Virg_00170 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB283030
Start / Stop / Direction	46,923 / 46,036 / - [in whole cluster] 18,547 / 17,660 / - [in contig]
Location	complement(46036..46923) [in whole cluster] complement(17660..18547) [in contig]
Type	CDS
Length	888 bp (295 aa)

Annotation

Category	1.4 Other
Product	putative trans-acting acyltransferase
Product (GenBank)	acyltransferase
Gene
Gene (GenBank)	virI
EC number
Keyword	virginiamycin M trans AT
Note
Note (GenBank)
Reference	ACC A4PHM2 PmId [17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007) comment virginiamycin M生合成gene clusterの報告。 auxiliary genes,tailoring genesを構成するvir cluster(virB, virC, virD, virE, virI, virJ, virK)の一つ。このクラスター内に唯一存在するacyltransferase。 virIの過剰発現株は、Virginamycin M生産が増加した。各PKSにおいて、KS domainのすぐ下流にonly AT-docking siteが存在していた。また、系統解析によると、VirIはmalonyl-CoA-specific discrete ATsに分類された。 isobutyryl-CoAがchain initiationに対してどのようにPKS systemへtransferされるか、また、VirA proteinのACP-loading domainとの付着様式については不明であると述べている。ポリケチド生合成のためのAT less type I PKSやdiscrete ATはいくつかの微生物で検出されているが、streptomyces spiciesにおけるポリケチド生合成においてdiscrete and iterative ATはまれであると記述あり。
Related Reference	ACC O34825 PmId [17234808] The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. (Proc Natl Acad Sci U S A. , 2007) comment Bacillus subtilis Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase pksC PksX cluster内にある3つのacyltransferaseのうちの一つ。 PksCはMal-CoAに選択的であり、Malだけが唯一PksC上でloadされたのを確認している(data not shown)。さらに、MalがPksCからAcpKへtransferされる。

PKS/NRPS Module

M	10		malonyl-CoA not conserved HAFHS(H->G,A->P)

AT	4..283

Sequence

>putative trans-acting acyltransferase [acyltransferase]
MTRIFMFPGQGAQRVGMGRTLLDRFPDLEREASDTLGYSLRRLCLEDPEGRLGNTRYTQP
AMFAVNALAHRAAVEDGARPDIAIGHSLGEYNALEAAGVFGFTDGLRLVAARAAAMAEVG
GGGMSAVVGLTETKLRFLLLRAGFATLDLANLNTASQTVLAGPLEDLEEAGQVLEDAGAR
MVRRLDVSGPFHSRYMAPAAAALVPLVRSARLRPPAFPVIANRTAQPYRAELAADLLLQQ
IDHPVRWHETVRTLLDEPDAVFTEIGESTVLTSMVRQIKRDAEPARAARPRQAVA

>putative trans-acting acyltransferase [acyltransferase]
ATGACCCGGATCTTTATGTTTCCCGGACAGGGAGCGCAGCGCGTCGGCATGGGCCGGACG
CTTCTCGACCGCTTCCCCGACCTCGAACGCGAGGCCAGCGACACGCTCGGCTACAGCCTG
CGCCGGCTGTGCCTGGAGGACCCCGAGGGCAGGCTCGGCAACACCCGCTACACCCAGCCC
GCGATGTTCGCCGTGAACGCCCTCGCCCACCGCGCGGCCGTCGAGGACGGGGCCCGCCCC
GACATCGCGATCGGTCACAGCCTGGGTGAGTACAACGCCCTGGAAGCCGCCGGGGTCTTC
GGCTTCACCGACGGCCTGCGGCTGGTCGCGGCCCGCGCCGCGGCCATGGCCGAGGTGGGC
GGCGGAGGGATGAGCGCGGTCGTCGGGCTCACCGAGACCAAGCTGCGCTTCCTGCTCCTG
CGCGCCGGATTCGCAACGCTGGACCTCGCCAACCTCAACACCGCGAGCCAGACCGTCCTG
GCCGGGCCGCTGGAGGACCTGGAGGAAGCAGGCCAGGTACTGGAGGACGCCGGCGCGCGC
ATGGTCCGCCGACTGGACGTCAGCGGCCCCTTCCACTCCCGGTACATGGCGCCCGCCGCC
GCCGCGCTCGTCCCGCTGGTCCGGTCCGCCCGGCTCCGGCCACCGGCCTTCCCCGTGATC
GCCAACCGCACCGCGCAGCCGTACCGCGCAGAGCTGGCCGCCGACCTGCTGCTCCAGCAG
ATCGACCACCCCGTGCGCTGGCACGAGACCGTCCGCACCCTGCTCGACGAGCCTGACGCG
GTGTTCACCGAGATCGGCGAGAGCACCGTGCTGACCAGCATGGTCCGGCAGATCAAGCGG
GACGCCGAGCCCGCCCGCGCGGCCCGCCCGCGCCAGGCCGTCGCCTGA

[10] AT	4..283

[10] malonyl-CoA not conserved HAFHS(H->G,A->P)	189..193

[10] AT	10..849

[10] malonyl-CoA not conserved HAFHS(H->G,A->P)	565..579

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR004410 Malonyl CoA-acyl carrier protein transacylase, FabD-type (Family) TIGR00128 FabD IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR024925 Malonyl CoA-acyl carrier protein transacylase (Family) PIRSF000446 Mct
SignalP	No significant hit
TMHMM	No significant hit

Virg_00160 Virg_00180