRYQGIRPMAPRLAARLLVRAVAGRPATPVLVDVDWDRYVRQSEQGAAGSLLRAI
PEAGAGAGDGGAWEPETDGAGPLGEAAARLRQRLDTAAGAERAELLLELLRDAAAQVLGH
ADTGEIAADAQFTELGFSSFTALELCNRVAAVSGLEIPPMAIFDNPTPQALAGYLMTTFE
GETSLATAAGAAAR
selected fasta
>polyketide synthase [type I polyketide synthase]
ATGTCCAAGGAAGAGAAGCTCGTCGAGTACCTCAAGTGGGTCACCGCCGAGCTGCATGAG
GCCCAGCAGCGGCTGTCCGTCCTGGAGACCGGGGACCGGGAGCCGGTGGCGATCGTGTCG
ATGGCCTGCCGGTACCCGGGCGGCGTGCGGTCGCCCGAGGACCTGTGGCAGCTCGTCGCG
GACGGCTCGGACGCCATCTCCGGCTTCCCCCTGAACCGCGGCTGGGAGCTGGACGGACTC
TTCGACGCAGACCCCGACCGGTCCGGCACGAGCTACGCCCGCGAGGGCGGCTTCCTGCAC
GACGCGGCCGCCTTCGACGCGGCCTTCTTCGAGATCAGCCCGCGGGAGGCCACGGCCACG
GACCCGCAGCAGCGGCTGCTGCTGGAGGCCGCCTGGGAGACGTTCGAGCGCGCGGACCTG
GACCCGCACAGCTTGCGGGGCAGCAGGACCGGCGTCTTCGCGGGCGTCATGTACAGCGAC
TACGTAGCCCGGCTCATGGACCGGCTGCCGCCGGGGTTCGAAGGCTTCCTGGGCAATGGT
GGTGCGGCGAGTGTGGCGTCGGGTCGGGTGGCGTACACCTTCGGTCTCGAAGGCCCCGCG
GTCACCGTCGACACCGCCTGCTCCTCGTCGCTGGTGAGCATGCACCTGGCTGCGCAGGCG
TTGCGCAACGAGGAGTGTGATCTTGCGCTTGCCGGTGGTGTGACGGTGATGGCCACTCCG
GGTGTGTTCGTGGAGTTCTCGCGGCAGCGTGGGTTGGCGCCTGATGGGCGGTGCAAGCCG
TTCGCGGAGGCTGCCGACGGTACGGGCTGGGGCGAGGGTGTCGGTTTCGTGCTGCTGGAG
CGGCTGTCGGACGCGGAGCGCAACGGGCACGAGATTCTGGCGGTGCTGCGGGGTTCGGCC
GTCAACCAGGACGGCGCTTCCAACGGTCTGACCGCGCCCAACGGCCCTTCCCAGCAGCGG
GTCATCGAACAGGCGTTGGCCAGTGCGCGGTTGAGTGCGGGTCAGGTCGATGCGGTCGAG
GCGCACGGTACGGGTACGACGCTGGGCGACCCGATCGAGGCACAGGCGCTGCTGGCCACC
TATGGTCAGGCGCATTCTGCGGAGCGGCCGTTGTGGCTGGGTGCGGTGAAGTCGAACATC
GGTCACACCCAGGCCGCGGCGGGTGTCGCGGGTGTGATCAAGATGGTCGAGGCCATGCGC
CACGGCGTCCTGCCCCGCACCCTCCACGTCGACGAACCCTCCACCCACGTCGACTGGAAC
ACCGGCCACGTCGCCCTCCTGACCGAAGCGCAACAGTGGCCCGAGACGGGTGAGCCGCGA
CGCGCGGCGGTGTCGTCGTTCGGGATCAGCGGCACCAACGCGCACGTCATCCTGGAGGCC
GCCCCGGAGCCCGGTAAGCCGGTCGAGGAGCGGGTCGACAAGTCGATGCCGGTGCCGGTG
GTGGTCTCGGCGCGTTCGTCGGCGGCGCTGGCGGAGGCCGCGGGTCGGCTGGCGGCGTAC
GTCGAGCACCACCCGGACGTCGAGATCGGCGCGCTGGCGGCGCGACTGTGGTCCGGGCGC
GCGAAGCTGGAGCACCGCGCGGGCGTCGTCACCGCCGACCCCACCGAACTCCACGAAGCC
CTCACCGCACTCGCCGCCGGCACCACGCACCCGGCGCTGGTCACCGGCCCCGGGTCCGCC
GAGACCGGTGGGCGGCTTGCGGTGATGTTCTCCGGACAGGGCAGCCAACGCCCCGGCATG
GGCCGCGACCTGTACGAGACCTTCCCCGCCTACGCCGAAGCCCTGGACGAGGTCTGCGCC
GCGCTGGACCAGCACCTCGACACCGAGGTCCCACTACGGGACGTGATGTTCGCCGAACCC
GGCAGCGAGCACGCCGCGCTGCTGGAGACGACGCTCTACACCCAGCCCGCCCTCTTCGCC
CACCACGTGGCCGGCTACCGCCTCCTCCAAAAGGTAGGCGTTCAGCCCGCGGCGCTCATC
GGTCACTCCATCGGCGAACTCTCGGCCGCCCACCTCACCGGCACCCTCCCCCTCGCCGTG
GCCGCCGACATGGTCGCCACCCGCGCCAAGCTGCTCCACACCCTCCCCGACGGCACCGGC
ATGCTCGCCGTCAACACCGACGCCGACACCCTCACCCCGTACCTCAAGCGCCACGAAGAC
GTGGCCGTCGCCGCGCACAACTCCGCCACGTCCCTCGCCGTCGCCGGCCCGCACCAGCCG
CTCCGGGAACTCGCCGCGGAGCTGACCGAGGCGGGGATCCGCACCAAGGCTTTGAAGGTC
GCGCACGCCTTCCACACCGCGCACACCGAGCCGATCCTCGACACCTTCACCGGCCACCTG
ACGGACCTCTTCAACCGCCACACCCTCGGCGACGCCGACATCCCCGTCATCTCCAACGTC
ACCGGCCTACCCGCCACCAGCGGGCAACACCACGACCCCGAATACTGGGCCCAGCACATC
CGCCAACCCGTCCACTTCCACCAAGGCATCACCCACCTCACCACCGACGACAGCATCAGC
CTCTTCACCGAGCTGGCCCCGCATCCCACCCTCGCGCCCCAGCTCCCGTCCGGTACGAAG
CGGACCCCCCGTCTGCCGACGGAGACCCTCACCCACCTCGCCACCCTGGTCGGCCTCCAC
GGCAACCACCACCCCGTCGACCTCGCCGCCCACCTCCCCGAGACCGCGGCCAAGGTCCCC
GCGCTCCCCACCTACCCCTTCCAGCACCGCCCCTACTGGCTGCGCGCCGACCCCGCCAAG
GGGACCGATGCCCGCCCCGGCGACGCCGAATTGTGGGACGCACTGGAGCGCGAGGACCTG
GACGGCGTGCTGTCCGCGATGGGTGTGCGGGACGCCGATGTCACGGCGTGGAAGGCCGTC
CTGCCCTCGCTCGTCACCTGGCGCCGCCAGCGCGGCTGGGGCCACCGCCTCGGCTGGGAG
GCCCTGGACGACGGCACGCCGCCGCTGCTCGACGGCGCCTGGGTCGTCCTGCTCCCCGAG
GACGCCGCGGAGCACCCAGCCGTACGGACCGCGTTGACCGCGCTGGACGAGGGCGGAGCC
GGAGTCGAGGTCGTCACCGTCGAGCGGCAGATGGCCCGTACGACGTTCGCCGGCCGGTTG
CGTGCCGCGCTGAGCGCCTGCGGGGCCCGGCTCGCCGGAGTGCTGTCCCTGCTGGCGTTC
GCCGACCCGGCCCGGCCGGGCCCCGACGGGCTGGAGCTGTCCGCCACGCTGCTGCACGCG
CTCGACGACGCGGCCGCGCAGGTACCCGTGTGGTTCCTCACCGCGGGCGGTACCACGGCG
GGCGGCGAGGACCCGCTCGCCGACCCCGCCCAGGCCCGGATCCGGGGGCTGGCGCAGTCG
CTCGCCGTCGAACACCCCGAGCGCTGGGGCGGCTTGGCCGACCTGCCCGCCGGCGGTGCC
GGCGGCCGGGACGTACGCGACCGCCTCCGGGCGGCGCTCGCCGGGCGGGCCGACCGCTCG
GGCGCCCGCGAGGACCAGGTCGCCGTCCGGGCCGGGGCGCTGTTCGCCCGCCGGATGCGC
CCCGCGGACCTCGCCGCCGCCACCGAACCGTGGCTGCGCGCCACGGACGGCACCGTGCTG
GTGACCGGGGCGGGCGGCGCGCTCGCGGAGGAGATCGCCGTGCAGCTCGTACGCGAAGGC
GTACGGCACCTGCTGCTGGCCACCGGGCCCGGCGCGTACGGGGAAGCCGCAGCGCTGGTC
GCGCAGTTGCGGGAGGCGGGCGCGGAGCCCGTGGTGGCCGCCATCGACCTCGCCGACCGC
GACGCGGTCGCCGCACTCGTCCGCTCCGTCGCGCCCGAGCACCCGCTGACCGCCGTCGTG
CACGTCGCCGCCCAACTCGACGACGCCGTCGTCGGTGCGGTGGACGCCGACGACGTCGGC
CGGCTGCTCGACCCGGCCGCGGCGGCCTTGGCCAACCTCTGCGAGCTGACCCTCGACCGC
GGGCTGGAGTCGTTCGTCCACTGCACCTCCGCGGTCGGCGCCCTCGGCGCGGCCGGCTTC
GGCGCCCAGGGGGCGGTCCACGCCCACCTGGAGGCGCTGGCGCAGACGTATCGCGAACGC
GGCCTGCCCGTCACCTCCGTGGTCTGCGGCCCGTGGCAGGACCCGGAGGCGGCGGACACC
GCGGCCGGAAAGCACCTGCGCTACCAGGGCATCCGCCCCATGGCGCCGCGACTCGCGGCA
CGGCTGCTGGTACGCGCCGTCGCCGGCCGCCCGGCCACGCCCGTGCTGGTCGACGTCGAC
TGGGACCGCTACGTCAGGCAGTCCGAACAGGGCGCCGCCGGCTCCCTGCTGCGCGCGATC
CCCGAAGCCGGGGCCGGCGCGGGGGACGGCGGCGCCTGGGAGCCCGAGACCGACGGAGCC
GGGCCCCTCGGCGAGGCCGCGGCGCGGCTGCGCCAACGGCTCGACACCGCCGCCGGCGCC
GAGCGGGCCGAGCTGCTGCTGGAACTGCTGCGGGACGCCGCCGCCCAGGTCCTGGGACAC
GCGGACACCGGGGAGATCGCCGCCGACGCCCAGTTCACCGAGCTGGGGTTCTCCTCCTTC
ACCGCCCTCGAACTGTGCAACCGGGTCGCGGCCGTGTCGGGGCTGGAGATCCCGCCGATG
GCCATCTTCGACAACCCCACGCCGCAGGCGCTGGCGGGCTATCTGATGACCACCTTCGAG
GGGGAGACCTCCCTCGCCACAGCGGCCGGGGCCGCGGCCCGGTGA
[12] KS34..411
[12] AT570..861
[12] malonyl-CoA762..766
[12] kr1197..1374
[12] ACP1486..1556
[12] KS100..1233
[12] AT1708..2583
[12] malonyl-CoA2284..2298
[12] kr3589..4122
[12] ACP4456..4668

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[560-695] 1.40000000000001e-61 G3DSA:3.40.366.10 [762-855] 1.40000000000001e-61 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR009081 Acyl carrier protein-like (Domain)
[1489-1555] 2.7e-11 PF00550
PF00550   PP-binding
[1485-1557] 6.40000000000001e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1478-1573] 2.19999900980708e-18 SSF47336
SSF47336   ACP_like
[1486-1556] PS50075
PS50075   ACP_DOMAIN
IPR013968 Polyketide synthase, KR (Domain)
[1197-1374] 6.19999999999994e-37 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-285] 4.40000000000003e-94 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-411] 3.09999999999998e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[570-861] 4.5e-56 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-25] 2.1e-13 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[565-877] 7.40000780398343e-55 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[697-761] 2.89999717862184e-13 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[33-295] 2.99999999999998e-87 G3DSA:3.40.47.10 [297-463] 4.49999999999996e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-463] 8.00002659346736e-100 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1195-1395] 5.69999999999998e-22 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[569-883] 3.99998544139406e-84 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1487-1559] 3.69999438558106e-22 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-463] SM00825
SM00825   PKS_KS
SignalP No significant hit
TMHMM No significant hit
Tetrn_00100 Tetrn_00100   Tetrn_00120 Tetrn_00120
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