Azino_00270 Azino_00270   Azino_00290 Azino_00290

Azino_00280 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction44,838 / 45,503 / + [in whole cluster]
44,838 / 45,503 / + [in contig]
Location44838..45503 [in whole cluster]
44838..45503 [in contig]
TypeCDS
Length666 bp (221 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)Azi28
GeneaziU2
Gene (GenBank)azi28
EC number
Keyword
Note
Note (GenBank)
  • hypothetical protein
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
[23039858] Essential role of an unknown gene aziU3 in the production of antitumor antibiotic azinomycin B verified by utilizing optimized genetic manipulation systems for Streptomyces sahachiroi. (FEMS Microbiol Lett. , 2012)
comment
[PMID:18635006](2008)
Azinomycin B生合成遺伝子クラスターの報告。

aziU2: Unknown protein

aziU1,U2,U3はstart and stop codonsがoverlapしていることから、翻訳共役される可能性が示唆された。
配列からの機能推定はできなかった。
これらのORFがazinomycin Bの生合成に関与するかはわかっていない。

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[PMID:23039858](2012)
in-fame deletion and complementation experimentsの結果から、3つのunknown genes (aziU1, aziU2 and aziU3)は、azinomycin B 生合成に必須の遺伝子であることが示された。

AziU2の機能解析はされていない。

close this sectionSequence

selected fasta
>hypothetical protein [Azi28]
MTHVAETSAPTRSEPDTRVLTLPGTASAPEFRLIDIDGLLNNRATTDVRDLGSGRLNAWG
NSFPAAELPAPGSLITVAGIPFTWANAHARGDNIRCEGQVVDIPPGQYDWIYLLAASERR
SEDTIWAHYDDGHADPLRVGISDFLDGTPAFGELSAFRTSRMHYPHHVQEGLPTTMWLTR
VGMPRHGVARSLRLPRSVAMHVFALTLRTAAAVRLAEGATT
selected fasta
>hypothetical protein [Azi28]
ATGACTCACGTGGCAGAGACTTCGGCACCCACCCGAAGCGAACCGGATACGCGCGTGCTC
ACACTGCCCGGAACGGCATCGGCACCGGAGTTCCGCCTGATCGACATCGACGGCCTGCTC
AACAACCGGGCGACGACCGACGTCCGCGACCTCGGCTCCGGGCGGCTGAACGCCTGGGGA
AACTCCTTCCCGGCAGCGGAACTTCCCGCGCCCGGATCCCTGATCACGGTGGCCGGCATT
CCCTTCACCTGGGCGAACGCGCACGCGAGGGGGGACAACATCCGGTGCGAAGGACAGGTC
GTCGACATCCCGCCGGGCCAGTACGACTGGATCTATCTCCTCGCCGCCTCGGAACGGCGC
AGCGAGGACACCATCTGGGCCCACTACGACGACGGGCACGCCGATCCGCTCCGGGTCGGA
ATCTCGGACTTCCTGGACGGCACCCCCGCGTTCGGAGAGCTCAGTGCCTTCCGCACCTCC
CGGATGCACTACCCGCACCACGTCCAGGAAGGGCTGCCCACCACGATGTGGCTGACCCGC
GTGGGGATGCCGCGCCACGGGGTCGCCCGGTCCCTGCGGCTGCCGCGTTCGGTCGCGATG
CACGTCTTCGCGCTCACCCTGCGGACCGCGGCCGCCGTACGACTCGCGGAAGGGGCGACG
ACATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro No significant hit
SignalP
[1-28] 0.255 Signal
Bacteria, Gram-positive   
[1-28] 0.274 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Azino_00270 Azino_00270   Azino_00290 Azino_00290
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