Ansam_00490 Ansam_00490   Ansam_00510 Ansam_00510

Ansam_00500 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction90,958 / 91,764 / + [in whole cluster]
8,212 / 9,018 / + [in contig]
Location90958..91764 [in whole cluster]
8212..9018 [in contig]
TypeCDS
Length807 bp (268 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)unknown
Geneasm46
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF9
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

cluster II を削除するとansamitocinsの産生がなくなるが、AHBAを補充すると産生は回復するので、cluster II はAHBA形成のみに必要な遺伝子を含む。

<cluster II>
Asm46(266aa) : Unknown

asm43-47は明らかにひとつのオペロンで編成される。
Asm46は知られた遺伝子に配列類似を示さない。

close this sectionSequence

selected fasta
>hypothetical protein [unknown]
MVVDMTRTGSAQAPLGGPPAAGAPSWSGWSLAGGGDARPVAEVLPPVVRELRARDRGAHW
SFLRGTGVALWLGTTEAARGPVLAVLRAEAAARELRVDPAGEGLPGGPVEPLVEVATASS
ELALDVAAAVGPAWGEDPGGSGAQATVRRHLAELVALAPEPARPAFLFHCWQSWGRPLSP
AARTEAAVAAQGPSPGGRVVGSAGAWRRYLEQIDRAQLRTAADRRRPALPVLFEQATLTH
NRLGVGAAVAARAALALRTALVTGAACG
selected fasta
>hypothetical protein [unknown]
GTGGTGGTGGACATGACGCGGACCGGTTCCGCGCAAGCGCCCCTCGGTGGCCCCCCGGCG
GCGGGCGCCCCGTCCTGGTCCGGCTGGTCGCTGGCCGGTGGTGGCGACGCCCGCCCGGTG
GCCGAGGTGCTGCCCCCCGTCGTGCGGGAGCTGCGCGCGCGGGACCGGGGCGCGCACTGG
TCGTTCCTGCGCGGGACGGGGGTGGCGCTGTGGCTGGGCACGACCGAGGCCGCGCGGGGC
CCGGTGCTCGCCGTGCTGCGCGCCGAGGCGGCGGCCCGCGAGCTGCGCGTCGACCCGGCG
GGCGAGGGCTTGCCGGGCGGTCCGGTCGAGCCGCTGGTCGAGGTCGCGACGGCCTCCAGC
GAACTGGCGCTGGACGTCGCGGCGGCGGTGGGCCCGGCGTGGGGCGAGGACCCCGGCGGG
TCGGGCGCGCAGGCCACGGTGCGGCGCCACCTGGCCGAACTGGTCGCGCTGGCCCCGGAA
CCGGCCAGGCCCGCGTTCCTGTTCCACTGCTGGCAGAGCTGGGGCAGACCGCTCAGCCCG
GCCGCGCGCACCGAGGCCGCCGTCGCCGCGCAGGGCCCGTCGCCCGGCGGGCGCGTGGTC
GGCTCGGCCGGCGCGTGGCGGCGCTACCTGGAACAGATCGATCGCGCGCAGCTGCGCACC
GCGGCGGACCGCCGCCGCCCGGCCCTGCCGGTGCTGTTCGAGCAGGCGACCCTGACCCAC
AACCGCCTCGGCGTCGGCGCGGCCGTGGCGGCGCGGGCGGCGCTCGCCCTGCGCACCGCG
CTCGTGACGGGCGCCGCCTGCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro No significant hit
SignalP No significant hit
TMHMM No significant hit
Ansam_00490 Ansam_00490   Ansam_00510 Ansam_00510
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