Virg_00120 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB283030
Start / Stop / Direction	40,372 / 41,529 / + [in whole cluster] 11,996 / 13,153 / + [in contig]
Location	40372..41529 [in whole cluster] 11996..13153 [in contig]
Type	CDS
Length	1,158 bp (385 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	Sarcosine oxidase
Gene
Gene (GenBank)	virM
EC number
Keyword
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC A4PHL7 PmId [17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007) [19332826] Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR. (Microbiology. , 2009) comment [PMID:17350183] virginiamycin M生合成gene clusterの報告。 virginiamycin M1からvirginiamycin M2への変換のためにC26 positionでのhydroxylation and dehydrogenationをVirM and/or VirNが行うかもしれないと推測しているが明らかにはなっていない。 [PMID:19332826] vms genesの転写解析報告。 virN-virM-vmsS operon vmsRにより発現が制御される。
Related Reference	ACC P40859 PmId [10913293] Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue. (Biochemistry. , 2000) [10220347] Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase. (Biochemistry. , 1999) comment Bacillus sp. B-0618 Monomeric sarcosine oxidase(sox) [PMID: 10913292] MSOXのカイネティクスを見ている。 MSOXは共有結合のflavin-sulfite複合体を形成するが、複合体形成に対するsignificant kinetic barrierが存在する。 [PMID: 10220347] Cys315がMSOXのcovalent FAD attachment siteとして同定した。このORF(virM)では保存されていない。

Sequence

>putative oxidoreductase [Sarcosine oxidase]
MARTATGLDAEAAVVGLGAWGACSLWQLARQGVDVIGIDRYGIGNVHGSSYGESRMFRTA
CLEHPGLVPLAQRSRELWAELEEQTGRVLMERTGAMLIGPPDGRIVGGALRAAREHRLDI
ELLDPASMRERVPAHAGLPDDHVGVLEPAGGLTYPEHTIAAAVDAARAAGARVVTDTRVT
AVEPGNDGIVVRTALRTLRVARLVVAAGPWLSQLVPGLPLDVLRMPTTWFTPAEPDPRFT
LERLPVFMRELADGNVIWGHGTLPGGTEVKLGLEDGGRHFRVVDPENMDRSVSPADWSVL
SRILPAAVPGLGDTPSRATANLYARTPDGQFLLGPLRHDPRIIVAGGCNSHGFKHATGIG
EAVADMVRGRTPAVPLGFADPNSLR

>putative oxidoreductase [Sarcosine oxidase]
ATGGCCCGTACAGCCACCGGCCTGGACGCCGAGGCCGCCGTCGTGGGGCTGGGCGCCTGG
GGCGCCTGCTCCCTGTGGCAGCTGGCCCGGCAGGGGGTCGACGTCATCGGCATCGACCGC
TACGGCATCGGCAACGTGCACGGTTCCTCGTACGGCGAGAGCCGCATGTTCCGCACCGCC
TGCCTGGAGCACCCGGGCCTGGTCCCGCTCGCGCAGCGCTCGCGCGAGCTGTGGGCCGAG
CTGGAGGAGCAGACCGGCCGGGTGCTGATGGAGCGGACCGGGGCCATGCTGATCGGCCCG
CCGGACGGGCGCATCGTCGGCGGCGCGCTGCGGGCGGCCCGCGAGCACCGGCTGGACATC
GAGCTGCTCGACCCGGCGAGCATGCGCGAGCGGGTCCCCGCGCATGCCGGGCTGCCGGAC
GACCATGTGGGCGTGCTGGAGCCGGCCGGCGGGCTGACCTACCCCGAGCACACCATCGCC
GCCGCGGTCGACGCGGCGCGGGCGGCCGGGGCCCGGGTGGTCACCGACACCCGGGTCACC
GCGGTCGAACCCGGCAACGACGGCATCGTGGTGCGCACCGCGTTGCGGACGCTCCGTGTC
GCCCGGCTCGTGGTGGCCGCGGGCCCGTGGCTGTCCCAGCTGGTGCCGGGGCTCCCCCTC
GACGTGCTGCGCATGCCCACGACCTGGTTCACCCCGGCCGAGCCCGATCCGCGGTTCACC
CTGGAGCGGCTGCCGGTGTTCATGCGCGAACTCGCCGACGGGAACGTGATCTGGGGCCAC
GGCACCCTGCCCGGCGGCACCGAGGTGAAGCTCGGGCTGGAGGACGGCGGACGCCACTTC
CGCGTCGTCGACCCCGAGAACATGGACCGCTCGGTCTCCCCCGCCGACTGGTCGGTGCTC
TCCCGCATCCTCCCGGCGGCCGTGCCCGGCCTCGGCGACACCCCCTCGCGGGCGACGGCC
AACCTGTACGCGCGCACCCCCGACGGCCAGTTCCTCCTCGGCCCGCTGCGGCACGACCCG
CGGATCATCGTCGCGGGCGGGTGCAACAGCCACGGTTTCAAGCACGCGACCGGGATCGGC
GAGGCCGTCGCCGACATGGTCCGCGGCAGGACCCCGGCCGTCCCGCTCGGTTTCGCTGAC
CCGAACTCCCTGCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006076 FAD dependent oxidoreductase (Domain) PF01266 DAO
SignalP	No significant hit
TMHMM	No significant hit

Virg_00110 Virg_00130