Rever_00030 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	2,899 / 1,874 / - [in whole cluster] 2,899 / 1,874 / - [in contig]
Location	complement(1874..2899) [in whole cluster] complement(1874..2899) [in contig]
Type	CDS
Length	1,026 bp (341 aa)

Annotation

Category
Product
Product (GenBank)	putative amidohydrolase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf-2
Reference	ACC G1UDT3 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative amidohydrolase]
MIIDCHGHYTTAPAALGDWRDRQVRALAGDGEPPRPADLRIGDDEIRETIEANQLRQMDE
RGIDLTVFSPRASFMAHHVGDFAVSEAWARCCNDLCARVGELFPDRFAPAAMLPQSPGVD
PATCVPELVRAVEELGAVAVNLNPDPSGGHWSAPPLTDRSWYPLYEKIVELDVPVMVHVS
TSANPAFHTTGAHYLNADTTAFMQLLQGDLFADFPTLRMVIPHGGGTVPYHWGRFRGLAQ
ALGRPEPAEHVLGNVYFDTCVYHQPGIDLLLKVIPHANVLFASEMIGAVRGIDPDTGHHF
DDTRRYVEQAGLSAADLAAITEGNARAVYPRLDARLRASGR

>[putative amidohydrolase]
TTGATCATCGACTGCCACGGCCACTACACGACCGCACCCGCGGCTCTGGGCGACTGGCGC
GACCGCCAGGTCCGGGCCCTGGCCGGCGACGGCGAACCGCCGCGACCCGCCGACCTGCGG
ATCGGCGACGACGAGATCCGCGAGACCATCGAGGCCAACCAGCTCCGGCAGATGGACGAG
CGCGGCATCGACCTGACGGTCTTCTCCCCGCGCGCGTCGTTCATGGCCCACCACGTCGGC
GACTTCGCCGTGTCGGAGGCGTGGGCACGGTGCTGCAACGACCTGTGCGCCCGGGTCGGC
GAGCTGTTCCCCGACCGGTTCGCGCCGGCCGCGATGCTGCCGCAGTCGCCCGGGGTCGAT
CCGGCGACCTGCGTGCCGGAGCTGGTCCGCGCGGTCGAGGAGCTCGGCGCCGTGGCGGTC
AACCTCAACCCGGACCCCTCCGGCGGCCACTGGAGCGCCCCGCCGCTGACCGACCGCTCC
TGGTACCCGCTGTACGAGAAGATCGTCGAACTCGACGTCCCCGTGATGGTGCACGTGAGC
ACCAGTGCGAACCCCGCGTTCCACACCACCGGCGCGCACTACCTCAACGCCGACACCACC
GCCTTCATGCAGTTGCTCCAGGGCGACCTGTTCGCCGACTTCCCCACCCTGCGGATGGTC
ATCCCGCACGGCGGCGGCACCGTCCCCTATCACTGGGGGCGCTTCCGCGGCCTCGCCCAG
GCGCTCGGCAGGCCGGAGCCGGCCGAACACGTGCTGGGCAACGTCTACTTCGACACCTGC
GTCTACCACCAGCCCGGGATCGACCTGCTGTTGAAGGTGATCCCGCACGCCAACGTGCTC
TTCGCCTCGGAGATGATCGGCGCGGTGCGCGGCATCGACCCCGACACCGGGCACCACTTC
GACGACACCCGCCGCTACGTCGAGCAGGCGGGCCTGTCCGCCGCGGACCTCGCGGCGATC
ACCGAGGGCAACGCCCGCGCCGTCTACCCCCGGCTCGACGCGCGCCTGCGGGCGTCCGGC
CGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006992 Amidohydrolase 2 (Family) PF04909 Amidohydro_2
SignalP	No significant hit
TMHMM	No significant hit

Rever_00020 Rever_00040