Azino_00100 Azino_00100   Azino_00120 Azino_00120

Azino_00110 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction22,185 / 20,878 / - [in whole cluster]
22,185 / 20,878 / - [in contig]
Locationcomplement(20878..22185) [in whole cluster]
complement(20878..22185) [in contig]
TypeCDS
Length1,308 bp (435 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative sulfate adenylyltransferase subunit 1
putative ATP sulfurylase large subunit
Product (GenBank)Azi10
GeneaziH2
cysN
Gene (GenBank)azi10
EC number2.7.7.4
Keyword
Note
Note (GenBank)
  • sulfate adenylyltransferase subunit 1
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
comment
Azinomycin B生合成遺伝子クラスターの報告。

aziH2: Sulfate adenylyltransferase subunit 1

AziF, AziG, AziH1, AziH2, AziH3 の機能は、azinomycin B生合成経路に割り当てることができなかった。
AziH1, AziH2, AziH3 のsubsetは、sulfur metabolismの最初の2段階を行うとされる酵素（E.coliのCysD, CysN, CysCなど）に高い類似性を示す。
Related Reference
ACC
Q10600
PmId
comment
Blast id58%, C末余る
Mycobacterium tuberculosis_cysNC
Bifunctional enzyme cysN/cysC

E.coliで発現、精製、活性を見ている。cysDとcysNCはオペロン。
Sulfur非存在下でcysDNC operonは発現が誘導され、主な最終産物であるCysteineの添加により発現が抑制される。

CysDNCは、sulfate activation pathwayを構成するATP sulfurylase, GTPase, adenosine 5'-phosphosulfate kinase活性を示す多機能酵素複合体を形成する。
ACC
P23845
PmId
[2828368] ( , )
comment
Blast id48%
Escherichia coli_cysN
Sulfate adenylyltransferase subunit 1(EC 2.7.7.4)

大量発現後、精製。
ATP sulfurylaseはCysN(62kDa)とCysD(27kDa)の2つのsubunitから構成されている。
cysN破壊株はsulfate activationが妨げられる。
またcysNとこの下流の遺伝子cysCはオペロンを形成している。

close this sectionSequence

selected fasta
>putative sulfate adenylyltransferase subunit 1 [Azi10]
MSGSTGRWAGPSATALLRFATAGSVDDGKSTLVGRLLYDSKSVLTDQWEAVERASRHRGQ
DAPDLALLTDGLRAEREQGITIDVAYRYFATARRRFILADTPGHVQYTRNMVTGASTAEL
ALVLVDARHGVVEQTRRHATIAALLRVPHLVLAVNKMDLAGYEARVFARIAEEFTAYATE
LGVPEVTAIPISALVGDNVVEPSANMDWYGGPTVLEHLETVPVSHDLTGCHARLPVQYVI
RPRTAEHPDYRGYAGQIAAGAFRVGQEVTVLPSGATSRISGIDVLGEAVGEARAPQSVTL
LLEDDIDVARGDLLVPSGDGPVARRDVEATVCHVADAPLAVGQRVLLKHTTRTVRAIVAE
IRSRLVLDGIVAHPEPGQLVANDIGRVRIRTAEPLAVDAYADSRRTGSFLLIDPADGTTL
AAGMAGPAFGAAVTG
selected fasta
>putative sulfate adenylyltransferase subunit 1 [Azi10]
ATGAGTGGATCCACCGGACGGTGGGCCGGTCCGTCGGCCACCGCACTGCTGCGTTTCGCG
ACGGCCGGGTCGGTCGACGACGGCAAATCCACCCTCGTCGGCCGTCTTCTGTACGACTCC
AAGTCGGTCCTCACCGACCAGTGGGAGGCCGTCGAGCGCGCCTCCCGCCACCGCGGCCAG
GACGCCCCCGACCTGGCGCTGCTCACCGACGGCCTGCGCGCGGAGCGGGAGCAGGGCATC
ACCATCGACGTCGCCTACCGCTACTTCGCCACCGCCCGGCGCCGGTTCATCCTGGCGGAC
ACCCCCGGGCACGTGCAGTACACGCGGAACATGGTGACGGGCGCGTCCACGGCCGAGCTG
GCCCTCGTCCTCGTCGACGCCCGCCACGGCGTGGTCGAGCAGACCCGCAGGCACGCCACC
ATCGCCGCCCTGCTGCGCGTCCCGCACCTCGTCCTCGCCGTCAACAAGATGGACCTGGCG
GGCTACGAGGCGCGGGTGTTCGCCCGGATCGCCGAGGAGTTCACCGCGTACGCGACGGAG
CTGGGCGTCCCGGAGGTCACCGCGATCCCGATCTCCGCGCTCGTCGGCGACAACGTCGTG
GAACCGTCGGCGAACATGGACTGGTACGGCGGTCCCACCGTCCTGGAGCACCTGGAGACG
GTCCCGGTCAGCCACGACCTGACCGGCTGCCACGCCCGCCTCCCCGTCCAGTACGTGATC
AGGCCGCGGACCGCCGAGCACCCGGACTACCGCGGCTACGCGGGTCAGATCGCCGCCGGT
GCGTTCCGCGTCGGCCAGGAGGTCACGGTCCTGCCCTCCGGCGCCACCTCGCGCATCAGC
GGTATCGACGTGCTGGGCGAAGCGGTCGGCGAGGCGCGGGCGCCGCAGTCGGTGACGCTC
CTGCTGGAGGACGACATCGACGTCGCGCGCGGTGACCTCCTGGTGCCGTCAGGGGACGGC
CCGGTCGCCCGCCGGGACGTCGAGGCGACGGTCTGCCACGTCGCGGACGCGCCGCTCGCG
GTCGGGCAGCGGGTGCTCCTCAAACACACCACCCGTACCGTCAGAGCCATCGTCGCGGAG
ATCCGGTCCCGGCTCGTCCTGGACGGGATCGTCGCGCACCCGGAGCCCGGGCAGCTGGTC
GCCAATGACATCGGCCGGGTCCGGATCCGTACCGCGGAGCCGCTCGCCGTGGACGCCTAC
GCCGACTCGCGGCGCACCGGCTCCTTCCTGCTGATCGACCCGGCCGACGGGACGACGCTC
GCCGCGGGCATGGCGGGTCCCGCGTTCGGCGCGGCCGTCACCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000795 Protein synthesis factor, GTP-binding (Domain)
[18-31] 3.7999986264479e-12 PR00315 [77-85] 3.7999986264479e-12 PR00315 [97-107] 3.7999986264479e-12 PR00315 [113-124] 3.7999986264479e-12 PR00315 [150-159] 3.7999986264479e-12 PR00315
PR00315   ELONGATNFCT
[17-200] 4.9e-41 PF00009
PF00009   GTP_EFTU
[70-85] PS00301
PS00301   EFACTOR_GTP
IPR009000 Translation elongation/initiation factor/Ribosomal, beta-barrel (Domain)
[226-322] 5.59999989417023e-18 SSF50447
SSF50447   Translat_factor
IPR009001 Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal (Domain)
[322-427] 1.50000306971104e-25 SSF50465
SSF50465   Elong_init_C
IPR011779 Sulphate adenylyltransferase, large subunit (Family)
[17-424] TIGR02034
TIGR02034   CysN
SignalP
[1-22] 0.919 Signal
Eukaryota   
TMHMM No significant hit
Azino_00100 Azino_00100   Azino_00120 Azino_00120
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