Tetrn_00100 : CDS information

Location

Organism	Streptomyces sp.
Strain	NRRL 11266
Entry name	Tetronomycin
Contig	AB193609
Start / Stop / Direction	25,254 / 23,752 / - [in whole cluster] 25,254 / 23,752 / - [in contig]
Location	complement(23752..25254) [in whole cluster] complement(23752..25254) [in contig]
Type	CDS
Length	1,503 bp (500 aa)

Annotation

Category	3.3 modification reduction
Product	putative oxidoreductase
Product (GenBank)	putative FAD-dependent monooxygenase
Gene
Gene (GenBank)	tmn9
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q1MX79 PmId [18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008) comment Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。 tmn9(500 aa): FAD-dependent oxygenase tmn9の不活化変異株ではTMN生産が消失することが示されている。また、相同性よりflavin-linked oxygenaseと予測している。
Related Reference	ACC Q58PK7 PmId [16148009] Ablation of the otcC gene encoding a post-polyketide hydroxylase from the oxytetracyline biosynthetic pathway in Streptomyces rimosus results in novel polyketides with altered chain length. (J Biol Chem. , 2005) comment <Anhydrotetracycline oxygenase (otcC), Streptomyces rimosus, BLAST 12; id=36%> otcC遺伝子がpolyketide backboneの生合成後、anthracycline structureのC-6位をhydroxyl化するanhydrotetracycline oxygenaseをエンコードしていること、また、otcC破壊株では新規のC-17 polyketideが生じることから、otcC遺伝子産物が無いことによりOxytetracycline (OTC)の“minimal”polyketide synthaseに影響を及ぼすことが示されている。

Sequence

>putative oxidoreductase [putative FAD-dependent monooxygenase]
MSEPVVVVGAGPAGLMLACELAMRDVPAVLVDIHPTQRAEAPAMAINAGTLEMLDQRGLA
AGLREGTVTFPEVRFADLRLAFEKVQGPREPTHMVLQSRLEKVLIDRAVELGVDLRWATR
LTGFEEAADGSGVTVTLASDAGEEQLRCRYLVGCDGRESIVRKQAGIDYVGDDWVIVRGI
VGDVAINREDVAPEQYGLSYTDNGDQFLGAPLSPDVMRVFSAEFSTEPPEFEDGPATLEQ
LGDAVKRLTGKELKATEAHWLQHYSIVTRNAEQYRKGRVFIAGDAAHVHYPYNGQGLGTA
IGDAVNLGWKIAAEVHGWAPADLLDSYHVERHLAGRLACMNIQAQLALLYPRPLARYMRE
MMGEFLKFDEVNVFLAEIVTNLGPAVPIAYEGVPEPVEGDRLLGRRLPKVQIKTADGDMG
VAETLQSGRGVLLDLSGDASAQEESGWPTASTWSAPKPGTDLPGTLLLRPDGCVAWHDGG
GWGQDELRTALRTWFGAPTG

>putative oxidoreductase [putative FAD-dependent monooxygenase]
ATGAGTGAGCCGGTCGTCGTCGTGGGCGCCGGGCCCGCCGGTCTGATGCTCGCCTGCGAG
CTGGCGATGCGCGACGTGCCCGCGGTCCTTGTGGACATCCACCCCACGCAGCGCGCCGAG
GCTCCGGCCATGGCGATCAACGCGGGCACGCTGGAGATGCTGGACCAGCGCGGCCTCGCC
GCCGGGCTGCGGGAAGGCACGGTCACGTTCCCCGAGGTGCGGTTCGCGGACCTGCGGCTG
GCCTTCGAGAAGGTGCAGGGGCCGCGTGAACCCACCCACATGGTGCTCCAGTCGCGGCTG
GAGAAGGTGCTGATCGACCGGGCCGTCGAGCTGGGCGTCGACCTGCGCTGGGCCACCCGC
CTCACCGGGTTCGAGGAGGCAGCGGACGGGTCCGGCGTCACCGTGACGCTCGCCTCCGAC
GCCGGCGAGGAGCAACTGCGCTGCCGGTACCTGGTCGGCTGCGACGGGCGGGAGAGCATC
GTCCGCAAGCAGGCCGGCATCGACTACGTCGGCGACGACTGGGTGATCGTACGCGGCATC
GTCGGCGACGTCGCGATCAACCGCGAGGACGTGGCGCCCGAGCAGTACGGCCTGTCCTAC
ACCGACAACGGCGACCAGTTCCTCGGCGCTCCGCTGAGCCCCGACGTCATGCGCGTGTTC
AGCGCCGAGTTCTCCACCGAGCCGCCGGAGTTCGAGGACGGCCCGGCGACGCTGGAGCAG
CTCGGCGACGCCGTCAAGCGACTGACCGGCAAGGAGCTGAAGGCCACCGAGGCGCACTGG
CTGCAGCACTACTCGATCGTCACCCGCAACGCGGAGCAGTACCGCAAGGGCCGCGTCTTC
ATCGCAGGCGACGCCGCGCACGTGCACTACCCGTACAACGGGCAGGGCCTCGGCACCGCC
ATCGGCGACGCGGTGAACCTCGGCTGGAAGATCGCCGCCGAGGTCCACGGCTGGGCGCCG
GCGGACCTGCTCGACTCGTACCACGTGGAGCGGCACCTCGCCGGCCGGCTCGCCTGCATG
AACATCCAGGCGCAGCTCGCCCTCCTCTACCCGCGCCCGCTGGCCCGCTACATGCGGGAG
ATGATGGGCGAGTTCCTGAAGTTCGACGAGGTGAACGTCTTCCTCGCCGAGATCGTCACC
AACCTCGGCCCCGCCGTCCCCATCGCCTACGAAGGCGTGCCGGAGCCCGTCGAAGGCGAC
CGGCTGCTCGGCCGGCGGCTGCCGAAGGTGCAGATCAAGACCGCGGACGGCGACATGGGC
GTCGCCGAGACCCTGCAGTCCGGCCGCGGTGTGCTGCTCGACCTGTCCGGTGACGCCTCC
GCGCAGGAGGAGAGCGGCTGGCCGACCGCGTCGACGTGGTCCGCGCCCAAGCCCGGTACC
GACCTGCCGGGCACCCTGCTGCTGCGCCCGGACGGCTGCGTCGCCTGGCACGACGGCGGC
GGCTGGGGCCAGGACGAGCTGCGCACCGCCCTGCGCACCTGGTTCGGCGCCCCGACCGGC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3 IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE
SignalP	No significant hit
TMHMM	No significant hit

Tetrn_00090 Tetrn_00110