Salino_00960 Salino_00960   Salino_00980 Salino_00980

Salino_00970 : CDS information

close this sectionLocation

Organism
StrainDSM 41398
Entry nameSalinomycin
Contig
Start / Stop / Direction158,484 / 163,418 / + [in whole cluster]
158,484 / 163,418 / + [in contig]
Location158484..163418 [in whole cluster]
158484..163418 [in contig]
TypeCDS
Length4,935 bp (1,644 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)modular polyketide synthase
Gene
Gene (GenBank)salAVII
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

salAVII (1644aa): polyketide synthase
KS, AT, KR, ACP
Related Reference
ACC
H6D575
NITE
Salino3_00190
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
配列は[H1ZZT9]と全く同じ。株違い。

polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

SlnA7 (1,644aa) : PKS
Module 11 : KS-ATp-KR-ACP

close this sectionPKS/NRPS Module

11 methylmalonyl-CoA
KS36..411
AT562..880
KR1202..1381
ACP1490..1565

close this sectionSequence

selected fasta
>polyketide synthase [modular polyketide synthase]
MSTSNEAKLREYLKRVTTDLSQTRQQLREVENAGHEPLAIVSMACRFPGGVTDPEGFWQL
IASGTDAISTFPTDRGWDVENLYDPDPDKPGTTYVREGGFLDDATLFDPELFGISPREAA
AMDPQQRLFLETAWETLERGGFDPLSLKSTQTGVFVGAVASDYASRLDALPEGTEGYIGT
GTMSSVLSGRIAYTFGLAGPAVTVDTACSSGLVALHLAVQALRQGECDLALAAGVAVMAS
PAAFVEFSRQRALAPDGRCKAFGAGADGTTWSEGAGVLLVERLSDAQRNGHQVLAVIRGS
ALNQDGASNGLTAPSEPAQQEVIRRALANARLTAAQVDAVEAHGTGTTLGDPIEAQALLA
TYGKEKSAEAPLWLGSVKSNIGHGGPVAGIAGVIKMVQALRHGQLPRTLHAEESSPHIDW
SSGAVKLLTEARDWAPGADPRRAGVSSFGVSGTNAHVILEEAPAAEEPADTAAEDAGGPA
LWTLSGRTEAALRAQAARLLDHVREGADLASTGWSLATTRTAHPVRAAVAAPDHASFLSG
LQALADGDDLGSQAVPGADRPVFVFPGQGSQWPGMAVELLDSSPVFAARLQECAEALAPY
VDWSLLDVLRQADGAPGFDRVDVVQPALWAVMVSLAELWRANGIRPAAVLGHSQGEIAAA
AVSGALSLGDAAKVSALRAKALLALAGKGGMVSVAAPAAEVTTRIAAWGERLSLASVNGP
RSTVVSGEPGALDELLAACERDGVRARRINVDYASHSAQVESIREEVLGALAGITPRTAE
VPFFSTVTALPATGTELDAEYWFTNLRTTVRFEEGVRALLASGHGLFVESSAHPVLTIGV
QETIDELGSSAVTLGTLRREEGSPLRFLSSLGEAWAHGAAPDWAAVYRDRPVRRVPLPTY
AFQHRSYWLEDESAAAASGTAPGGPVDEVDAAFWAAVENGDLSALDGLDLAADQPIGSVL
PALTDWRRTRREKSVMDSWRYRTVWTVAETAEPALTGTWLIIEPEGQAEDLAEAAAQQLK
EHGAQSIRIPVEIDAADRYTLAQQIHETLTEGTEITGVLSLLSLDERPHPEYEALPLGLA
GTVTLVQAVLQVRELAEFDAPVWSLTQGAVSIGDTDAVTSPVQAAVWGLARVLVQEHPRL
WGGIVDLPQQVDEKAWTRLARVLTEPGDEDQLAIRSWDILVRRLVRAPLGDTAPAREWKP
EGTALVTGGTGGLGAHTARWLARSGAEHLLLVSRSGADAPGAAGLTAELEELGARVTLAA
ADVSDREALAGVLAAIPAETPLTSVFHTAAVLDDGMVDTLTPDRLERTFQVKARGAQHLA
ELTADAGLTAFVLFSSFGASYGSAGLGNYTPGNTFLDALAEQRRAQGLPATSVVWGTWEG
SGMADNGVGERARTQGVWELPPEQATAALQQALDHEETNPVLIDIRWDSFSLGTNTERNS
PLFEMVPEAAAKVAAAQAEAESAAEESAGAPEALLARLQAQPPSEQERILLELVRKQAAS
VLGHGSTGEGALAAVGPDRPFRELGFDSLSAVELRNRLGAATGLSLPSTLVFDHPTPAEL
AAELRTELLGESGGVEPVLDEIGRLEAVLGSAPEDPEARAAITGRLQALLSTWTGKGGNQ
GESDVDAASDDELFGLLDDELETK
selected fasta
>polyketide synthase [modular polyketide synthase]
ATGTCGACGAGCAACGAAGCGAAGCTCCGCGAGTACCTCAAGCGCGTCACCACCGACCTC
AGCCAGACCCGACAGCAGCTGCGCGAGGTGGAGAACGCCGGCCACGAGCCGCTCGCCATC
GTCTCGATGGCCTGCCGCTTCCCCGGCGGCGTCACCGACCCCGAGGGCTTCTGGCAGCTG
ATCGCCTCCGGCACCGACGCGATCAGCACCTTCCCCACCGACCGCGGCTGGGACGTGGAG
AACCTCTACGACCCGGACCCCGACAAGCCGGGCACCACCTACGTCCGCGAGGGCGGCTTC
CTCGACGACGCCACCCTCTTCGACCCCGAGCTGTTCGGCATCTCGCCCCGCGAGGCCGCC
GCGATGGACCCGCAGCAGCGGCTCTTCCTGGAGACCGCCTGGGAGACCCTGGAGCGCGGC
GGCTTCGACCCCCTGTCGCTGAAGTCGACGCAGACCGGGGTCTTCGTGGGCGCCGTCGCC
TCCGACTACGCCTCCCGCCTGGACGCGCTCCCGGAGGGCACCGAGGGCTACATCGGCACC
GGCACCATGTCCTCCGTGCTGTCCGGCCGCATCGCCTACACCTTCGGCCTGGCCGGTCCC
GCCGTCACCGTGGACACCGCCTGCTCCTCCGGTCTGGTCGCCCTGCACCTGGCGGTCCAG
GCGCTGCGCCAGGGCGAGTGCGACCTGGCGCTCGCGGCCGGGGTCGCCGTGATGGCGAGC
CCCGCCGCCTTCGTCGAGTTCAGCCGCCAGCGGGCGCTGGCCCCCGACGGCCGCTGCAAG
GCCTTCGGCGCGGGCGCCGACGGCACCACCTGGTCCGAGGGCGCGGGCGTCCTCCTCGTG
GAGCGCCTCTCCGACGCGCAGCGCAACGGGCACCAGGTCCTCGCCGTGATCCGCGGCTCC
GCCCTCAACCAGGACGGCGCCAGCAACGGACTCACCGCCCCCAGCGAGCCCGCCCAGCAG
GAGGTCATCCGCCGCGCCCTCGCCAACGCCCGGCTCACCGCGGCCCAGGTCGACGCGGTC
GAGGCGCACGGCACCGGCACCACCCTCGGCGACCCCATCGAGGCGCAGGCCCTGCTCGCC
ACGTACGGCAAGGAGAAGAGCGCCGAGGCGCCGCTGTGGCTCGGCTCGGTGAAGTCGAAC
ATCGGCCACGGCGGACCGGTCGCGGGCATCGCCGGGGTCATCAAGATGGTGCAGGCGCTG
CGGCACGGACAGCTGCCGCGCACCCTGCACGCCGAGGAGAGCTCCCCGCACATCGACTGG
TCCAGCGGCGCGGTCAAGCTGCTCACCGAGGCGCGGGACTGGGCCCCGGGCGCCGATCCG
CGCCGGGCCGGTGTCTCCTCCTTCGGCGTCAGCGGCACCAACGCCCACGTCATCCTCGAA
GAGGCCCCGGCCGCCGAGGAGCCGGCGGACACCGCGGCCGAGGACGCGGGCGGCCCCGCC
CTGTGGACCCTCTCCGGGCGCACCGAGGCCGCGCTGCGCGCCCAGGCCGCCCGGCTGCTC
GACCACGTCCGCGAGGGCGCCGACCTCGCCTCCACCGGCTGGTCGCTGGCCACCACCCGT
ACCGCGCACCCGGTCAGGGCCGCGGTCGCGGCGCCGGACCACGCCTCCTTCCTCTCCGGT
CTGCAGGCCCTCGCCGACGGCGACGACCTCGGCAGCCAGGCCGTCCCCGGCGCCGACCGC
CCCGTCTTCGTCTTCCCCGGCCAGGGTTCGCAGTGGCCGGGCATGGCGGTCGAACTCCTG
GACTCCTCGCCGGTGTTCGCGGCGCGGTTGCAGGAGTGCGCCGAGGCGCTGGCCCCGTAC
GTGGACTGGTCGCTGCTGGATGTGCTGCGGCAGGCGGACGGCGCCCCGGGCTTCGACCGG
GTGGATGTCGTGCAGCCCGCGCTGTGGGCCGTGATGGTCTCCCTCGCCGAACTCTGGCGC
GCCAACGGCATCCGCCCGGCGGCGGTGCTCGGCCACTCCCAGGGCGAGATCGCCGCCGCG
GCCGTCTCCGGTGCGCTGTCGCTCGGCGACGCCGCGAAGGTCTCGGCGCTGCGGGCCAAG
GCGCTGCTCGCGCTCGCGGGCAAGGGCGGCATGGTCTCGGTCGCGGCCCCCGCCGCCGAG
GTCACCACCCGTATCGCGGCCTGGGGCGAACGCCTCTCGCTCGCCTCGGTCAACGGCCCC
CGCTCGACCGTGGTCTCCGGTGAACCGGGCGCCCTGGACGAGCTGTTGGCCGCCTGCGAG
CGGGACGGGGTGCGCGCCCGCCGGATCAACGTCGACTACGCCTCGCACTCGGCCCAGGTG
GAGTCCATCCGCGAGGAGGTGCTCGGCGCCCTCGCCGGGATCACCCCGCGCACGGCCGAG
GTGCCCTTCTTCTCCACGGTCACCGCCCTGCCGGCGACGGGCACCGAGCTGGACGCCGAG
TACTGGTTCACCAACCTGCGCACCACCGTCCGCTTCGAGGAGGGCGTCCGCGCCCTGCTC
GCCTCCGGGCACGGCCTCTTCGTGGAGTCCAGCGCCCACCCGGTGCTCACCATCGGGGTG
CAGGAGACCATCGACGAGCTCGGCTCCTCGGCGGTCACCCTCGGCACCCTGCGCCGCGAG
GAGGGCAGCCCGCTGCGCTTCCTCAGCTCGCTCGGCGAGGCCTGGGCGCACGGCGCCGCC
CCCGACTGGGCGGCCGTCTACCGCGACCGCCCGGTACGCCGCGTCCCGCTGCCCACCTAC
GCCTTCCAGCACCGCAGTTACTGGCTGGAGGACGAGAGCGCGGCCGCCGCGAGCGGCACC
GCCCCCGGCGGCCCTGTCGACGAGGTGGACGCCGCCTTCTGGGCCGCCGTCGAGAACGGC
GACCTCTCCGCGCTCGACGGCCTCGACCTCGCCGCCGACCAGCCGATCGGCTCCGTACTG
CCCGCCCTGACCGACTGGCGGCGTACCCGAAGGGAAAAGTCCGTCATGGACTCCTGGCGC
TACCGCACTGTGTGGACCGTCGCCGAGACCGCCGAGCCCGCCCTCACCGGCACCTGGCTC
ATCATCGAACCCGAGGGGCAGGCCGAGGACCTCGCCGAGGCCGCGGCGCAGCAGCTCAAG
GAGCACGGCGCCCAGAGCATCCGCATCCCGGTCGAGATCGACGCCGCGGACCGCTACACC
CTGGCCCAGCAGATCCACGAGACGCTGACCGAGGGCACCGAGATCACCGGTGTGCTCTCG
CTGCTCTCCCTCGACGAGCGCCCGCACCCCGAGTACGAGGCGCTGCCGCTCGGCCTCGCG
GGCACGGTCACCCTGGTGCAGGCCGTCCTCCAGGTCCGCGAACTCGCCGAGTTCGACGCG
CCGGTGTGGTCGCTGACCCAGGGCGCCGTCTCGATCGGCGACACGGACGCCGTCACCAGC
CCGGTGCAGGCCGCCGTCTGGGGCCTGGCCCGCGTCCTGGTGCAGGAGCACCCGCGGCTG
TGGGGCGGCATCGTCGACCTGCCGCAGCAGGTGGACGAGAAGGCCTGGACCCGGCTCGCC
CGGGTGCTCACCGAACCCGGCGACGAGGACCAACTCGCCATCCGCTCCTGGGACATCCTG
GTCCGCAGGCTGGTCCGCGCGCCCCTCGGGGACACCGCCCCGGCCCGCGAGTGGAAGCCT
GAGGGCACCGCCCTGGTCACCGGCGGCACCGGCGGCCTCGGCGCCCACACCGCCCGCTGG
CTGGCCCGCAGCGGCGCCGAGCACCTGCTCCTGGTCAGCCGCAGCGGCGCGGACGCCCCC
GGCGCCGCCGGACTCACCGCCGAACTCGAAGAGCTGGGCGCCCGGGTGACCCTCGCCGCC
GCCGACGTCTCGGACCGCGAGGCGCTCGCCGGGGTGCTCGCGGCGATCCCCGCCGAGACG
CCGCTGACCTCCGTCTTCCACACCGCCGCCGTCCTCGACGACGGCATGGTCGACACCCTC
ACCCCGGACCGCCTGGAGCGCACCTTCCAGGTCAAGGCGCGCGGCGCCCAGCACCTCGCC
GAACTCACCGCCGACGCCGGTCTGACCGCCTTCGTGCTCTTCTCCTCCTTCGGCGCCTCC
TACGGCTCCGCGGGCCTCGGCAACTACACGCCCGGCAACACCTTCCTGGACGCCCTCGCC
GAGCAGCGGCGCGCCCAGGGGCTGCCCGCCACCTCGGTGGTCTGGGGCACCTGGGAGGGC
AGCGGCATGGCCGACAACGGCGTCGGCGAGCGGGCCCGCACCCAGGGCGTCTGGGAGCTG
CCGCCCGAGCAGGCCACCGCCGCGCTCCAGCAGGCCCTGGACCACGAGGAGACCAACCCG
GTCCTCATCGACATCCGCTGGGACAGCTTCTCGCTCGGCACCAACACCGAGCGGAACAGC
CCGCTGTTCGAGATGGTGCCGGAGGCCGCCGCCAAGGTGGCCGCCGCGCAGGCCGAGGCC
GAGTCCGCCGCCGAGGAGAGCGCCGGCGCCCCCGAGGCCCTGCTCGCCCGGCTGCAGGCG
CAGCCGCCGTCCGAGCAGGAGCGGATCCTGCTCGAACTGGTCCGCAAGCAGGCCGCCTCC
GTCCTCGGCCACGGCAGCACGGGCGAGGGCGCCCTCGCCGCCGTCGGACCCGACCGGCCC
TTCAGGGAACTCGGCTTCGACTCGCTGAGCGCCGTCGAACTGCGCAACCGGCTCGGCGCC
GCCACCGGCCTCAGCCTGCCCTCCACCCTCGTCTTCGACCACCCGACGCCCGCCGAACTC
GCCGCCGAGCTCCGGACCGAACTCCTCGGCGAGAGCGGCGGGGTGGAGCCGGTGCTCGAC
GAGATCGGGCGCCTGGAAGCCGTACTGGGCTCCGCCCCGGAGGACCCGGAGGCCCGCGCG
GCCATCACCGGCCGCCTCCAGGCACTGCTGTCCACCTGGACCGGCAAGGGCGGGAACCAG
GGCGAGAGCGATGTGGACGCGGCCTCCGATGACGAGCTGTTCGGCCTGCTCGACGACGAA
CTCGAAACGAAATAA
[11] KS36..411
[11] AT562..880
[11] methylmalonyl-CoA753..757
[11] KR1202..1381
[11] ACP1490..1565
[11] KS106..1233
[11] AT1684..2640
[11] methylmalonyl-CoA2257..2271
[11] KR3604..4143
[11] ACP4468..4695

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[752-869] 4.1e-70 G3DSA:3.40.366.10
G3DSA:3.40.366.10   no description
IPR006162 Phosphopantetheine attachment site (PTM)
[1523-1538] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1513-1564] 2.1e-12 PF00550
PF00550   PP-binding
[1490-1565] 20.435 PS50075
PS50075   ACP_DOMAIN
[1482-1582] 5.2e-22 SSF47336
SSF47336   ACP-like
[1490-1568] 5.6e-19 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
IPR013968 Polyketide synthase, KR (Domain)
[1202-1380] 5e-59 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[36-286] 5.7e-97 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[294-411] 2.8e-44 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[562-880] 1.1e-107 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[4-29] 1.6e-12 PF08990
PF08990   Docking
[2-36] 8e-09 SSF101173
SSF101173   Docking domain B of the erythromycin polyketide synthase (DEBS)
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[560-862] 2.3e-65 SSF52151
SSF52151   FabD/lysophospholipase-like
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[687-752] 1.1e-18 SSF55048
SSF55048   Probable ACP-binding domain of malonyl-CoA ACP transacylase
IPR016038 Thiolase-like, subgroup (Domain)
[39-298] 3.9e-90 G3DSA:3.40.47.10 [299-464] 4e-61 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
[28-410] 4.5e-99 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1202-1418] 3.4e-34 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[199-215] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[564-861] 3.1e-114 SM00827
SM00827   Acyl transferase domain in polyketide syntha
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1491-1568] 8e-30 SM00823
SM00823   Phosphopantetheine attachment site
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[38-464] 7.4e-220 SM00825
SM00825   Beta-ketoacyl synthase
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1202-1381] 2.6e-50 SM00822
SM00822   no description
SignalP No significant hit
TMHMM No significant hit
Salino_00960 Salino_00960   Salino_00980 Salino_00980
Page top