Azino_00340 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	50,308 / 51,456 / + [in whole cluster] 50,308 / 51,456 / + [in contig]
Location	50308..51456 [in whole cluster] 50308..51456 [in contig]
Type	CDS
Length	1,149 bp (382 aa)

Annotation

Category	5.1 general function
Product	putative acyl-CoA dehydrogenase
Product (GenBank)	Azi34
Gene	aziD3
Gene (GenBank)	azi34
EC number	1.3.99.-
Keyword
Note
Note (GenBank)	putative acyl-CoA dehydrogenase
Reference	ACC B4XYC6 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) comment Azinomycin B生合成遺伝子クラスターの報告。 aziD3: Acyl-CoA dehydrogenase AziD1, AziD2, AziD3はtailoring enzymeで、post-modificationsに関与するとしている。 AziD3は、二重結合を形成するためにC20-21で位置特異的にdehydrogenationに関与する可能性があるとしている。この二重結合はさらに酸化され、高活性のepoxide fragmentを形成しうる。配列解析のみ。
Family/Domain	FD IPR013786, IPR006091, IPR009075 comment [IPR013786] Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) 現在はIPR006092が削除され、このentryがPF02771を含む。 Molecular Function GO:0050660 flavin adenine dinucleotide binding GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors --- [IPR006091] Acyl-CoA oxidase/dehydrogenase, central domain (Domain) Molecular Function GO:0003995 acyl-CoA dehydrogenase activity --- [IPR009075] Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) 現在はIPR006090が削除され、このentryがPF00441を含む。 Molecular Function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors

Sequence

>putative acyl-CoA dehydrogenase [Azi34]
MPPQSVDFANEVEEILTAPETERLLRELATRQDGMDGDPRALYRHLGDAGILAPSWPVEY
GGRGADFTATVALLEKLVAHRIPQNLYCISVQNVGSLILAAGSEQQRRTLLPALASTELT
SCILFTEPGNGSDLAGITTTAVRDGTGWVLNGRKTYNLKSAYADIALIAVRTDPQSSQYE
GLTLFLVPLDAPGVVIRPIPSLADEQFHDIWFTDVRVDESALFGQVGEGWSLITQMFAAE
RTGLDYYARGRHWLDLVAQRVDRNGARQSDAVRTELARHEARITASKLLSCQVMQNLQDG
SPDIAESSFAKWHCSESAQRIAWWALDTLGQDLLEPGPDPGDRTLEAAFREAPGMTISGG
ASEVMLDILSSARVFTHAPRED

>putative acyl-CoA dehydrogenase [Azi34]
GTGCCACCTCAGAGCGTCGACTTCGCGAACGAGGTCGAGGAGATTCTGACGGCCCCGGAG
ACGGAACGGCTGCTGCGGGAGCTCGCCACCCGGCAGGACGGCATGGACGGCGACCCCCGT
GCCTTGTACCGGCATCTGGGCGACGCAGGGATCCTCGCTCCCTCCTGGCCGGTCGAATAC
GGCGGCAGGGGCGCCGACTTCACCGCGACCGTGGCGCTGCTGGAAAAGCTCGTCGCCCAC
CGGATACCGCAGAATCTGTACTGCATCTCGGTGCAGAACGTCGGCTCCCTGATCCTGGCC
GCGGGCTCGGAGCAGCAGCGCCGGACGCTGCTGCCCGCCCTGGCGAGCACCGAACTCACG
TCGTGCATCCTGTTCACCGAGCCCGGCAACGGATCCGACCTGGCAGGCATCACCACGACC
GCGGTGCGCGACGGCACCGGCTGGGTCCTCAACGGGCGCAAGACCTACAACCTCAAGAGC
GCCTACGCCGACATCGCGCTCATCGCGGTACGGACCGATCCGCAGAGCAGCCAGTACGAG
GGACTCACCCTCTTCCTCGTCCCGCTGGACGCACCCGGTGTCGTCATCCGCCCCATTCCC
AGCCTGGCCGACGAGCAGTTCCACGACATCTGGTTCACCGATGTGCGTGTCGACGAGTCA
GCGCTGTTCGGCCAGGTCGGCGAGGGCTGGTCGCTGATCACTCAGATGTTCGCCGCGGAG
CGCACGGGGCTGGACTACTACGCGCGGGGACGCCACTGGCTGGATCTCGTCGCGCAGCGT
GTCGACAGGAACGGTGCACGGCAGTCCGATGCCGTACGCACCGAACTGGCCCGTCACGAG
GCGCGGATCACCGCGAGCAAACTGCTGAGCTGCCAGGTCATGCAGAACCTTCAGGACGGC
AGTCCCGACATCGCCGAATCCTCCTTCGCGAAATGGCACTGCAGCGAATCGGCCCAGCGC
ATCGCCTGGTGGGCCCTGGACACCCTGGGCCAGGACCTGCTGGAACCCGGGCCCGACCCC
GGTGACCGCACTCTGGAGGCAGCCTTCCGGGAGGCACCGGGAATGACCATCTCCGGCGGG
GCCTCGGAGGTCATGCTGGACATTCTGAGCAGCGCCCGGGTATTCACCCACGCCCCGAGG
GAGGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) PF02770 Acyl-CoA_dh_M G3DSA:2.40.110.10 Acyl_CoA_DH/ox_M IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) G3DSA:1.20.140.10 AcylCoA_DH_1/2_C SSF47203 AcylCoADH_C_like IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 AcylCoA_dehyd_NM IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 AcylCoA_DH/ox_N
SignalP	No significant hit
TMHMM	No significant hit

Azino_00330 Azino_00350