Virg_00390 Virg_00390   Virg_00410 Virg_00410

Virg_00400 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction111,523 / 112,410 / + [in whole cluster]
3,759 / 4,646 / + [in contig]
Location111523..112410 [in whole cluster]
3759..4646 [in contig]
TypeCDS
Length888 bp (295 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative lysine cyclodeaminase
Product (GenBank)lysine cyclodeaminase
Gene
Gene (GenBank)visC
EC number
Keyword
  • virginiamycin S
  • L-pipecolate
Note
Note (GenBank)
  • VisC
Reference
ACC
PmId
[11943483] Characterization of virginiamycin S biosynthetic genes from Streptomyces virginiae. (Gene. , 2002)
comment
virginiamycin S biosynthetic genesの報告。

visC: lysine cyclodeaminase

シーケンス解析、RNAの発現解析、転写開始点の決定を行っている。VisBは相同性から機能を同定しているのみ。

VisAは3-hydroxypicolinic acidの供給に関与し、VisCはpipecolic acidの供給に関与していると示唆。
Related Reference
ACC
Q54304
NITE
Rapam_00190
PmId
[9473033] Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase. (J Bacteriol. , 1998)
[16536560] Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster. (J Am Chem Soc. , 2006)
comment
Q54304_STRHY
Streptomyces hygroscopicus
Lysine cyclodeaminase_rapL

[PMID: 9473033]
rapLのmutant作成。L-pipecolateを加えた培地で育ったものはrapamycin産生量が回復。

--
[PMID: 16536560]
RapLをE.coliで過剰発現、精製、isotopically labeled substratesを使ってcyclodeaminase反応の機構的詳細(alpha-amineの減少、alpha-carbonにおけるH原子の保持)を確かめている。

close this sectionSequence

selected fasta
>putative lysine cyclodeaminase [lysine cyclodeaminase]
METWVLSERDVTRAVESVGRDALMDRVIDRLTEGLAEVGRGAARSPAARGFNREKPVPGI
WEWMPHRESGDSITLKAVGYSPANPGRFGLPTIIGTVARFDDTTGSLTALIDGVLLTAIR
TGAASAFASRLLAAPDSRVVGLIGSGAQSVTQLHALTRVFPVERVLTWDADPSHAASLAD
RVAFLGLDVQVAEPHRIAAEADIICTATTVPVGGGPVLPADAVFRPHLHINAIGRDLVGK
TELPLDLLRRAHVSPDHPEQARHEGECQQLEPHEIGLSLGELCADPRPPTLCAAD
selected fasta
>putative lysine cyclodeaminase [lysine cyclodeaminase]
ATGGAGACCTGGGTCCTGAGCGAGCGCGACGTGACACGGGCTGTGGAGAGCGTGGGCCGC
GACGCCCTGATGGACCGCGTCATCGACCGCCTCACCGAGGGCCTCGCCGAAGTCGGCCGG
GGCGCCGCGCGCTCTCCCGCAGCGCGCGGATTCAACCGGGAGAAACCGGTCCCGGGTATC
TGGGAGTGGATGCCGCACCGCGAAAGCGGCGACAGCATCACCCTGAAGGCCGTCGGCTAC
AGCCCCGCCAACCCGGGGCGGTTCGGCCTGCCGACCATCATCGGCACGGTCGCCCGCTTC
GACGACACGACCGGCTCGCTGACCGCGCTGATCGACGGCGTGCTGCTCACCGCGATCCGT
ACGGGCGCGGCGAGCGCGTTCGCCAGCCGGCTGCTGGCCGCACCGGACAGCCGCGTGGTC
GGACTCATAGGGTCCGGCGCCCAGTCGGTGACCCAGCTCCACGCCCTCACCCGCGTCTTC
CCTGTCGAGCGGGTGCTCACCTGGGACGCCGACCCGTCGCACGCTGCGAGCCTCGCCGAC
CGGGTGGCGTTCCTCGGCCTCGACGTGCAGGTCGCCGAACCGCACCGGATCGCGGCCGAG
GCCGACATCATCTGCACCGCCACCACCGTCCCGGTCGGCGGCGGGCCCGTCCTGCCGGCC
GATGCCGTGTTCCGCCCCCACCTGCACATCAACGCCATCGGGCGCGACCTGGTCGGCAAG
ACCGAGCTGCCGCTCGACCTGCTGCGGCGGGCGCACGTGAGCCCCGACCACCCCGAGCAG
GCCCGCCACGAGGGCGAGTGCCAGCAGTTGGAGCCGCACGAGATCGGGCTCAGCCTCGGA
GAGCTCTGCGCCGATCCGCGGCCGCCGACGCTCTGCGCGGCCGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003462 Ornithine cyclodeaminase/mu-crystallin (Family)
[1-295] 2.10000267834031e-21 PIRSF001439
PIRSF001439   CryM
[10-284] 6.60000000000002e-70 PF02423
PF02423   OCD_Mu_crystall
IPR016040 NAD(P)-binding domain (Domain)
[135-288] 5.49999999999995e-40 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Virg_00390 Virg_00390   Virg_00410 Virg_00410
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