Azino_00350 Azino_00350   Azino_00370 Azino_00370

Azino_00360 : CDS information

close this sectionLocation

Organism
StrainNRRL 2485 (=NBRC 13928)
Entry nameAzinomycin B
Contig
Start / Stop / Direction52,571 / 53,686 / + [in whole cluster]
52,571 / 53,686 / + [in contig]
Location52571..53686 [in whole cluster]
52571..53686 [in contig]
TypeCDS
Length1,116 bp (371 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
ProductAzinomycin B resistance protein
DNA glycosylase/DNA binding protein
Product (GenBank)Azi36
Geneorf1
Gene (GenBank)azi36
EC number
Keyword
Note
  • bifunctional protein
Note (GenBank)
  • hypothetical protein
Reference
ACC
PmId
[18635006] Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis. (Chem Biol. , 2008)
[26400161] Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage. (Nucleic Acids Res. , 2016)
comment
[PMID: 18635006](2008)
Azinomycin B生合成遺伝子クラスターの報告。

このORFはazi gene clusterの外にある。
azi gene clusterの右側の境界は、putative transposaseをコードするorf4と、二次代謝生合成に関与するものに有意な類似性のないhypothetic genes (orf1-3)によって判断された。

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[PMID: 26400161](2016)
Orf1は、新規DNA結合タンパク質としてゲノムDNAに大量に結合し薬物(azinomycin B)攻撃によるリスクを減少させ、新規DNA glycosylaseとしてazinomycin BによるDNA損傷に対し塩基除去修復システムを始動させる、bifunctional proteinである。

close this sectionSequence

selected fasta
>Azinomycin B resistance protein [Azi36]
MKASWRQVFAWRMQRQFLEPRTQPSASDVVGRLCGVQAQVWSVAELNVALRQAAPDRESV
NREVADLSLMKTWAMRGTLHLLRPSEAGPYLSLMANTGSWLKPSWTRASGVTPRQVDELT
EEVAGILDGVVLTRDELVTRLVADKRFVSMEERLRSGWGSVLKPLAWRGVLCHGPNRGNK
ITFTLPASQFGADWGKMPEPDEAAPTVIKAYLGAYGPATIETFDRWLSLNSTSKPKLRKW
FGDMGDELTEVDVEGRKAFVLTEHAEELAATAPCTGIRLLGGFDQYLLGPGTKDEVVLAP
EHRSAVSRAAGWISPVVVKDGRVVGVWEIVDQELVVTPFPDTERLPVKAVEKEAAHVARA
SGVSRLPVRIV
selected fasta
>Azinomycin B resistance protein [Azi36]
GTGAAGGCCAGCTGGAGACAGGTATTTGCGTGGCGGATGCAGCGGCAGTTCCTGGAGCCG
CGTACCCAGCCGAGTGCGAGTGACGTCGTCGGCAGACTCTGTGGCGTGCAGGCACAGGTC
TGGAGTGTCGCCGAGCTGAACGTCGCACTTCGGCAGGCGGCACCCGACCGGGAAAGCGTC
AACCGGGAGGTCGCCGACCTCTCGTTGATGAAGACCTGGGCGATGCGCGGAACCCTGCAC
CTCCTGCGGCCGAGCGAGGCCGGGCCCTACCTGTCGCTCATGGCGAACACGGGAAGCTGG
CTCAAGCCGTCCTGGACCCGGGCCTCCGGCGTCACGCCCCGACAGGTCGACGAGCTGACC
GAAGAGGTGGCGGGAATCCTCGACGGAGTGGTGCTCACCCGGGATGAGCTCGTCACCCGG
CTCGTCGCCGACAAGCGGTTCGTGAGCATGGAGGAGCGACTGCGGTCGGGGTGGGGCTCC
GTGCTCAAACCGCTGGCCTGGCGAGGCGTCCTGTGCCACGGCCCGAACCGGGGCAACAAG
ATCACGTTCACGCTGCCGGCGAGCCAGTTCGGTGCGGACTGGGGCAAGATGCCCGAACCC
GACGAGGCGGCGCCCACGGTGATCAAGGCCTACCTGGGCGCGTACGGCCCTGCGACGATC
GAGACCTTCGACCGCTGGCTGTCGCTCAACAGCACGAGCAAGCCGAAGCTGCGCAAGTGG
TTCGGCGACATGGGCGACGAGCTCACCGAGGTCGACGTCGAGGGGCGCAAGGCCTTCGTC
CTGACGGAGCACGCGGAGGAACTCGCCGCCACCGCCCCGTGCACCGGCATCAGGCTGCTC
GGCGGCTTCGACCAGTACCTCCTCGGCCCCGGAACCAAGGACGAGGTCGTCCTCGCACCG
GAACACCGCTCCGCGGTGAGCCGCGCCGCCGGCTGGATCTCCCCCGTGGTCGTGAAGGAC
GGCAGAGTCGTCGGGGTGTGGGAGATCGTCGACCAGGAGCTGGTGGTGACCCCGTTCCCG
GACACCGAACGGCTCCCTGTGAAGGCGGTCGAGAAGGAGGCCGCGCACGTCGCCCGCGCC
AGCGGAGTGAGCCGGCTGCCGGTGCGCATCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009351 Protein of unknown function DUF1006 (Family)
[11-359] 4.10000000000004e-52 PF06224
PF06224   DUF1006
SignalP No significant hit
TMHMM No significant hit
Azino_00350 Azino_00350   Azino_00370 Azino_00370
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