Ansam_00200 : CDS information

Location

Organism	Actinosynnema pretiosum subsp. auranticum
Strain	ATCC 31565
Entry name	Ansamitocin
Contig	AF453501
Start / Stop / Direction	35,192 / 37,933 / + [in whole cluster] 47,555 / 44,814 / - [in contig]
Location	35192..37933 [in whole cluster] complement(44814..47555) [in contig]
Type	CDS
Length	2,742 bp (913 aa)

Annotation

Category	4.1 transcriptional regulator
Product	transcriptional activator
Product (GenBank)	transcriptional activator
Gene
Gene (GenBank)	asm18
EC number
Keyword	SARP family
Note
Note (GenBank)
Reference	ACC Q8KUG6 PmId [12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002) [26572523] Constitutive overexpression of asm18 increases the production and diversity of maytansinoids in Actinosynnema pretiosum. (Appl Microbiol Biotechnol. , 2016) comment [PMID: 12060743](2002) Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。 <cluster I> Asm18(913aa) : Transcriptional activator(Function of homologue) 本文にコメントなし。 --- [PMID: 26572523](2016) abstract SARP family regulator Asm18の機能についての調査。 asm18遺伝子の過剰発現は、asm43, asmA, asm21, asm8の転写レベルを増加させる。

Sequence

>transcriptional activator [transcriptional activator]
MRFQLLGPLEVVDRSRPVALGGTKQRAALGYLLLHPNSVVPTSSLLKALWPQDMPPTGRK
MLQNAVAALRGVLTAAGDAPGAPALLTHTPGYLLRVDPEDVDLSRFRRLVHAGRADLAAG
SWQRAARTLRDAIGLWRGPLLADLVEQGVDWPELAAVRNTRLAALEDCVEAEFASGRYGE
MIYELERWVEAEPLRERLCGQLMRALYHCGRQADALGVYRRTRARLVDELGLDPGRELRE
LERAILNQEVPHRLPARREAAPAARSGATEGGGPVAAPPVAAPPAGIVPQRQAPPAPAPA
PVALAPLPPASAPTAELKHVSVLMVLTRFEHDVAAAPPEEVDDALSDVATAVREEVERAG
GVVTAKAASLIVALFGSTRSGEDDATRAVRAALAVRDRLAHGPRGAGPAALGVTAAVATG
HALVRRHPGRAPEVGGGVLDRCMRLLAHVSPGEVRVCEETRGASEGAIGYDGPHDPVRGS
WATGARQGAGGPPATPPLVDRDRELALLRGLLEQVGRRSRTHLVTVLGEPGIGKSRLVDE
FQRVAETSATVVVRCPRLGGGGDLAPLADALAPRMGIGPADPVGLVAAKLAAAARGSGGA
GERADRVLASLRVLFGLDVPDVLPDGSFAAWRHVLEDLGGADPLVLVLEDLHWAGEALLD
FVDHLTAEATSSPLLVVVTARPELFDRRPAWGGGKAEASTITLDPLADQDVERLLTALCV
RRGPATDEASAAYRAVIAHIGGNPLFAVEYARVLADAGPSSGGGGQSPPLPPLVRRVVAA
RIDSLPADAKAVLLDAAVLGEDLCEEGVRALGGGAVPDVARALEHLERREVLRRDRDAER
ATAYAFRSPLVRDVAYLTIPQHARAEKHRRAADWLERVPGHDALRLVHHGLRSGGCRPTE
PSPVLPQPRQHAR

>transcriptional activator [transcriptional activator]
ATGCGCTTTCAGCTCTTGGGTCCGCTCGAAGTCGTCGACCGGTCCCGACCGGTGGCGCTC
GGCGGCACCAAGCAGCGCGCCGCGCTCGGCTACCTGCTGCTGCACCCGAACAGCGTGGTG
CCCACCAGCAGCCTGCTCAAGGCGCTGTGGCCGCAGGACATGCCGCCCACCGGGCGCAAG
ATGCTCCAGAACGCGGTCGCCGCCCTGCGCGGCGTGCTGACCGCCGCCGGTGACGCGCCC
GGCGCGCCCGCCCTGCTCACCCACACCCCCGGCTACCTGCTGCGCGTCGACCCGGAGGAC
GTGGACCTGAGCCGCTTCCGCAGGCTCGTCCACGCCGGGCGCGCCGACCTCGCGGCGGGC
TCCTGGCAGCGCGCCGCCCGCACCCTGCGCGACGCGATCGGCCTGTGGCGCGGGCCGCTG
CTGGCCGACCTGGTCGAGCAGGGCGTCGACTGGCCCGAGCTGGCGGCCGTGCGCAACACC
CGGCTGGCCGCGCTGGAGGACTGCGTCGAGGCGGAGTTCGCGTCCGGCCGCTACGGCGAG
ATGATCTACGAGCTGGAGCGGTGGGTCGAGGCCGAGCCGCTGCGCGAGCGGCTGTGCGGC
CAGCTCATGCGGGCGCTCTACCACTGCGGCAGGCAGGCCGACGCGCTCGGCGTGTACCGG
CGCACCAGGGCCAGGCTGGTGGACGAGCTGGGGCTCGACCCCGGCCGCGAGCTGCGCGAG
CTGGAGCGGGCGATCCTCAACCAGGAGGTGCCGCACCGGCTCCCGGCCCGGCGCGAGGCG
GCGCCCGCCGCGCGGTCCGGGGCGACCGAGGGCGGTGGCCCGGTCGCGGCGCCCCCGGTC
GCGGCGCCCCCGGCCGGGATCGTCCCGCAGCGGCAGGCGCCCCCGGCGCCCGCGCCTGCC
CCCGTCGCGCTCGCGCCCCTCCCCCCGGCGTCCGCGCCGACCGCCGAGCTCAAGCACGTC
AGCGTCCTCATGGTCCTCACCCGGTTCGAGCACGACGTGGCCGCCGCCCCGCCCGAGGAG
GTGGACGACGCGCTGAGCGACGTCGCCACCGCGGTGCGCGAGGAGGTCGAGCGGGCGGGC
GGCGTCGTCACCGCCAAGGCCGCGTCGCTGATCGTCGCCCTGTTCGGCTCCACCCGCAGC
GGCGAGGACGACGCCACCAGGGCGGTGCGCGCCGCGCTCGCCGTGCGCGACCGGCTCGCC
CACGGGCCGCGCGGCGCCGGGCCCGCCGCGCTGGGCGTCACCGCCGCCGTCGCCACCGGG
CACGCCCTGGTGCGGCGCCACCCAGGGCGCGCGCCCGAGGTCGGCGGCGGCGTGCTCGAC
CGCTGCATGAGGCTGCTCGCGCACGTCTCGCCCGGCGAGGTCAGGGTGTGCGAGGAGACC
AGGGGCGCCAGCGAGGGCGCCATCGGCTACGACGGCCCCCACGACCCGGTGCGCGGGTCC
TGGGCCACCGGGGCCAGGCAGGGCGCGGGCGGGCCCCCGGCCACGCCGCCGCTGGTGGAC
CGGGACCGCGAGCTGGCCCTGCTGCGCGGCCTGCTGGAGCAGGTCGGCCGCAGGTCCCGG
ACGCACCTGGTGACCGTGCTCGGCGAGCCCGGCATCGGCAAGAGCAGGCTCGTGGACGAG
TTCCAGCGCGTGGCCGAGACCTCGGCGACCGTTGTGGTCCGCTGCCCCCGCCTCGGCGGA
GGGGGCGACCTCGCCCCGCTCGCCGACGCGCTCGCCCCGCGCATGGGCATCGGCCCGGCC
GACCCGGTCGGGCTGGTCGCCGCCAAGCTCGCCGCCGCCGCGCGCGGCAGCGGTGGCGCG
GGCGAGCGCGCGGACCGGGTGCTGGCCAGCCTGCGCGTCCTGTTCGGGCTCGACGTGCCC
GACGTGCTCCCGGACGGCTCGTTCGCGGCGTGGCGGCACGTGCTGGAGGACCTCGGCGGC
GCGGACCCGCTCGTCCTGGTCCTGGAGGACCTGCACTGGGCGGGCGAGGCGCTGCTGGAC
TTCGTGGACCACCTCACCGCCGAGGCCACGTCGTCCCCGCTGCTGGTCGTGGTCACCGCG
CGCCCCGAGCTGTTCGACCGGCGCCCCGCCTGGGGCGGCGGCAAGGCCGAGGCCAGCACG
ATCACCCTCGACCCGCTCGCCGACCAGGACGTGGAGCGGCTGCTCACCGCCCTGTGCGTC
CGGCGCGGCCCCGCCACCGACGAGGCGAGCGCCGCCTACCGCGCGGTGATCGCCCACATC
GGCGGGAACCCGCTGTTCGCCGTCGAGTACGCCAGGGTGCTCGCCGACGCGGGCCCGTCG
TCCGGGGGCGGCGGGCAGTCGCCGCCGCTGCCGCCGCTGGTGCGCCGGGTCGTGGCGGCC
CGCATCGACAGCCTGCCCGCCGACGCCAAGGCGGTGCTGCTCGACGCGGCCGTCCTCGGC
GAGGACCTGTGCGAGGAGGGGGTGCGCGCGCTCGGCGGCGGCGCCGTGCCCGACGTGGCG
CGCGCGCTGGAGCACCTGGAGCGGCGCGAGGTCCTGCGCCGGGACCGGGACGCCGAGCGC
GCCACCGCCTACGCGTTCCGCAGCCCGCTCGTGCGCGACGTCGCCTACCTGACCATCCCC
CAGCACGCCCGCGCCGAGAAGCACCGGCGAGCGGCCGACTGGTTGGAGCGCGTGCCCGGT
CACGACGCGCTGCGGCTGGTCCACCACGGGCTCAGGTCGGGCGGGTGCCGCCCGACCGAG
CCCTCGCCGGTGCTCCCGCAACCCCGGCAGCACGCGCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001054 Adenylyl cyclase class-3/4/guanylyl cyclase (Domain) SSF55073 A/G_cyclase IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Trans_reg_C PF00486 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Ansam_00190 Ansam_00210