Virg_00130 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB283030
Start / Stop / Direction	41,580 / 42,569 / + [in whole cluster] 13,204 / 14,193 / + [in contig]
Location	41580..42569 [in whole cluster] 13204..14193 [in contig]
Type	CDS
Length	990 bp (329 aa)

Annotation

Category	4.1 transcriptional regulator
Product	transcriptional activator
Product (GenBank)	pathway specific regulatory protein
Gene
Gene (GenBank)	vmsS
EC number
Keyword	SARP family
Note	This ORF is involved in both virginiamycin M and virginiamycin S biosynthesis.
Note (GenBank)
Reference	ACC A4PHL8 PmId [17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007) [19332826] Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR. (Microbiology. , 2009) comment [PMID:17350183] virginiamycin M生合成gene clusterの報告。 putative regulators (vmsS, vmsT)のクローニングをしている。 vmsS はSARP family transcriptional activatorsに相同性を示す。 VmsTはbacterial two-component signal transduction systemのresponse-regulator proteinsに相同性を示す。 VmsSとVmsTは、highly complex regulatory cascadeだと推測している。 [PMID:19332826] vms genesの転写解析報告。 virN-virM-vmsS operon VmsSとVmsTは一次代謝や形態分化には関与していないことが示され、また、VmsS欠損株の解析によりvirginiamycin Mとvirginiamycin Sを生産しないことから、VmsSは両生合成に関与するとしている。 virginiamycin clusterの制御領域にあるVmsR欠損株の転写解析により、vmsSとvmsTの発現はvmsR制御下にあり、これら3つのpathway-specific regulatorsによってvirginiamycin biosynthesisが制御されていると示唆している。 vmsSは、virK,virJ,virI,virH,virG,virF,virE,virD,visB,visC,visDの11gensのmajor transcriptional activatorとして機能すると考察している。
Related Reference	ACC P16922 PmId [2294088] Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2). (J Bacteriol. , 1990) comment Streptomyces coelicolor transcriptional regulator RedD redDの配列解析と機能解析をし、転写活性も見ている。

Sequence

>transcriptional activator [pathway specific regulatory protein]
MQFRILGPVEIHDERRGLRIVPTGSKQRVLLGALVVKAGHLVSYHRLIDELWGEHPPANP
ANALQAHVARLRKLLEDAGSTGQERIDTHALGYQLHTGPDATDAGRFHRLCAQGRAVIAT
DPERAVHLLRQALALWRGPALQGSLGGDICLAEAAQLEENRLVALETLYEAGLRAGLHHE
ITGELEELIGDNPVRERFYELLMVALHRCGRQAEALGTYERARTRLADELGVEPGAALHR
RVRSILSPGREQPAPAAARSAAPMVRSLPPRPDTAGRRAASTAPRGGAVVLDLGDELARL
RRRLENLTREQEDLARRMDQLTARTAAAR

>transcriptional activator [pathway specific regulatory protein]
ATGCAGTTCCGCATTCTCGGCCCTGTAGAGATCCACGACGAGCGGCGCGGCCTGCGGATC
GTACCCACGGGCTCCAAGCAGCGCGTCCTGCTCGGCGCCCTGGTCGTGAAGGCCGGCCAT
CTGGTGTCGTACCACCGCCTGATCGACGAGTTGTGGGGCGAACACCCGCCGGCGAACCCG
GCCAACGCCCTCCAGGCCCACGTCGCCCGGCTCAGGAAGCTGTTGGAGGACGCCGGATCG
ACCGGTCAGGAGCGGATCGACACCCACGCGCTGGGCTACCAGCTGCACACCGGGCCGGAC
GCGACCGACGCCGGCAGGTTCCACCGGCTGTGCGCCCAGGGCCGGGCCGTGATCGCCACC
GACCCCGAGCGGGCCGTCCACCTGCTGCGCCAGGCACTCGCCCTGTGGCGGGGGCCCGCG
TTGCAGGGCAGCCTCGGCGGGGACATCTGCCTGGCGGAGGCCGCCCAGCTGGAGGAGAAC
CGGCTGGTCGCCCTGGAAACGCTCTACGAGGCCGGCCTGCGGGCGGGCCTGCACCACGAG
ATCACCGGCGAGCTGGAGGAGCTCATCGGTGACAATCCGGTGCGGGAGCGGTTCTACGAG
CTGCTGATGGTCGCCCTCCACCGCTGCGGCCGGCAGGCGGAGGCGCTCGGCACGTACGAG
CGGGCCCGGACGCGTCTGGCCGATGAACTCGGGGTGGAACCCGGTGCGGCGCTGCACCGA
CGGGTCCGGTCGATCCTCAGCCCCGGCCGCGAGCAGCCGGCCCCCGCTGCGGCGCGCTCG
GCGGCGCCGATGGTCCGCTCCCTCCCGCCACGGCCCGACACCGCTGGTCGGCGGGCTGCA
TCGACGGCTCCCCGGGGCGGGGCCGTCGTCCTCGACCTCGGCGACGAACTGGCGCGACTG
CGAAGGCGGTTGGAGAACCTGACCCGCGAGCAGGAGGACCTGGCCCGCCGGATGGACCAG
CTCACCGCCCGCACCGCGGCCGCCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) PF00486 Trans_reg_C SM00862 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) SM01043 BTAD PF03704 BTAD IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Virg_00120 Virg_00140