Azino_00160 Azino_00160   Azino_00180 Azino_00180

Azino_00170 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction26,018 / 25,755 / - [in whole cluster]
26,018 / 25,755 / - [in contig]
Locationcomplement(25755..26018) [in whole cluster]
complement(25755..26018) [in contig]
TypeCDS
Length264 bp (87 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative acyl carrier protein
Product (GenBank)Azi16
GeneaziC11
Gene (GenBank)azi16
EC number
Keyword
Note
Note (GenBank)
  • ACP/PCP
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
comment
Azinomycin B生合成遺伝子クラスターの報告。

aziC11: PCP/ACP

AziC2-C11は、3つめのbuilding blockであるaziridino[1,2a]pyrrolidinyl amino acidの生合成に関与すると想定される。

AziC11は多様な分離型PCPsに高い配列相同性を示すことから、aziridino[1,2a]pyrrolidinyl amino acidの形成に対する酵素反応が、遊離アミノ酸で起こるか、それともacyl-PCPで起こるか、という疑問が生じる。

実験的な確認はされておらず、Figure 3Cのaziridino[1,2a]pyrrolidinyl amino acid生合成提唱経路にAziC11は出てこない。
Family/Domain
FD
IPR009081
comment
[IPR009081] Acyl carrier protein-like (Domain)

close this sectionSequence

selected fasta
>putative acyl carrier protein [Azi16]
MASPGLVHDEDRIRYVQSVFRRVLRLEQDAAPDDDFFVLGGTSLAAMEVIDLIERERGLR
IPVRNFYRSTVVRALALELDPSRREAP
selected fasta
>putative acyl carrier protein [Azi16]
ATGGCGAGCCCTGGGCTGGTCCACGACGAAGACCGCATCCGTTACGTGCAGAGCGTGTTC
CGACGCGTACTCCGTCTGGAACAGGACGCGGCCCCCGACGACGACTTCTTCGTGCTCGGC
GGAACCTCGCTGGCCGCCATGGAAGTGATCGACCTGATCGAGCGGGAACGGGGGCTGCGC
ATCCCCGTACGGAACTTCTACCGCTCCACCGTGGTGCGCGCGCTGGCCCTCGAGCTCGAC
CCGTCCCGCAGGGAGGCCCCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[5-81] 7.30000590915208e-12 SSF47336
SSF47336   ACP_like
[13-81] 6.90000000000001e-15 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[12-80] PS50075
PS50075   ACP_DOMAIN
[15-77] 2e-12 PF00550
PF00550   PP-binding
SignalP No significant hit
TMHMM No significant hit
Azino_00160 Azino_00160   Azino_00180 Azino_00180
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