Virg_00380 Virg_00380   Virg_00400 Virg_00400

Virg_00390 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction111,286 / 109,631 / - [in whole cluster]
3,522 / 1,867 / - [in contig]
Locationcomplement(109631..111286) [in whole cluster]
complement(1867..3522) [in contig]
TypeCDS
Length1,656 bp (551 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase adenylation domain protein
Product (GenBank)3-hydroxypicolinic acid:AMP ligase
Gene
Gene (GenBank)visB
EC number
Keyword
  • virginiamycin S
  • 3-hydroxypicolinic acid
Note
Note (GenBank)
  • VisB
Reference
ACC
PmId
[11943483] Characterization of virginiamycin S biosynthetic genes from Streptomyces virginiae. (Gene. , 2002)
comment
virginiamycin S biosynthetic genesの報告。

visB: 3-hydroxypicolinic acid:AMP ligase

シーケンス解析、RNAの発現解析、転写開始点の決定を行っている。VisBは相同性から機能を同定しているのみ。

visAとvisBはoperonを形成しており、Virginiamycin Sの3-hydroxypicolinic acid moietyの供給に関与すると示唆。
Related Reference
ACC
Q8GA86
PmId
[11112781] The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin. (J Biol Chem. , 2001)
[12221282] Crystal structure of DhbE, an archetype for aryl acid activating domains of modular nonribosomal peptide synthetases. (Proc Natl Acad Sci U S A. , 2002)
comment
Bacillus subtilis(ATCC 21332)
DHB-AMP-ligase DhbE_dhbE

---
[PMID: 11112781](2001)
鉄欠乏に応答して細胞外の鉄を獲得するために産生されるcatecholic siderophore bacillibactinの生合成についての報告。

異種性発現、精製して得たrecombinant protein DhbEの基質特異性がATP-PPi exchange reactionで確認され、2,3-dihydroxybenzoate(DHB)とsalicylateに高く特異的であることがわかった。

---
[PMID: 12221282](2002)
bacillibactinはdhbオペロンでコードされるNRPSによって合成される。そのうちのstand-alone adenylation (A) domain DhbEの結晶構造解析している。

DhbEはbacillibactin合成で最初のステップであるaryl acid 2,3-dihydroxybenzoate (DHB)の活性化を担う。

この構造解析結果とAA配列alignmentsから基質特異性について考察している。

close this sectionPKS/NRPS Module

S0
A53..465

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase adenylation domain protein [3-hydroxypicolinic acid:AMP ligase]
MLDGCVPWPPDAAERYREAGYWRGETLGALLRGWAERYGDRVALVGDDGTRVTYRELDMW
CDRLAAGFAGQGVAPRERVLVQLPNTPSFVAVCFALFRLGAVPVFALPSYAVRSCGTWRS
CPVRSRWWIPDEVRGFDHRALVRDLLPELPRVARVFVAGDPGGEEDGLVALAALEQDPVA
LPEPDASDVAFFLLSGGTTALPKMIPRTHDDYAYQTRITAGICELGEDTVYLAVLPVEFN
FPWGCPGIIGVLGVGGRVVFAPDPTPQTCFPLIERERVTMTSVIPTIVHLWLDAAGRTGH
DLSSLDVVQVGSSKLHDEVAARIEPELGVRLQQVFGMAEGLLTFTRYDDPRQVVLTTQGR
PVSPADEIRVVDGDDREVPDGTVGELLTRGPYTLRGYYRAPEHNASAFTADGFYRSGDLV
RRTPTGEIVVEGRVKDVVIRGGDKVSATEVEGHLTAHPGVQQAAVVAKPDRVLGEKICAY
VVADRDGPAPTLPGVRRLLRARGLADFKLPDRLEVVEVFPLTGLNKVDKKVLAARAGLLH
SGGGAGRGDAR
selected fasta
>non-ribosomal peptide synthetase adenylation domain protein [3-hydroxypicolinic acid:AMP ligase]
ATGCTGGATGGATGCGTGCCGTGGCCCCCTGATGCCGCCGAGCGCTACCGCGAGGCCGGG
TACTGGCGCGGTGAGACGCTCGGTGCCCTGCTGCGCGGTTGGGCCGAGAGGTACGGGGAC
CGGGTCGCCCTGGTGGGTGACGACGGCACCCGGGTGACGTACCGCGAACTGGACATGTGG
TGCGACCGGCTGGCCGCCGGTTTTGCCGGGCAGGGTGTGGCGCCGCGCGAGCGCGTGCTG
GTGCAGCTGCCGAACACCCCGTCGTTCGTGGCGGTCTGTTTCGCCCTGTTCCGGCTGGGC
GCGGTGCCGGTTTTCGCGCTGCCGTCGTACGCAGTGCGGAGCTGCGGCACCTGGCGGAGC
TGTCCGGTGCGGTCGCGATGGTGGATCCCGGACGAGGTCAGGGGGTTCGACCACCGTGCG
CTCGTGCGCGACCTGCTGCCGGAGCTCCCGCGCGTGGCCCGGGTCTTCGTGGCGGGCGAT
CCGGGCGGAGAGGAGGACGGCCTGGTCGCGCTGGCGGCCCTGGAGCAGGATCCGGTCGCA
CTGCCCGAGCCGGACGCCTCCGACGTCGCGTTCTTCCTGTTGTCCGGCGGTACGACCGCG
CTGCCGAAGATGATCCCCCGCACCCACGACGACTACGCGTACCAGACCCGGATCACCGCC
GGGATCTGCGAACTCGGCGAGGACACCGTCTACCTGGCGGTGCTGCCGGTGGAGTTCAAC
TTCCCGTGGGGTTGTCCCGGCATCATCGGCGTACTGGGCGTCGGCGGCCGGGTGGTGTTC
GCGCCGGACCCGACCCCGCAGACCTGCTTCCCGCTGATCGAGCGGGAGCGGGTCACCATG
ACCTCGGTCATCCCGACCATCGTGCACCTGTGGCTGGACGCGGCCGGGCGCACCGGCCAC
GACCTGTCCAGCCTGGACGTCGTACAGGTAGGCAGTTCCAAGCTGCACGACGAGGTCGCC
GCCCGGATCGAGCCCGAGCTCGGGGTGCGGCTCCAGCAGGTGTTCGGCATGGCGGAGGGG
CTGCTGACCTTCACCCGCTACGACGACCCGCGGCAGGTCGTCCTGACCACCCAGGGCCGG
CCGGTGTCGCCGGCCGACGAGATCCGGGTGGTGGACGGCGACGACCGCGAGGTGCCGGAC
GGTACGGTCGGCGAGCTGCTGACCCGGGGCCCGTACACGCTGCGCGGCTACTACCGTGCT
CCGGAGCACAACGCCTCGGCATTCACCGCGGACGGGTTCTACCGCAGCGGTGACCTGGTC
CGCAGGACCCCCACCGGTGAGATCGTGGTCGAGGGCCGGGTCAAGGACGTGGTGATCCGC
GGCGGCGACAAGGTGTCCGCGACGGAGGTCGAGGGCCATCTGACGGCGCACCCGGGCGTG
CAGCAGGCCGCGGTGGTCGCGAAGCCCGACCGCGTACTGGGCGAAAAGATCTGCGCCTAC
GTGGTTGCCGACCGGGACGGGCCGGCGCCCACTCTGCCGGGAGTGCGCCGGCTCCTGCGC
GCCCGCGGCCTGGCCGACTTCAAACTGCCGGACCGACTGGAGGTGGTGGAGGTCTTCCCG
CTGACCGGTCTGAACAAGGTGGACAAGAAGGTCCTCGCGGCCCGGGCCGGGCTCCTGCAC
AGTGGTGGCGGTGCGGGCCGAGGAGATGCGCGGTGA
[0] A53..465
[0] A157..1395

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[53-465] 5.90000000000008e-81 PF00501
PF00501   AMP-binding
SignalP
[1-35] 0.063 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Virg_00380 Virg_00380   Virg_00400 Virg_00400
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