Virg_00050 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB621357
Start / Stop / Direction	11,087 / 10,101 / - [in whole cluster] 17,290 / 18,276 / + [in contig]
Location	complement(10101..11087) [in whole cluster] 17290..18276 [in contig]
Type	CDS
Length	987 bp (328 aa)

Annotation

Category	5.1 general function
Product	putative branched-chain alpha-keto acid dehydrogenase E1 beta subunit
Product (GenBank)	putative branched-chain alpha keto acid dehydrogenase E1 subunit beta
Gene	bkdD bkdB
Gene (GenBank)	bkdD
EC number	1.2.4.4
Keyword
Note	It is supposed that this ORF is not involved in both virginiamycin M and virginiamycin S biosynthesis.
Note (GenBank)
Reference	ACC G1UHX5 PmId [21816878] VisG is essential for biosynthesis of virginiamycin S, a streptogramin type B antibiotic, as a provider of the nonproteinogenic amino acid phenylglycine. (Microbiology. , 2011) comment VisG機能解析の報告。 orf7-visFの8ORFsをvirginiae butanolide regulonとして提唱している。 branched-chain a-keto acid dehydrogenase (BCDH) complexは、oxidative decarboxylationに関与する。本ORFの並び(Virg_00060)にE1 alpha subunit(bkdC)があり、complexは揃っている。 visG欠損とbkdD欠損のdouble mutant はvirginiamycin S生産能が見られなかった(results not shown)。 visG欠損株でもvirginiamycin S生産はみられなかったが、virginiamycin Mは生産が確認された。また、この株においてvisG導入でvirginiamycin Mの生産への変化なく、virginiamycin S生産がWTと同等量の発現が見られたことから、bkdDはvirginiamycinsの生合成には関与していないと結論づけている。
Related Reference	ACC Q53593 PmId [8002616] ( , ) comment Streptomyces avermitilis E1-beta branched-chain alpha keto acid dehydrogenase_bkdB branched-chain alpha-keto acid dehydrogenase(BCDH) complex BCDH and decarboxylase(E1[ab]), dihydrolipoamide acyltransferase (E2)で構成。論文中ではE1[alpha+beta]+E2だけでも、BCDH活性があることがわかっている。 avermectinのポリケタイド骨格のスターター分子であるS(+)-alpha-methylbutyric acidやisobutyric acidのacyl-coenzyme A formを作るのに必要だとしている。

Sequence

>putative branched-chain alpha-keto acid dehydrogenase E1 beta subunit [putative branched-chain alpha keto acid dehydrogenase E1 subunit beta]
MSEITMAKALNTALRDALRDDPRTILFGEDIGALGGVFRITDGLAAEFGDERCFDTPLAE
SAILGTAVGMAMYGYRPVVEMQFDAFAYPAFEQLVSHVAKLRNRTRGAIGLPLTIRIPYG
GGIGGVEHHSDSSEIYYMATPGLTVVTPATAADAYSLLRRSIASPDPVVFLEPKRLYWRK
EALGLPVDTGPLGSAVIRRHGTHATLIAYGPAVTTALEAAEAAAEHGWDLEVIDLRTLMP
LDDATVCASVRRTGRAVVVHEAHGFAGPGAEIAARITERCFYHLEAPVRRVTGFDVPYPP
PLLERHYLPGVDRILDAVASLEWEAVPA

>putative branched-chain alpha-keto acid dehydrogenase E1 beta subunit [putative branched-chain alpha keto acid dehydrogenase E1 subunit beta]
ATGTCCGAGATCACCATGGCCAAGGCGCTCAACACCGCCCTGCGCGACGCCCTGCGCGAC
GACCCCAGGACCATCCTGTTCGGCGAGGACATCGGTGCCCTCGGAGGCGTCTTCCGGATC
ACCGACGGGCTGGCCGCCGAATTCGGCGACGAACGCTGCTTCGACACCCCGCTCGCCGAA
TCGGCCATCCTGGGCACCGCCGTCGGCATGGCGATGTACGGCTACCGCCCGGTCGTCGAA
ATGCAGTTCGACGCCTTCGCCTACCCCGCCTTCGAACAGCTCGTCAGCCACGTCGCCAAA
CTGCGCAACCGCACGCGCGGCGCCATCGGCCTCCCCTTGACCATCCGCATCCCGTACGGC
GGCGGCATCGGCGGCGTCGAGCACCACAGCGACTCCTCCGAGATCTACTACATGGCCACC
CCCGGACTGACGGTCGTCACCCCGGCGACCGCGGCCGACGCGTACTCGCTGCTGCGTCGT
TCCATAGCCTCCCCCGACCCCGTGGTCTTCCTCGAACCCAAGCGGCTGTACTGGCGCAAG
GAGGCCCTCGGACTGCCGGTGGACACCGGCCCGCTCGGCTCGGCGGTCATAAGACGGCAC
GGCACCCATGCCACCCTCATCGCCTACGGCCCCGCGGTCACCACCGCGCTGGAAGCCGCC
GAGGCCGCAGCCGAGCACGGCTGGGACCTCGAAGTCATCGACCTGCGGACCCTGATGCCG
CTCGACGACGCGACGGTCTGCGCCAGCGTGCGCCGTACCGGACGCGCCGTCGTCGTCCAC
GAGGCGCACGGCTTCGCCGGCCCGGGCGCCGAGATCGCCGCCCGGATCACCGAACGCTGC
TTCTACCACCTGGAAGCACCCGTGCGCCGCGTCACCGGATTCGACGTGCCGTACCCGCCG
CCGCTGCTGGAGCGCCACTACCTGCCCGGCGTGGACCGGATCCTGGACGCGGTGGCGTCC
CTGGAATGGGAGGCGGTCCCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005475 Transketolase-like, pyrimidine-binding domain (Domain) PF02779 Transket_pyr SM00861 Transket_pyr IPR005476 Transketolase, C-terminal (Domain) PF02780 Transketolase_C IPR009014 Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II (Domain) SSF52922 Transketo_C_like IPR015941 Transketolase-like, C-terminal (Domain) G3DSA:3.40.50.920 Transketo_C_like
SignalP	No significant hit
TMHMM	No significant hit

Virg_00040 Virg_00060