Azino_00110 Azino_00110   Azino_00130 Azino_00130

Azino_00120 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction23,021 / 22,185 / - [in whole cluster]
23,021 / 22,185 / - [in contig]
Locationcomplement(22185..23021) [in whole cluster]
complement(22185..23021) [in contig]
TypeCDS
Length837 bp (278 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative sulfate adenylyltransferase subunit 2
putative ATP sulfurylase small subunit
Product (GenBank)Azi11
GeneaziH1
cysD
Gene (GenBank)azi11
EC number2.7.7.4
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • sulfate adenylyltransferase subunit 2
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
comment
Azinomycin B生合成遺伝子クラスターの報告。

aziH1: Sulfate adenylyltransferase subunit 2

AziF, AziG, AziH1, AziH2, AziH3 の機能は、azinomycin B生合成経路に割り当てることができなかった。
AziH1, AziH2, AziH3 のsubsetは、sulfur metabolismの最初の2段階を行うとされる酵素（E.coliのCysD, CysN, CysCなど）に高い類似性を示す。
Related Reference
ACC
P65670
PmId
comment
Blast id65%
Mycobacterium tuberculosis_cysD
Sulfate adenylyltransferase subunit 2

E.coliで発現、精製、活性を見ている。cysDとcysNCはオペロン。
Sulfur非存在下でcysDNC operonは発現が誘導され、主な最終産物であるCysteineの添加により発現が抑制される。

CysDNCは、sulfate activation pathwayを構成するATP sulfurylase, GTPase, adenosine 5'-phosphosulfate kinase活性を示す多機能酵素複合体を形成する。
ACC
P21156
PmId
[2828368] ( , )
comment
Blast id47%
Escherichia coli_cysD
Sulfate adenylyltransferase subunit 2(EC 2.7.7.4)

cysDのクローニング、マッピング、遺伝子産物同定、特徴づけあり。
ATP sulfurylaseはCysN(62kDa)とCysD(27kDa)という2つのsubunitから構成されている。

close this sectionSequence

selected fasta
>putative sulfate adenylyltransferase subunit 2 [Azi11]
MAGEFEKPVILFSGGKDSIVMLHLALKAFAPAPVPFSLLHVDTGHNFPEVLAYRDRAVAR
HGLRLHVASVQDYIDRGVLKERPDGTRNPLQTVPLTEKIHSERFDAVLGGGRRDEEKARA
KERVFSLRDEFSQWDPRRQRPELWQLYNGRHGPGEHVRVFPLSNWTELDVWQYIAREDIE
LPGIYYAHERPVFRRNGMWLTAGEWGGPKDGERVETRLVRYRTVGDMSCTGAVDSDAVTL
DQVVAEIATSRLTERGATRADDRLSEAAMEDRKREGYF
selected fasta
>putative sulfate adenylyltransferase subunit 2 [Azi11]
GTGGCGGGCGAGTTCGAGAAGCCGGTGATCCTGTTCTCCGGCGGCAAGGACTCGATCGTC
ATGCTGCACCTGGCGCTCAAGGCGTTCGCGCCCGCGCCGGTCCCCTTCTCGCTGCTGCAC
GTCGACACCGGGCACAACTTCCCCGAGGTCCTCGCCTACCGCGACCGCGCGGTGGCCCGC
CACGGACTGCGGCTGCACGTCGCCTCCGTGCAGGACTACATCGACCGCGGGGTGCTCAAG
GAGCGCCCGGACGGCACCCGCAACCCGCTGCAGACCGTGCCGCTCACGGAGAAGATCCAC
AGCGAGCGGTTCGACGCGGTCCTCGGCGGCGGGCGCCGCGACGAGGAGAAGGCCCGCGCC
AAGGAGCGCGTGTTCAGTCTCCGCGACGAGTTCTCGCAGTGGGACCCGCGCCGCCAGCGC
CCGGAACTGTGGCAGCTGTACAACGGGCGGCACGGCCCCGGCGAGCACGTCCGCGTCTTC
CCGCTGTCCAACTGGACCGAGCTGGACGTGTGGCAGTACATCGCCCGCGAGGACATCGAA
CTCCCCGGGATCTACTACGCCCACGAGCGCCCGGTGTTCCGCCGCAACGGCATGTGGCTG
ACCGCCGGCGAGTGGGGCGGCCCCAAGGACGGCGAACGCGTCGAGACCCGGCTGGTCCGC
TACCGCACGGTCGGCGACATGTCCTGCACCGGCGCCGTCGACTCCGACGCGGTGACGCTG
GACCAGGTCGTCGCCGAGATCGCCACGTCCCGGCTCACCGAGCGGGGCGCCACCCGGGCC
GACGACCGGCTGTCCGAGGCCGCGATGGAGGACCGCAAGCGTGAGGGGTACTTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002500 Phosphoadenosine phosphosulphate reductase (Domain)
[8-232] 3.5e-47 PF01507
PF01507   PAPS_reduct
IPR011784 Sulphate adenylyltransferase, small subunit (Family)
[2-278] 5.9e-129 TIGR02039
TIGR02039   CysD: sulfate adenylyltransferase, small subunit
IPR014729 Rossmann-like alpha/beta/alpha sandwich fold (Domain)
[218-227] 1.4e-45 G3DSA:3.40.50.620
G3DSA:3.40.50.620   no description
SignalP No significant hit
TMHMM No significant hit
Azino_00110 Azino_00110   Azino_00130 Azino_00130
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