Virg_00410 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB072568
Start / Stop / Direction	112,758 / 113,966 / + [in whole cluster] 4,994 / 6,202 / + [in contig]
Location	112758..113966 [in whole cluster] 4994..6202 [in contig]
Type	CDS
Length	1,209 bp (402 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	cytochrome P450 monooxygenase
Gene
Gene (GenBank)	visD
EC number
Keyword	virginiamycin S
Note
Note (GenBank)	VisD
Reference	ACC Q8RR21 PmId [11943483] Characterization of virginiamycin S biosynthetic genes from Streptomyces virginiae. (Gene. , 2002) comment virginiamycin S biosynthetic genesの報告。 visD: cytochrome P450 hydroxylase シーケンス解析、RNAの発現解析、転写開始点の決定を行っている。VisDは相同性から機能を同定しているのみ。 VisD産物はpipecolic acid → 4-oxopipecolic acidへの酸化に関与すると予測された。
Related Reference	ACC P48635 PmId [8416893] Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. (J Bacteriol. , 1993) [7849045] ( , ) comment CPXQ_SACEN(P48635) Saccharopolyspora erythraea_eryK Erythromycin C-12 hydroxylase(EC 1.14.13.154) --- [PMID: 8416893](1993) Saccharopolyspora erythraea (Streptomyces erythraeus)において、P450 monooxygenase familyに属するEryKが、erythromycin生合成の最終反応の１つであるmacrolactone ringのC-12 hydroxylationに関与していることをeryK変異体を作成して調べている。 --- [PMID: 7849045](1995) 不溶性封入体としてE.coliでEryKを過剰産生し、可溶化、再折りたたみ、active holo-EryK再構築をした。動態確認で、erythromycin Bより1200-1900倍もerythromycin Dを基質として好むことが分かった。

Sequence

>putative cytochrome P450 [cytochrome P450 monooxygenase]
MAQQTPPAPPSMADGGKAMLAWLRTMRDEHPVHEDQYGVFHVYRHSDVLAVTSDPAVFSS
DLSRLRPDSSALSEEILSVIDPPLHRKLRSLVSQAFTLRTVADLEPRVTELAGRLLEKVE
GSEFDLVGDFAYPLPVIVIAELLGVPAEDRELFRGWSDRMLSMQVDDPLEIQFGDEAGED
YERLVKEPLKEMHAYLQRHVDARRETPGDDLLSRLVTAEIAGERLTDRQIVEFGALLLMA
GHVSTSMLLGNTVLCLEENPETAAALRADRALISGVIEEVLRMRPPITVAARVTTGEVVV
GGVTIPKDRMVMASLLSANHDERHIQDPEVFDPRRSPNPQLAFGHGIHYCLGGPLARLEG
RVALEMLLDRFEDIRVTPGAPYDFHREGLFVPARSPLTVRRG

>putative cytochrome P450 [cytochrome P450 monooxygenase]
ATGGCCCAGCAGACCCCTCCCGCACCACCGTCCATGGCGGACGGCGGCAAGGCGATGCTC
GCCTGGCTGCGGACGATGCGTGACGAACACCCCGTCCACGAGGACCAGTACGGTGTGTTC
CACGTCTACCGCCACAGCGACGTCCTGGCGGTGACCTCCGACCCCGCCGTCTTCTCCTCC
GACCTGAGCCGGCTGCGTCCGGACTCCAGTGCGCTCAGTGAGGAGATCCTCTCCGTCATC
GACCCGCCGCTCCACCGCAAGCTGCGCAGCCTCGTCAGCCAGGCGTTCACCCTGCGCACC
GTGGCCGACCTGGAGCCTCGTGTCACCGAACTCGCCGGCCGGCTGCTGGAGAAGGTCGAG
GGCAGCGAGTTCGACCTGGTCGGTGACTTCGCCTACCCGCTCCCCGTGATCGTGATCGCC
GAGCTGCTGGGCGTCCCGGCCGAGGACCGCGAGCTGTTCCGCGGTTGGTCCGACCGGATG
CTCTCGATGCAGGTCGACGACCCCCTCGAGATTCAGTTCGGGGACGAGGCGGGCGAGGAC
TACGAGCGGCTGGTCAAGGAGCCGCTCAAGGAGATGCACGCCTACCTCCAGCGGCACGTC
GACGCCCGCCGCGAAACGCCGGGCGACGACCTGCTGTCCCGACTGGTGACGGCGGAGATC
GCCGGCGAGAGGCTGACGGACCGCCAGATCGTCGAGTTCGGCGCGCTGCTGCTGATGGCC
GGGCACGTTTCCACGTCGATGCTGCTCGGCAACACGGTGCTCTGCCTGGAGGAGAACCCC
GAGACCGCCGCCGCGCTGCGGGCCGACCGCGCCCTGATCTCGGGCGTCATCGAGGAGGTC
CTGCGGATGCGGCCCCCGATCACGGTCGCGGCCCGGGTCACCACCGGGGAGGTCGTCGTC
GGCGGGGTGACCATCCCGAAGGATCGGATGGTCATGGCGTCGCTGCTCTCGGCCAATCAC
GACGAGCGGCACATCCAGGACCCGGAGGTCTTCGACCCCAGGCGCAGTCCCAACCCTCAG
CTGGCCTTCGGCCACGGCATCCACTACTGCCTCGGCGGTCCGCTGGCCCGCCTGGAGGGC
AGGGTCGCCCTGGAGATGCTGCTGGACCGGTTCGAGGACATCAGGGTCACTCCGGGCGCG
CCGTACGACTTCCACCGCGAGGGGCTGTTCGTGCCCGCTCGCTCCCCCCTGACCGTCCGT
CGCGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Virg_00400 last last