Virg_00140 Virg_00140   Virg_00160 Virg_00160

Virg_00150 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction45,203 / 44,295 / - [in whole cluster]
16,827 / 15,919 / - [in contig]
Locationcomplement(44295..45203) [in whole cluster]
complement(15919..16827) [in contig]
TypeCDS
Length909 bp (302 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative phosphopantetheinyl transferase
Product (GenBank)4-phosphopantetheinyl transferase
Gene
Gene (GenBank)virK
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007)
comment
[PMID:17350183]
virginiamycin M生合成gene clusterの報告。

auxiliary genes,tailoring genesを構成するvir cluster(virB, virC, virD, virE, virI, virJ, virK)の一つ。

本ORFの機能実験は行われていないが、相同性より4-phosphopantetheinyl transferaseと予測している。
Related Reference
ACC
Q9RFL1
NITE
Myxo_00010
PmId
[10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999)
[11134965] The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases. (J Biochem. , 2001)
comment
[PMID: 10601310]
Myxothiazol生合成gene clusterの同定論文。
MtaA: Ppant transferase (Ppant=4'-phosphopantetheinyl)
4'-phosphopantetheinyl transferase MtaAによってmyxothiazol合成は活性化される。
mtaAへのkanamycin resistance geneの挿入はmyxothiazol合成を阻害する。
MtaB-MtaGのACP, PCP domainは4'-phosphopantetheine cofactorのためのputative binding siteのProsite consensus signatureを含んでいる。
--
[PMID:11134965]
MtaAをACP, PCPと異種性発現して、4'-phosphopantetheinyl transferases機能があることを確認。
MtaAは他種のACP, PCPも同様に加工でき、基質特異性がゆるい。

close this sectionSequence

selected fasta
>putative phosphopantetheinyl transferase [4-phosphopantetheinyl transferase]
MSARAAAGARRPADGEVRLWVCANDDLGPGTATALAGHWLDEHEQATADRFIFERDRRQY
LVAHVLVRRLLSLETGVPEALIELRRSERGRPSVRPPEGGWPAAQGPLDFNLSHARGYNA
IAVGRGRRVGVDVERLDRVSSRALDGIAESFTPGERVQLARTPAEVYDRTVLRLWTLKEA
YAKARGLGLGLPFDSFGFVLSDNGGVAAFEPPADDPSGTWWLLELEPEPGVLVSLAVEDG
ATPMTALRLHTGFPWRPSALRASIHPGNPVPLLAPGTLSAALPLAGAAPHRARPTELQRS
VA
selected fasta
>putative phosphopantetheinyl transferase [4-phosphopantetheinyl transferase]
ATGAGCGCGCGGGCGGCCGCAGGCGCACGGCGGCCCGCCGACGGCGAGGTCAGGCTCTGG
GTGTGCGCCAACGACGACCTCGGGCCGGGCACCGCCACCGCCCTGGCCGGCCACTGGCTG
GACGAGCACGAACAGGCGACCGCGGACCGGTTCATCTTCGAGCGGGACCGCCGCCAGTAC
CTCGTGGCACACGTCCTCGTGCGGCGCCTGCTCTCGCTGGAGACCGGTGTCCCCGAGGCG
CTGATCGAGCTGCGCAGGTCCGAGCGTGGCCGGCCCTCCGTACGGCCGCCCGAGGGCGGC
TGGCCCGCCGCACAGGGCCCGCTGGACTTCAACCTCTCGCACGCCCGCGGATACAACGCG
ATCGCGGTCGGCCGGGGACGCCGGGTCGGGGTCGACGTCGAACGGCTCGACCGGGTGAGC
AGCCGCGCACTGGACGGGATCGCCGAGAGCTTCACCCCCGGCGAGCGCGTCCAGCTGGCC
CGGACACCCGCTGAGGTGTACGACCGAACGGTGCTGCGCCTGTGGACGCTCAAGGAGGCC
TACGCCAAGGCGCGCGGACTCGGCCTCGGACTGCCCTTCGACTCGTTCGGCTTCGTGCTG
TCCGACAACGGCGGGGTGGCCGCGTTCGAACCGCCGGCCGACGACCCGTCGGGAACCTGG
TGGTTGCTCGAACTCGAACCCGAGCCCGGGGTGCTGGTCTCCCTCGCGGTCGAGGACGGG
GCCACCCCGATGACCGCGCTCAGGCTGCACACGGGCTTCCCCTGGCGGCCCTCCGCCCTG
CGCGCGAGCATCCACCCCGGGAACCCCGTCCCGCTGCTCGCCCCCGGGACCCTCTCCGCC
GCGCTCCCCCTCGCCGGGGCCGCGCCCCACCGAGCGCGCCCCACCGAACTCCAGAGGAGC
GTCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008278 4'-phosphopantetheinyl transferase (Domain)
[17-133] 9.59999914716171e-24 SSF56214 [127-259] 1.70000295590054e-24 SSF56214
SSF56214   4-PPT_transf
[128-203] 4.40000000000001e-13 PF01648
PF01648   ACPS
[128-237] 1.3e-12 G3DSA:3.90.470.20
G3DSA:3.90.470.20   G3DSA:3.90.470.20
SignalP
[1-5] 0.385 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Virg_00140 Virg_00140   Virg_00160 Virg_00160
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