Azino_00180 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	27,043 / 26,021 / - [in whole cluster] 27,043 / 26,021 / - [in contig]
Location	complement(26021..27043) [in whole cluster] complement(26021..27043) [in contig]
Type	CDS
Length	1,023 bp (340 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative transketolase(fragment)
Product (GenBank)	Azi17
Gene	aziC6
Gene (GenBank)	azi17
EC number
Keyword	aziridino[1,2a]pyrrolidinyl amino acid
Note
Note (GenBank)	transketolase C-terminal subunit
Reference	ACC B4XYB0 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) comment Azinomycin B生合成遺伝子クラスターの報告。 aziC6: Transketolase C-terminal subunit AziC2-C11は、3つめのbuilding blockであるaziridino[1,2a]pyrrolidinyl amino acidの生合成に関与すると想定される。 AziC5, AziC6はそれぞれtransketolasesのN末, C末subunitに相同性あり。 C5位での2C unit伸長のためのcofactorとしてthiamine pyrophosphate(TPP)とタンパク複合体を構成すると推測している。
Related Reference	ACC P33570 PmId [8396116] ( , ) comment Escherichia coli (strain K12)_tktB Transketolase 2(EC 2.2.1.1) Azino_00190 35%, 2e-24 <-上流隣接ORF, TktBのN末部分にhit Azino_00180 26%, 0.027 <-this ORF, TktBのC末部分にhit --- [PMID:8396116](1993) tktをtktAに改名している。 tktA: 主要酵素で、transketolase activityの70-90% tktB: 活性はとても低い。transaldolaseをコードするtalAとのオペロン内にあるかもしれない。
Related Reference	ACC O06811 PmId [22645655] ( , ) comment Mycobacterium tuberculosis_tkt(Rv1449c) Transketolase (700 a.a.) 構造解析およびkinetic解析を行っている。 Azino_00190 id31%, 2e-18 <-上流隣接ORF, TktのN末部分にhit Azino_00180 id26%, 0.020 <-this ORF, TktのC末部分にhit
Family/Domain	FD IPR005475, IPR005476 comment [IPR005475] Transketolase-like, pyrimidine-binding domain (Domain) [IPR005476] Transketolase, C-terminal (Domain)

Sequence

>putative transketolase(fragment) [Azi17]
MTTARPAEQTPQPVLGPDPRAAYREALLELAAADSRVVCLDSDTGGLENTFGERFPDRYV
NVGIAEANLMTVAAGLARRGFLPYVHTMATFATMRAGEFLKLDVVGNRLPVRVVATHGGL
SAAHFGTSHFALEDLAVTRALSDLTVVVPGDARQIGPAMRQLHEVPGPAYLRLGRSATPV
PPGPAPAFRLGRARVLREGADVTLVAAGPLPLLLSLEAAAELAADGTEAQVLDLHTLHPL
DHEGLLAACRGRAGVVTVEEHRPQGGLGDAVTEVVAAELALPVRRVAVRGRPGVRVAGQR
AALEQLGVSTGAVADAARHLHAARAESPAQHTAAGPTMRG

>putative transketolase(fragment) [Azi17]
GTGACGACGGCGCGCCCCGCCGAGCAGACCCCGCAGCCGGTCCTCGGCCCGGATCCCCGG
GCCGCGTACCGCGAGGCGCTGCTGGAACTGGCCGCCGCCGACAGCCGCGTGGTCTGTCTC
GACTCCGACACCGGCGGCCTGGAGAACACCTTCGGCGAGCGCTTCCCGGACCGGTACGTC
AATGTCGGGATCGCGGAGGCGAACCTGATGACGGTGGCCGCCGGACTCGCCCGCCGCGGA
TTCCTTCCCTACGTGCACACGATGGCCACCTTCGCCACGATGCGCGCGGGCGAGTTCCTC
AAGCTGGACGTCGTGGGAAACCGGCTACCCGTACGGGTGGTGGCCACCCACGGCGGTCTC
TCCGCCGCCCACTTCGGCACCAGCCACTTCGCGCTGGAGGACCTCGCGGTGACCCGCGCC
CTGTCCGACCTGACCGTCGTCGTACCCGGTGACGCACGCCAGATCGGCCCTGCCATGCGC
CAGTTGCACGAGGTGCCCGGTCCCGCCTACCTGCGGCTGGGCCGCTCGGCCACCCCCGTG
CCGCCGGGGCCTGCCCCCGCCTTCCGGCTGGGCCGCGCCCGGGTCCTGCGCGAGGGCGCC
GACGTCACGCTGGTGGCCGCGGGACCGCTGCCCCTGCTCCTCTCGCTGGAGGCCGCGGCA
GAACTGGCTGCCGACGGCACCGAGGCGCAGGTCCTGGACCTGCACACCCTGCACCCGTTG
GACCACGAGGGCCTGTTGGCGGCGTGCCGGGGCCGCGCCGGAGTGGTGACGGTGGAGGAG
CACCGGCCGCAAGGCGGGCTGGGCGACGCGGTCACCGAGGTGGTCGCCGCCGAACTGGCT
CTCCCGGTGCGCCGCGTGGCCGTACGAGGCCGTCCCGGCGTGCGCGTCGCCGGCCAGCGG
GCCGCGCTGGAGCAGCTCGGCGTGAGCACCGGCGCGGTGGCGGACGCGGCCCGGCACCTG
CACGCAGCACGAGCCGAATCACCGGCGCAGCACACTGCCGCCGGCCCAACCATGAGGGGA
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005475 Transketolase-like, pyrimidine-binding domain (Domain) SM00861 Transket_pyr PF02779 Transket_pyr IPR005476 Transketolase, C-terminal (Domain) PF02780 Transketolase_C IPR009014 Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II (Domain) SSF52922 Transketo_C_like IPR015941 Transketolase-like, C-terminal (Domain) G3DSA:3.40.50.920 Transketo_C_like
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Azino_00170 Azino_00190