Virg_00360 Virg_00360   Virg_00380 Virg_00380

Virg_00370 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction106,735 / 107,478 / + [in whole cluster]
111 / 854 / + [in contig]
Location106735..107478 [in whole cluster]
111..854 [in contig]
TypeCDS
Length744 bp (247 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductTetR family transcriptional regulator
Product (GenBank)VarR
Gene
Gene (GenBank)varR
EC number
Keyword
  • virginiamycin S
Note
Note (GenBank)
Reference
ACC
PmId
[11222601] Identification of the varR gene as a transcriptional regulator of virginiamycin S resistance in Streptomyces virginiae. (J Bacteriol. , 2001)
comment
varR機能解析の報告

VarR: transcriptional protein(TetR-type repressor)

大腸菌にて発現させたリコンビナント酵素との比較により機能解析している。

varRは virginiamycin S生産が開始するまでvarSのプロモーター領域に結合することによりvarS-varR operonの転写を抑制し、virginiamycin S生産が開始されるとvarSのプロモーター領域からvarRの解離が起こり抑制が軽減されると推測した。

varRの転写はvarSとcotranscribedに行われ(varS-varR operon)、Virginiamycin Sはこのbicistronic transcriptionのinducerとして働く。
Related Reference
ACC
Q9F0Y2
PmId
[11050092] A transcriptional regulator of a pristinamycin resistance gene in Streptomyces coelicolor. (J Biol Chem. , 2001)
comment
Streptomyces coelicolor
Pip

pristinamycin-induced transcriptional regulator.
精製し、promoterとのaffinity等確認している。

close this sectionSequence

selected fasta
>TetR family transcriptional regulator [VarR]
MAAKRASQPRSSVWLTPEPTTRGRRGPERGSGAGSLDRERIVAAAVRLLDEQGDAKFTMR
VLATELGVTPMSVYWYIANKDDLMELALDAVAAEIKLPDPAAGLDWREDLRALALSWRRT
MVSHPWAIRSYAEYLNIGPHSMRFSACAQAVVARSPLPEAERPAALSAVFQYVYGFTSME
SRWLEYGKEVGRTADEFLEEVAGSIAQAPEIEAGGGLMERRAGRSLDEMRDSDFDRALDW
LIAGMCD
selected fasta
>TetR family transcriptional regulator [VarR]
GTGGCCGCCAAGCGCGCCTCGCAACCGAGAAGCAGCGTGTGGCTCACTCCGGAGCCGACG
ACCCGCGGCCGCCGGGGTCCCGAACGCGGTTCGGGAGCGGGCAGCCTGGACCGGGAAAGG
ATCGTCGCCGCCGCGGTGCGACTGCTCGACGAACAGGGCGACGCGAAGTTCACGATGCGC
GTGCTGGCCACGGAACTGGGGGTCACCCCCATGTCGGTCTACTGGTACATCGCGAACAAG
GACGACCTGATGGAGCTGGCCCTCGACGCCGTGGCGGCGGAGATAAAGCTCCCCGACCCG
GCGGCCGGCCTGGACTGGCGGGAGGACCTGCGTGCGCTGGCGCTCTCCTGGCGGCGCACC
ATGGTCTCCCACCCGTGGGCCATCCGCTCGTACGCCGAGTACCTCAACATCGGCCCGCAC
AGCATGCGGTTCTCCGCCTGCGCCCAGGCGGTCGTCGCCCGGTCGCCGCTGCCCGAGGCC
GAACGACCCGCCGCGCTGTCGGCCGTGTTCCAGTACGTGTACGGCTTCACCTCGATGGAG
AGCCGCTGGCTGGAGTACGGCAAGGAAGTGGGCCGTACGGCCGACGAGTTCCTGGAGGAG
GTCGCCGGCAGCATCGCGCAGGCCCCCGAGATCGAGGCCGGCGGCGGACTGATGGAACGC
CGCGCCGGACGCAGCCTCGACGAGATGCGCGACAGCGACTTCGACCGCGCCCTCGACTGG
CTGATCGCCGGCATGTGTGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001647 DNA-binding HTH domain, TetR-type (Domain)
[35-95] PS50977
PS50977   HTH_TETR_2
[41-85] 2e-11 PF00440
PF00440   TetR_N
[41-54] 2.59999946718995e-06 PR00455 [62-85] 2.59999946718995e-06 PR00455
PR00455   HTHTETR
IPR004111 Tetracycline transcriptional regulator, TetR, C-terminal (Domain)
[100-245] 2.2e-11 PF02909
PF02909   TetR_C
IPR009057 Homeodomain-like (Domain)
[28-91] 1.5e-21 G3DSA:1.10.10.60
G3DSA:1.10.10.60   Homeodomain-rel
[36-95] 9.40001006865064e-13 SSF46689
SSF46689   Homeodomain_like
IPR011075 Tetracycline transcriptional regulator, TetR-related, C-terminal (Domain)
[104-245] 1.50000306971104e-24 SSF48498
SSF48498   TetR_like_C
IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain)
[92-245] 6.3e-24 G3DSA:1.10.357.10
G3DSA:1.10.357.10   TetR_C
SignalP
[1-23] 0.134 Signal
Eukaryota   
TMHMM No significant hit
Virg_00360 Virg_00360   Virg_00380 Virg_00380
Page top