Azino_00350 : CDS information

Location

Organism	Streptomyces sahachiroi
Strain	NRRL 2485 (=NBRC 13928)
Entry name	Azinomycin B
Contig	EU240558
Start / Stop / Direction	51,462 / 52,463 / + [in whole cluster] 51,462 / 52,463 / + [in contig]
Location	51462..52463 [in whole cluster] 51462..52463 [in contig]
Type	CDS
Length	1,002 bp (333 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	Azi35
Gene	aziD2
Gene (GenBank)	azi35
EC number
Keyword
Note
Note (GenBank)	putative acyl-CoA dehydrogenase family protein
Reference	ACC B4XYC7 PmId [18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008) comment Azinomycin B生合成遺伝子クラスターの報告。 aziD2: Acyl-CoA dehydrogenase AziD1,AziD2,AziD3はtailoring enzymeで、post-modificationsに関与するとしている。配列類似性から、AziD2は2-amino-1,3-dicarbonyl基を与えるためにhydroxyl基を酸化する候補だと推定している。
Family/Domain	FD IPR009075 comment [IPR009075] Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) 現在はIPR006090が削除され、このentryがPF00441を含む。 Molecular Function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors

Sequence

>putative oxidoreductase [Azi35]
MDLRPDPFQQRLRSAVDTVLTRTGDLPAQLADIGVPSLGAPERLGGFALGLSADIVVNER
LGHGLEPLDSYRATAFALDLLDCDGAPTELLAEILADLYKGTRHAVVVGARGGGAVQVRD
GGRLWGESESLPAGDIALCIVRTAGEDGAAWRLAIPEPGTCVTEPAEHLGIPASRLRFHG
APSQSLPVTTAHWQRALAAARIRQAALLLGIADWITDVARAHVNTRTQSGKPLVERQTVA
HRLAVLIGEGEGWKLLLNKAAWEFDRGGRPDAAAMLAVAGEHAQLASRIALQLHGVRGML
AHSTAASVYRMVAVESARLGTPAALWREAGSAA

>putative oxidoreductase [Azi35]
ATGGACCTGCGACCTGATCCGTTCCAGCAGCGTCTGCGCAGCGCCGTGGACACGGTCCTC
ACGCGCACCGGCGACCTCCCCGCTCAACTGGCGGACATCGGCGTGCCCTCCCTCGGCGCA
CCGGAGCGCCTCGGCGGCTTCGCGCTCGGACTCAGCGCCGACATCGTCGTGAACGAACGC
CTCGGACACGGACTCGAACCGCTCGACAGCTATCGGGCGACCGCGTTCGCGCTCGACCTG
CTCGACTGCGACGGCGCACCCACGGAACTGCTGGCCGAGATCCTCGCTGACCTGTACAAG
GGCACCCGGCACGCGGTGGTCGTGGGGGCGCGAGGCGGCGGCGCCGTACAAGTGCGGGAC
GGAGGCCGGCTGTGGGGCGAGTCGGAATCGCTGCCTGCGGGTGACATCGCGCTGTGCATC
GTGCGAACCGCCGGCGAGGACGGCGCCGCATGGCGTCTGGCGATTCCGGAACCCGGCACC
TGCGTCACGGAGCCGGCGGAGCATCTCGGCATACCCGCCTCGCGATTGAGATTCCACGGT
GCGCCGTCCCAGTCTCTGCCGGTGACCACCGCTCACTGGCAGCGGGCCCTGGCGGCGGCG
CGGATCCGGCAGGCGGCACTGCTGCTGGGCATCGCCGACTGGATCACCGATGTGGCCCGC
GCCCATGTCAACACCCGCACGCAGTCCGGCAAACCGCTCGTGGAGCGGCAGACGGTCGCC
CACCGGCTGGCCGTCCTGATCGGCGAGGGGGAGGGCTGGAAGCTCCTGCTGAACAAGGCG
GCCTGGGAGTTCGACCGCGGCGGGCGCCCCGACGCGGCGGCGATGCTGGCGGTCGCCGGC
GAACACGCGCAGCTCGCCAGCCGCATCGCCCTCCAACTGCACGGCGTACGGGGAATGCTC
GCGCACTCGACAGCGGCATCCGTGTACCGGATGGTGGCCGTGGAGTCGGCGCGGCTGGGC
ACCCCCGCCGCGCTGTGGCGGGAAGCCGGATCCGCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) SSF47203 AcylCoADH_C_like G3DSA:1.20.140.10 AcylCoA_DH_1/2_C
SignalP	No significant hit
TMHMM	No significant hit

Azino_00340 Azino_00360