Virg_00320 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB035548
Start / Stop / Direction	100,271 / 99,291 / - [in whole cluster] 1,795 / 815 / - [in contig]
Location	complement(99291..100271) [in whole cluster] complement(815..1795) [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	4.6 biosynthesis of butyrolactone
Product	putative gamma-butyrolactone biosynthesis enzyme
Product (GenBank)	Orf5
Gene	barS2
Gene (GenBank)	orf5
EC number
Keyword
Note
Note (GenBank)	dNDP-glucose dehydratase homolog
Reference	ACC Q9LBV2 PmId [18034227] Identification by gene deletion analysis of barS2, a gene involved in the biosynthesis of gamma-butyrolactone autoregulator in Streptomyces virginiae. (Arch Microbiol. , 2008) comment barS2の機能解析報告。本論文にてORF名をorf5からbarS2へ変更している。 BarS2はNAD-dependent epimerase/dehydratase familyに属すると予測。 barS2欠損株の解析から、virginiae butanolideの生合成に関与することを示した。 BarS2はdehydration stepを触媒するNAD-dependent dehydrataseとして機能するかもしれないと考察している。
Family/Domain	FD IPR001509 comment [IPR001509] NAD-dependent epimerase/dehydratase (Domain)

Sequence

>putative gamma-butyrolactone biosynthesis enzyme [Orf5]
GSGSGHGSTGSSAAGRRRRPYAPGRPPRRGGTL-PAPP-KDPAVRYVHADLTDSASLYGV
CDGIDIVLHCAARVGGPEHSARAVNEEGTRALLAEAERAGVRRFVQVGTASVYGRGVFRN
AGPEHLPVVPQSPTSRTRAAAERLVLAAGGTVLRPHLVIGPATAWVVPGLVRLHQRLAAS
VDGWRARMSVVDVDDLARAVIAVAVAPQDTCGVWHANHPEPVGCARIFDALVRLLGLPAG
TGDIEAGGARRLLAEHPVLLHHLAMLTTDHWFASSRLWRETGCPAGARSRRASPATPRGT
AN-LATDTAGRPGRRACRKSGRSSGGS

>putative gamma-butyrolactone biosynthesis enzyme [Orf5]
GGATCCGGATCTGGTCACGGATCGACCGGTTCATCGGCGGCAGGGCGCCGCCGCCGCCCG
TACGCTCCCGGGCGCCCGCCTCGTCGTGGCGGGACGCTCCNACCGGCCCCGCCGNAAAAG
GACCCCGCGGTCCGGTACGTCCACGCCGACCTCACCGACTCGGCAAGCCTGTACGGCGTG
TGCGACGGCATCGACATCGTGCTCCACTGCGCGGCGCGGGTCGGCGGCCCGGAGCACTCG
GCCCGGGCGGTCAACGAGGAGGGCACCCGGGCACTGCTGGCCGAGGCTGAACGGGCCGGA
GTCCGCCGCTTCGTCCAGGTCGGCACCGCCTCCGTGTACGGCCGGGGGGTGTTCCGGAAC
GCCGGACCCGAGCACCTCCCCGTCGTGCCGCAATCGCCCACCAGCCGGACCCGTGCCGCC
GCCGAGAGGCTCGTGCTCGCGGCCGGCGGGACGGTGCTGCGCCCGCACCTGGTCATCGGG
CCGGCGACCGCCTGGGTGGTCCCGGGTCTGGTCCGCCTCCACCAGCGGCTCGCGGCCTCC
GTCGACGGCTGGCGGGCCCGGATGTCGGTGGTCGACGTCGACGACCTCGCCCGTGCGGTG
ATCGCCGTCGCCGTCGCCCCCCAGGACACCTGCGGCGTCTGGCACGCCAACCACCCCGAA
CCGGTCGGCTGCGCCCGCATCTTCGACGCACTGGTACGCCTGCTCGGCCTGCCCGCGGGC
ACCGGCGACATCGAAGCCGGTGGGGCCCGTCGCCTCCTCGCCGAGCACCCGGTCCTGCTG
CACCACCTCGCCATGCTGACCACCGACCACTGGTTCGCCAGCTCCCGGCTCTGGCGCGAG
ACCGGCTGCCCGGCCGGGGCACGTTCGAGGAGAGCCTCGCCCGCCACGCCTCGTGGTACC
GCGAACNACCTCGCGACGGACACCGCAGGCCGGCCGGGCCGACGGGCATGCCGGAAGTCC
GGGCGGTCCTCCGGCGGATCG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Virg_00310 Virg_00330