Azino_00260 Azino_00260   Azino_00280 Azino_00280

Azino_00270 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction44,054 / 44,827 / + [in whole cluster]
44,054 / 44,827 / + [in contig]
Location44054..44827 [in whole cluster]
44054..44827 [in contig]
TypeCDS
Length774 bp (257 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)Azi27
GeneaziU1
Gene (GenBank)azi27
EC number
Keyword
Note
Note (GenBank)
  • conserved hypothetical protein
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
[23039858] Essential role of an unknown gene aziU3 in the production of antitumor antibiotic azinomycin B verified by utilizing optimized genetic manipulation systems for Streptomyces sahachiroi. (FEMS Microbiol Lett. , 2012)
comment
[PMID:18635006](2008)
Azinomycin B生合成遺伝子クラスターの報告。

aziU1: Unknown protein

aziU1,U2,U3はstart and stop codonsがoverlapしていることから、翻訳共役される可能性が示唆された。
配列からの機能推定はできなかった。
これらのORFがazinomycin Bの生合成に関与するかはわかっていない。

---
[PMID:23039858](2012)
in-fame deletion and complementation experimentsの結果から、3つのunknown genes (aziU1, aziU2 and aziU3)は、azinomycin B 生合成に必須の遺伝子であることが示された。

AziU1の機能解析はされていない。

close this sectionSequence

selected fasta
>hypothetical protein [Azi27]
MPGQDSVLIEESIPGRLRADHFAQHGWAHGRLSVPTEGSDWEQFASAWDELVPDPYLGEE
RCRRNRRFGRVLAHRDGALEPLRGSDFFQSKEINRAFGGRLRVFEPLTDAVLTGPCLPRL
LRETVAVVNEAAGKQDWELGIHFIRVIADPGESSEPAPEGRHSDGHAYVAIHLIDRHQCA
GGRNQLFRNNESRAQFSVVMTEPLETLIVSDTTMEHSVSEIRPEAGTGSGWRDTMIVDFN
RVPVPEEVTGRTYGFIR
selected fasta
>hypothetical protein [Azi27]
ATGCCAGGTCAGGACTCAGTGCTGATCGAGGAATCGATACCTGGAAGGTTGCGCGCCGAT
CACTTCGCTCAGCACGGTTGGGCACACGGCCGCCTGAGCGTGCCCACCGAGGGAAGCGAC
TGGGAGCAATTCGCCTCGGCCTGGGACGAACTCGTCCCGGACCCCTACCTCGGCGAGGAG
CGGTGCCGTCGCAACCGGCGGTTCGGCAGGGTGCTCGCGCACCGGGACGGTGCGCTGGAG
CCGCTGCGCGGATCCGACTTCTTCCAGAGCAAGGAGATCAACCGCGCCTTCGGCGGCCGG
CTGCGCGTGTTCGAGCCGCTGACCGATGCCGTACTGACGGGTCCTTGCCTTCCCCGGTTG
CTGCGCGAGACCGTCGCCGTCGTCAACGAGGCGGCGGGGAAGCAGGACTGGGAACTCGGC
ATCCACTTCATCCGGGTCATCGCGGATCCTGGTGAAAGCTCGGAACCGGCACCGGAGGGC
CGTCATTCGGACGGTCACGCCTACGTGGCCATACACCTGATCGACCGGCACCAGTGTGCC
GGAGGACGTAACCAACTGTTCCGGAACAACGAGTCGCGGGCACAGTTCTCGGTCGTCATG
ACCGAGCCGCTGGAGACCCTGATCGTGTCCGACACGACGATGGAACACAGCGTCAGCGAG
ATCCGGCCCGAAGCCGGCACCGGGTCCGGCTGGCGGGACACCATGATCGTCGACTTCAAC
CGTGTGCCCGTCCCGGAGGAAGTGACAGGACGCACGTACGGATTCATCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR018724 Protein of unknown function DUF2257 (Family)
[44-239] 1.70000000000001e-54 PF10014
PF10014   BsmA
SignalP
[1-33] 0.034 Signal
Eukaryota   
[1-28] 0.055 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Azino_00260 Azino_00260   Azino_00280 Azino_00280
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