Virg_00380 : CDS information

Location

Organism	Streptomyces virginiae
Strain	MAFF 10-06014
Entry name	Virginiamycin
Contig	AB072568
Start / Stop / Direction	109,634 / 108,375 / - [in whole cluster] 1,870 / 611 / - [in contig]
Location	complement(108375..109634) [in whole cluster] complement(611..1870) [in contig]
Type	CDS
Length	1,260 bp (419 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	L-lysine C-2 aminotransferase
Product (GenBank)	L-lysin 2-aminotransferase
Gene
Gene (GenBank)	visA
EC number	2.6.1.-
Keyword	virginiamycin S 3-hydroxypicolinic acid
Note
Note (GenBank)	VisA
Reference	ACC Q8RR24 PmId [11943483] Characterization of virginiamycin S biosynthetic genes from Streptomyces virginiae. (Gene. , 2002) [12169606] Identification by heterologous expression and gene disruption of VisA as L-lysine 2-aminotransferase essential for virginiamycin S biosynthesis in Streptomyces virginiae. (J Bacteriol. , 2002) comment [PMID:11943483] virginiamycin S(VS) biosynthetic genesの報告。シーケンス解析、RNAの発現解析、転写開始点の決定を行っている。 pyridoxal-phosphate binding site: Lys-190 [PMID:12169606] VisAの機能解析報告。精製したrVisAは best amino acceptorとして2-oxohexanoateと一緒に、明らかにL-lysine 2-aminotransferase activityを示した。 VisAはL-lysineをdelta1-piperidine 2-carboxylic acidへ触媒する。欠損株の作製により、VisAは一次代謝や形態形成には関係せず、VS生合成に必須であることが示された。また、VS生合成のスターター分子である3-hydroxypicolinic acid (3-HPA)を外部添加すると、機能が回復することからvisAがVS biosynthetic pathwayの最初の分子でL-lysineから3-HPAを供給している。

Sequence

>L-lysine C-2 aminotransferase [L-lysin 2-aminotransferase]
MTAAQRYLVPFQRRGSIVGQADLAALASVVHSAEPLSAGVWRERFEQRFREVTGARHALS
VTSGTVALELAVRLLDLQPGDEVIATPQTFQATVQPLLDHDVTVRFCDVDPDTLNMDPQV
LDSLITDRTKAILLVHYGGLPADMAAILARARRAGVVVVEDCAHGWGASYQGDSPGALGD
IGCFSFHSTKNITTLGEGGMITLNRADWAERVERLRGNEVDGVFAPVPGFADDEPALLPW
MRFSADVYRRQAVGIRGAGTNATMSEAAAAVGLVQLDALERFTERRRWIAARLDAAVSAF
PRHPPPTHAGGSTHAYHLYTFFVTAGREGARDSCAPLDRRGVEVQLRYFPLHLLPEVAAA
RPRSGRVPQAERVWFDEHLNLPCHPGLTDEQVDHLVESVTAAMHEATALARTGRPLAVR

>L-lysine C-2 aminotransferase [L-lysin 2-aminotransferase]
GTGACGGCGGCGCAGCGCTACCTGGTGCCGTTCCAGCGCCGGGGGAGCATCGTGGGCCAG
GCCGACCTGGCGGCGCTCGCCTCGGTCGTGCACTCGGCGGAGCCGCTGTCGGCGGGGGTG
TGGCGCGAGCGGTTCGAGCAGCGCTTCCGGGAGGTCACCGGGGCCCGCCACGCGCTGTCG
GTGACCAGCGGGACGGTGGCTCTGGAGCTGGCCGTCCGGCTGCTCGACCTGCAGCCCGGC
GACGAGGTGATCGCCACCCCGCAGACGTTCCAGGCGACGGTGCAGCCGTTGCTCGACCAC
GACGTCACCGTGCGGTTCTGCGACGTCGACCCGGACACCCTGAACATGGACCCGCAGGTC
CTGGATTCGCTGATCACCGACCGGACGAAGGCCATCCTGCTGGTGCACTACGGCGGCCTT
CCGGCGGACATGGCGGCCATCCTGGCCCGGGCCCGCCGTGCGGGCGTCGTGGTGGTGGAG
GACTGCGCGCACGGCTGGGGCGCTTCCTACCAGGGGGACAGCCCCGGCGCGCTCGGCGAC
ATCGGGTGCTTCAGCTTCCACTCGACGAAGAACATCACCACGCTCGGCGAGGGGGGCATG
ATCACGCTGAACCGGGCCGACTGGGCCGAGCGGGTCGAGCGGCTGCGGGGCAACGAGGTG
GACGGGGTGTTCGCCCCGGTGCCCGGCTTCGCGGACGACGAGCCCGCGCTGCTGCCGTGG
ATGCGCTTCTCCGCCGACGTCTACCGCCGCCAGGCCGTCGGTATCCGGGGAGCGGGCACC
AACGCCACCATGTCCGAGGCCGCCGCGGCGGTCGGGCTGGTCCAGCTGGATGCGCTGGAG
CGGTTCACCGAGCGCCGTCGGTGGATCGCGGCCCGGCTGGACGCCGCCGTCTCGGCGTTT
CCCCGGCACCCGCCTCCAACGCACGCCGGAGGCAGCACCCACGCCTACCACCTGTACACG
TTCTTCGTCACGGCCGGGCGTGAGGGTGCGCGAGATTCGTGCGCGCCCCTGGACCGCAGG
GGCGTGGAGGTTCAGCTGCGGTACTTCCCGCTGCACCTGCTGCCCGAAGTGGCGGCTGCG
CGGCCACGGTCCGGGCGAGTGCCGCAGGCCGAGCGGGTCTGGTTCGACGAGCACCTGAAC
CTGCCCTGCCACCCGGGGCTCACCGACGAGCAGGTCGACCACCTTGTCGAGTCGGTCACC
GCTGCGATGCACGAGGCCACGGCCCTCGCCAGGACGGGCCGGCCGCTGGCGGTCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family) PF01041 DegT_DnrJ_EryC1 PIRSF000390 PLP_StrS IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain) G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain) G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain) SSF53383 PyrdxlP-dep_Trfase_major
SignalP	Eukaryota
TMHMM	No significant hit

Virg_00370 Virg_00390