Salino_01140 Salino_01140   Salino_01160 Salino_01160

Salino_01150 : CDS information

close this sectionLocation

Organism
StrainDSM 41398
Entry nameSalinomycin
Contig
Start / Stop / Direction198,264 / 198,016 / - [in whole cluster]
198,264 / 198,016 / - [in contig]
Locationcomplement(198016..198264) [in whole cluster]
complement(198016..198264) [in contig]
TypeCDS
Length249 bp (82 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • orf6
Reference
ACC
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

orf6 (73aa) : unknown

詳細記載なし。
Related Reference
ACC
H6D591
NITE
Salino3_00350
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
配列は[H1ZZV7]と全く同じ。株違い。
このORFの周辺は遺伝子編成が少し異なる。

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[PMID: 22156425]
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いて、Streptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

Orf14 (82aa) : Hypothetical protein

orf14-orf17を削除すると、salinomycin産生はwildの45%に低下した。
この結果はSARP family transcriptional regulator gene orf15が削除されたためと考えられている。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MFPVVKVVPVSEFAFGVDLTEGEMRRRAAVVEALGSDWDPVAVLEGERAAHDLLYSGLDA
EQQKTYELLVAAGVLEDRQARP
selected fasta
>hypothetical protein [hypothetical protein]
GTGTTCCCGGTAGTGAAGGTGGTCCCCGTGAGCGAGTTCGCGTTCGGCGTCGACCTGACG
GAGGGCGAGATGCGGCGCCGTGCCGCGGTCGTCGAGGCGCTCGGCTCCGACTGGGACCCG
GTGGCCGTACTGGAGGGCGAGCGCGCCGCCCACGATCTGCTCTACTCCGGTCTCGACGCG
GAGCAGCAGAAGACCTACGAGCTGCTCGTGGCCGCGGGTGTCCTGGAGGACCGGCAGGCC
CGGCCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro No significant hit
SignalP No significant hit
TMHMM No significant hit
Salino_01140 Salino_01140   Salino_01160 Salino_01160
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