Ansam_00180 Ansam_00180   Ansam_00200 Ansam_00200

Ansam_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction34,352 / 35,014 / + [in whole cluster]
48,395 / 47,733 / - [in contig]
Location34352..35014 [in whole cluster]
complement(47733..48395) [in contig]
TypeCDS
Length663 bp (220 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative O-methyltransferase
Product (GenBank)O-methyltransferase
Gene
Gene (GenBank)asm17
EC number2.1.1.-
Keyword
  • methoxymalonyl-ACP
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
[PMID:11996558](2002) abstract
S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6-deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。

また、methyltransferaseをコードするasm17を削除すると、そのhydroxy類似体ではなく6-deoxyerythronolide Bが得られたので、伸長unitのmethylationはその取り込みのために必要である。

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[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm17(167aa) : O-Methyltransferase

asm13-17はオペロンを形成。
稀なpolyketide鎖伸長unit methoxymalonayl-ACPの形成に関連すると提唱されている。
よってAsm17はansamitocin P-3のC-10でのO-methylationに割り当てられる。
Related Reference
ACC
P28034
PmId
[1894629] Molecular cloning, induction and taxonomic distribution of caffeoyl-CoA 3-O-methyltransferase, an enzyme involved in disease resistance. (J Biol Chem. , 1991)
[9037150] Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element. (Plant Mol Biol. , 1997)
comment
Blast 213th, id39%, 6e-30
Petroselinum crispum (Parsley)(パセリ)
Caffeoyl-CoA O-methyltransferase(EC 2.1.1.104)

caffeoyl-CoA-specific 3-O-methyltransferaseの活性測定あり。

close this sectionSequence

selected fasta
>putative O-methyltransferase [O-methyltransferase]
MGEQVVVTPALLDYLREVSQPEPAALAELRAETALLPGGGVLPVPPGEGAFLAMLVALTG
ARLVVEVGTFTGCSTLCLASALPDGGRVITCELSPKWPAIARPHWERAGVADRIEVLVGD
AAGSLAGLTGELAGRVDLVFVDADKAGYPRYYELAVELVRPGGLVVVDNTLFSGRVADPA
AVDPDTEGVRELNRRIAADDRVQAVLLAHADGMTLARRTR
selected fasta
>putative O-methyltransferase [O-methyltransferase]
GTGGGTGAGCAGGTCGTCGTCACGCCCGCGCTGCTCGACTACCTGCGCGAGGTCTCCCAG
CCGGAACCGGCGGCGCTCGCCGAGCTGCGCGCCGAGACCGCGCTGCTGCCCGGCGGCGGC
GTCCTCCCCGTCCCACCGGGGGAAGGCGCTTTCCTCGCCATGCTGGTCGCGCTCACCGGG
GCCCGGCTGGTCGTGGAGGTCGGCACCTTCACCGGGTGCAGCACGCTGTGCCTGGCCTCC
GCCCTCCCCGACGGCGGCCGGGTGATCACCTGCGAGCTGAGCCCCAAGTGGCCCGCGATC
GCCCGGCCGCACTGGGAGCGCGCGGGCGTCGCGGACCGGATCGAGGTGCTCGTCGGCGAC
GCCGCGGGCAGCCTCGCCGGGCTGACCGGCGAGCTCGCGGGCCGGGTCGACCTGGTGTTC
GTGGACGCGGACAAGGCCGGGTACCCCCGCTACTACGAGCTGGCCGTCGAGCTGGTGCGC
CCCGGCGGGCTGGTCGTCGTGGACAACACCCTGTTCTCCGGCCGGGTCGCCGACCCCGCC
GCGGTGGACCCCGACACCGAGGGCGTCCGGGAGCTCAACCGCAGGATCGCCGCCGACGAC
CGGGTCCAGGCCGTGCTGCTCGCGCACGCCGACGGCATGACCCTGGCCCGTCGCACGCGC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002935 O-methyltransferase, family 3 (Family)
[17-218] 2.6e-68 PF01596
PF01596   Methyltransf_3
SignalP No significant hit
TMHMM No significant hit
Ansam_00180 Ansam_00180   Ansam_00200 Ansam_00200
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