Salino_01110 Salino_01110   Salino_01130 Salino_01130

Salino_01120 : CDS information

close this sectionLocation

Organism
StrainDSM 41398
Entry nameSalinomycin
Contig
Start / Stop / Direction195,365 / 197,158 / + [in whole cluster]
195,365 / 197,158 / + [in contig]
Location195365..197158 [in whole cluster]
195365..197158 [in contig]
TypeCDS
Length1,794 bp (597 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)putative non-ribosomal peptide synthetase domains C, PCP
Gene
Gene (GenBank)salX
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

salX (597aa): non-ribosomal peptide synthase
C, PCP

monensinなどのpolyether生合成では、discrete KS (KSX)が最終moduleの終末ACPに特異的に結合し、完全長polyketide鎖をdiscrete ACP (ACPX)へ移し、そこでoxidative cyclisationが起こることが報告されている。

sal clusterにおけるKSX候補は、PKSのC末ACPにfusionしている(SalAIX)。
ACPX候補はSalXである。SalXのN末に見られるNRPSの典型的なC domainは、active site His138がArgによって置換されており縮合活性がないようだ。
Related Reference
ACC
H6D590
NITE
Salino3_00340
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
Streptomyces albus XM211_orf13
Putative peptide synthetase

position 558までは[H1ZZV4]と配列が完全一致。
position 559以降は相同性なし、C末余る。

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[PMID: 22156425]
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いて、Streptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

Orf13 (862aa) : Peptide synthetase

orf13を置換により不活化してもsalinomycin生産性に変化なし。

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase domains C, PCP]
MIPLSHAQNRLWPADRLGTGHEACHLPLVLRHSGPLDVRALRLALTDLVARHEILRTVFP
ERAGVPYQQILTEAPLPLPVEESGEERLTEDLRRVSAEPFDLRTGIPLRAHLFSLPGGSH
VLLVVLHRIAADEDSLSLLLTGLREAYAARREGAPPSWKPLPVQYADFALWQREVLGDLR
DPDSLLSRQLGEWSTALAGLPQRLTLPADRPGPARWESPAGCVRFKVGAALHRDLLDLGR
GSRATLFMVLHAALAALLTSLGAGEDIPVGSPVTGRADEVLEGVVGCFTNTLVIRSDTAD
KPSFRELLARVRERVLFAYAHGDVPFDLLAEEIAPASAAARAPLVQVLLSLRTERAQEPA
GEPEFTREEPARRTAPAELDLAFTQRRAGDGTPQGMTAVLTYAADRFDHPTAQSLAGELH
TLLRTAVREPLPPPGTEELLGPEQRGPFATAEAEQPGSRMRRSRRERLGHRREEVAAAPG
EGPGEDLCAAAAARARPPATPLESSLCRILAEVLAVEEVEPEDDFFELGGDSLLGMRVLS
RVRARLGIELGVCALFDAPTAAGLAEAFTRAEQDAADDDERGDEPPGRQPCGEGPRA
selected fasta
>non-ribosomal peptide synthetase [putative non-ribosomal peptide synthetase domains C, PCP]
GTGATCCCGCTGTCCCATGCCCAGAACCGGCTGTGGCCGGCTGACCGGCTGGGCACCGGC
CACGAGGCCTGCCATCTGCCGCTGGTGCTGCGGCACTCCGGCCCGCTCGACGTGCGCGCC
CTGCGCCTCGCGCTGACCGACCTCGTCGCCCGGCACGAGATCCTCCGCACCGTCTTCCCC
GAACGCGCCGGGGTGCCCTACCAGCAGATCCTCACCGAGGCCCCGCTCCCGCTGCCGGTC
GAGGAGAGCGGCGAGGAGCGGCTGACCGAGGACCTGCGGCGGGTGAGCGCCGAACCCTTC
GACCTGCGGACCGGCATCCCGCTGCGGGCCCACCTGTTCAGCCTCCCCGGCGGCAGCCAC
GTACTCCTGGTGGTGCTGCACCGCATCGCCGCCGACGAGGACTCCCTCTCCCTGCTGCTC
ACCGGGCTCCGCGAGGCGTACGCGGCCCGCCGCGAGGGCGCGCCGCCGTCCTGGAAGCCG
CTGCCGGTGCAGTACGCCGACTTCGCGCTGTGGCAGCGCGAGGTCCTCGGCGATCTGCGC
GACCCGGACAGCCTGCTCAGCCGTCAGCTCGGCGAGTGGAGCACGGCCCTCGCCGGGCTG
CCGCAGCGGCTCACCCTGCCCGCGGACCGGCCGGGGCCCGCCCGGTGGGAGAGCCCGGCG
GGCTGCGTGCGCTTCAAGGTCGGGGCCGCCCTGCACCGGGACCTCCTCGACCTGGGGCGC
GGCAGCCGGGCCACCCTCTTCATGGTGCTGCACGCAGCCCTGGCCGCCCTGCTGACCTCA
CTCGGCGCGGGCGAGGACATCCCGGTCGGCTCCCCCGTGACGGGCCGCGCCGACGAGGTC
CTCGAAGGGGTCGTCGGCTGCTTCACCAACACCCTGGTGATCCGCTCCGACACCGCCGAC
AAGCCGTCCTTCCGCGAACTGCTCGCCCGGGTCCGCGAACGCGTCCTGTTCGCCTACGCC
CACGGCGACGTCCCCTTCGACCTGCTCGCCGAGGAGATCGCCCCGGCCTCGGCCGCCGCC
CGCGCCCCGCTCGTCCAGGTCCTGCTGTCGCTGCGCACCGAACGCGCCCAGGAGCCCGCC
GGGGAACCGGAGTTCACCCGCGAGGAGCCCGCCCGCCGCACCGCCCCGGCCGAACTGGAC
CTCGCCTTCACCCAGCGCCGCGCCGGGGACGGCACCCCGCAGGGCATGACCGCGGTGCTC
ACCTACGCCGCCGACCGCTTCGACCACCCGACCGCCCAGTCCCTCGCGGGCGAGCTGCAC
ACCCTGCTGCGCACCGCCGTACGCGAGCCGCTCCCGCCGCCCGGCACCGAGGAGCTCCTC
GGCCCCGAGCAGCGCGGTCCCTTCGCCACCGCCGAGGCGGAGCAGCCCGGTTCACGGATG
CGCCGCAGCAGACGCGAGCGCCTCGGCCACCGCCGGGAGGAGGTCGCCGCGGCACCGGGG
GAGGGGCCCGGCGAGGACCTCTGCGCCGCGGCGGCGGCCCGGGCCAGGCCCCCGGCGACC
CCGCTGGAGTCGAGCCTGTGCCGGATCCTCGCCGAGGTGCTCGCCGTGGAGGAGGTGGAA
CCCGAGGACGACTTCTTCGAGCTGGGCGGGGACTCCCTGCTCGGCATGCGCGTCCTCAGC
CGGGTCCGCGCCCGGCTCGGCATCGAACTGGGCGTCTGCGCCCTCTTCGACGCCCCGACC
GCCGCCGGTCTCGCCGAGGCCTTCACCCGCGCCGAGCAGGACGCCGCCGACGACGACGAG
CGAGGCGACGAGCCGCCCGGACGGCAGCCGTGCGGGGAGGGCCCCCGCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001242 Condensation domain (Domain)
[1-297] 2.80000000000001e-50 PF00668
PF00668   Condensation
IPR006162 Phosphopantetheine attachment site (PTM)
[527-542] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[506-567] 7.90000000000001e-14 PF00550
PF00550   PP-binding
[492-573] 8.19999999999997e-26 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[495-570] 3.39999972437717e-19 SSF47336
SSF47336   ACP_like
[502-569] PS50075
PS50075   ACP_DOMAIN
SignalP No significant hit
TMHMM No significant hit
Salino_01110 Salino_01110   Salino_01130 Salino_01130
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