Virg_00300 Virg_00300   Virg_00320 Virg_00320

Virg_00310 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction98,512 / 99,285 / + [in whole cluster]
36 / 809 / + [in contig]
Location98512..99285 [in whole cluster]
36..809 [in contig]
TypeCDS
Length774 bp (257 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.6 biosynthesis of butyrolactone
Productgamma-butyrolactone biosynthesis enzyme
6-dehydro-virginiae butanolide A reductase
Product (GenBank)Orf4
GenebarS1
Gene (GenBank)orf4
EC number
Keyword
Note
Note (GenBank)
  • ketoacyl ACP/CoA reductase homolog
Reference
ACC
PmId
[10792718] Identification of an AfsA homologue (BarX) from Streptomyces virginiae as a pleiotropic regulator controlling autoregulator biosynthesis, virginiamycin biosynthesis and virginiamycin M1 resistance. (Mol Microbiol. , 2000)
[12193632] barS1, a gene for biosynthesis of a gamma-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species. (J Bacteriol. , 2002)
comment
[PMID:10792718]
barXの機能解析論文。

orf4はactinorhodin polyketide chainのassembleにおいてbeta-keto groupを還元するbeta-ketoacyl--ACP reductase と弱い相同性を示す。活性残基やNAD+ binding siteはこのORFでも保存している。


[PMID:12193632(2002)]
barS1の機能解析の報告

本論文にてORF名を orf4->barS1　変更している。
recombinant酵素精製し、(±)-6-dehydro-VB-Aに対しての酵素活性を見ている。また、barS1欠損株は、virginiae butanolide(VB),virginiamycin生産能が欠けていた。VBの外因的添加によりvirginiamycin生産能が回復したことから、virginiamycinの生産にVBの生産が影響すると推測した。

酵素学的解析からBarS1は、VB biosynthesisの最終ステップ6-dehydro-VB-A->VB-Aへの変換に関与する唯一の6-dehydro-VB-A reductaseであると示した。

close this sectionSequence

selected fasta
>gamma-butyrolactone biosynthesis enzyme [Orf4]
MTDRQGLLTDRIALITGASSGIGAAQRGLFAREGAAVVVTARREERLAGLVDELRAQGAR
AAYVVADVTRSEDAVRAVEFTVERFGRLDAAFNKRRHGAGRTPLHLMDDPVYDDIMDTNV
RGVFNCLRPEIAAMLASGAGGSIVNTSSTGGLVATPVAAPYVVSKHAVLGLTKGPAAEYG
AHGIRVNAIAPGTTRSEMVADWFAQNPDAEELLHRATPQPRTAEPQEIAEAAAWLCSERA
SFVTGSTLVVDGGFTIL
selected fasta
>gamma-butyrolactone biosynthesis enzyme [Orf4]
ATGACTGATCGACAAGGGCTTCTGACAGACAGGATCGCACTGATAACGGGAGCCAGCAGC
GGCATCGGCGCGGCGCAGCGCGGGTTGTTCGCCAGGGAGGGCGCGGCGGTGGTGGTCACC
GCCCGCCGCGAGGAGCGGCTGGCCGGGCTGGTGGACGAGTTGCGGGCGCAGGGCGCCCGA
GCCGCATACGTGGTCGCCGACGTGACCCGGTCCGAGGACGCGGTACGTGCCGTCGAGTTC
ACGGTGGAACGTTTCGGCAGGCTCGATGCCGCGTTCAACAAACGCCGGCACGGGGCCGGC
CGCACACCGCTGCACCTGATGGACGACCCGGTCTACGACGACATCATGGACACCAACGTG
CGCGGGGTGTTCAACTGCCTGCGCCCGGAGATCGCGGCGATGCTCGCATCCGGAGCAGGC
GGTTCGATCGTCAACACCAGCAGCACCGGCGGCTTGGTCGCCACTCCGGTCGCCGCGCCG
TACGTGGTGTCCAAGCACGCGGTGCTCGGTCTGACCAAAGGGCCTGCCGCCGAGTACGGC
GCCCACGGCATTCGGGTCAACGCGATAGCCCCCGGCACGACCCGCAGCGAGATGGTGGCG
GACTGGTTCGCGCAGAACCCGGATGCCGAGGAGCTGCTGCACCGGGCCACGCCGCAGCCC
CGTACCGCCGAACCCCAGGAGATCGCCGAGGCCGCTGCGTGGCTGTGCAGCGAGCGGGCG
TCCTTCGTCACCGGGTCGACCCTGGTGGTCGACGGCGGGTTCACCATCCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002347 Glucose/ribitol dehydrogenase (Family)
[12-29] 7.60000011115812e-32 PR00081 [135-151] 7.60000011115812e-32 PR00081 [161-180] 7.60000011115812e-32 PR00081 [182-199] 7.60000011115812e-32 PR00081 [218-238] 7.60000011115812e-32 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[5-256] 4.59999999999996e-77 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[148-176] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Virg_00300 Virg_00300   Virg_00320 Virg_00320
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