Rever_00100 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	12,050 / 10,719 / - [in whole cluster] 12,050 / 10,719 / - [in contig]
Location	complement(10719..12050) [in whole cluster] complement(10719..12050) [in contig]
Type	CDS
Length	1,332 bp (443 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative crotonyl-CoA reductase
Product (GenBank)	putative CoA reductase carboxylase
Gene
Gene (GenBank)	revT
EC number	1.3.1.-
Keyword
Note
Note (GenBank)
Reference	ACC G1UDU0 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment 配列解析によりrevTのfunctionをCoA reductase/carboxylaseとしている。また、revR、revS、revTで2-alkylmalonyl-CoA biosynthesisとしている。実験情報は無し。
Related Reference	ACC Q53865 PmId [8521864] Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. (Eur J Biochem. , 1995) comment 43rd(Q53865) 49%, 1e-118 Streptomyces collinus_ccr Crotonyl CoA reductase 分子量からおそらくhomodimerで機能していると考えられる。 E.coliでcloning､発現､精製し、活性をみている。crotonyl-CoAとenoyl-CoA（C4）には高い基質特異性を見せるが、enoyl-CoA thioester, acryloyl-CoAのような短鎖(C3)には測定可能な活性はみられなかった。
	ACC Q9RNU6 PmId [10542184] Role of crotonyl coenzyme A reductase in determining the ratio of polyketides monensin A and monensin B produced by Streptomyces cinnamonensis. (J Bacteriol. , 1999) comment 55th(Q9RNU6) 50%, 1e-116 Streptomyces cinnamonensis_ccr Crotonyl-CoA reductase S. cinnamonensis_ccr、そのS. cinnamonensisで異種性発現させたS. collinus_ccr、ccr-disrupted mutantなどでCCR activity測定あり。 monensin A/monensin Bの比率は、CCR活性のレベルと、CCRの基質であるcrotonyl-CoAの両方に依存する。
	ACC B8XVS5 PmId [17548827] Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. (Proc Natl Acad Sci U S A. , 2007) [19458256] Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase. (Proc Natl Acad Sci U S A. , 2009) comment 85th(B8XVS5) 40%, 2e-69 Rhodobacter sphaeroides_ccr Crotonyl-CoA carboxylase/reductase この酵素だけでcrotonyl-CoAのreductionとreductive carboxylationが触媒されることを確かめている。 crotonyl-CoA＋NADPH＋CO2 → ethylmalonyl-CoA-＋NADP+

Sequence

>putative crotonyl-CoA reductase [putative CoA reductase carboxylase]
MEPMTEAVLRGADPDELSRIPVPEDFVAAHLRAEDADAFIGMDSASKDVRKSVRVGRVPM
PELAPDEVLVAVMASSINYNTVWSAMFEPISTFDALKRLGRGGGWDARHDQPYHVLGSDA
SGVIVRTGSGVRRWKVGDRVSVSPVVADTEDPRTHNDSLLSDMWAWGYETNFGGLAHYSV
VRSAQLMPKPAHLSWEEAACIPLCGGTSYRMLIGEHGARMKQGDIVLIWGATGGLGSFAV
QMVKNGGGVPIGVVSSERKAEIARKLGCDVVINRQEIGIGTQPEPGPATVKLAKRLGRAI
RQEVGEDPHIVFDYIGKATFGMSVIVVRRGGAVVTCGSSSGFEHNYDNRYLWMNLKRIIG
SHGMNLSEATETMRLSKLGKLSPVLSAVYPLAEAGEATRLVQTNAHTGKVGVLCMAPEEG
LGVTDPQLRARLGEQKLNPLRSV

>putative crotonyl-CoA reductase [putative CoA reductase carboxylase]
ATGGAACCCATGACCGAGGCAGTGCTCCGGGGCGCCGACCCGGACGAGTTGAGCCGAATT
CCGGTCCCCGAGGACTTCGTCGCCGCGCATCTGCGGGCCGAGGACGCCGACGCGTTCATC
GGCATGGACAGCGCCAGCAAGGACGTGCGCAAGTCGGTGCGGGTGGGCCGGGTGCCGATG
CCGGAACTGGCGCCCGACGAGGTGCTGGTGGCCGTGATGGCCAGCTCCATCAACTACAAC
ACCGTGTGGTCGGCGATGTTCGAGCCCATCTCGACCTTCGACGCGCTGAAGCGGCTGGGC
CGCGGCGGCGGTTGGGACGCGCGGCACGACCAGCCGTACCACGTGCTCGGCTCGGACGCG
TCCGGCGTCATCGTGCGGACCGGCTCCGGGGTGCGCCGCTGGAAGGTGGGCGACCGGGTC
TCCGTCTCCCCCGTGGTGGCGGACACCGAGGACCCCCGCACCCACAACGACAGCCTGCTC
TCCGACATGTGGGCGTGGGGCTACGAGACCAACTTCGGCGGCCTGGCGCACTACTCGGTG
GTCCGCTCCGCCCAGCTCATGCCCAAGCCCGCCCACCTGAGCTGGGAGGAGGCCGCCTGC
ATCCCGCTGTGCGGCGGCACCTCCTACCGGATGCTGATCGGCGAGCACGGCGCCCGGATG
AAGCAGGGCGACATCGTGCTGATCTGGGGCGCGACCGGCGGGCTCGGCTCGTTCGCCGTC
CAGATGGTCAAGAACGGCGGCGGCGTTCCGATCGGCGTGGTCAGCTCGGAGCGCAAGGCG
GAGATCGCCCGCAAGCTCGGCTGCGACGTCGTGATCAACCGCCAGGAGATCGGCATCGGC
ACGCAGCCGGAGCCCGGGCCGGCGACGGTGAAACTCGCCAAGCGGCTGGGCCGCGCGATC
CGCCAGGAGGTGGGCGAGGACCCGCACATCGTCTTCGACTACATCGGCAAGGCCACCTTC
GGCATGTCGGTCATCGTGGTCCGCCGCGGCGGCGCCGTCGTCACCTGCGGGTCGAGCTCC
GGCTTCGAGCACAACTACGACAACCGCTACCTGTGGATGAACCTCAAGCGCATCATCGGC
AGCCACGGGATGAACCTCAGCGAGGCGACCGAGACGATGCGGCTGAGCAAGCTCGGCAAG
CTCTCCCCGGTCCTGTCCGCGGTGTACCCGCTCGCCGAGGCCGGCGAGGCGACCCGGCTG
GTGCAGACGAACGCGCACACCGGCAAGGTCGGCGTGCTGTGCATGGCACCCGAGGAGGGA
CTCGGCGTCACCGACCCCCAGCTACGCGCCCGGCTGGGTGAGCAGAAGCTCAACCCGCTC
CGGTCCGTGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010085 Crotonyl-CoA reductase (Family) TIGR01751 Crot-CoA-red IPR011032 GroES-like (Domain) SSF50129 GroES_like IPR013149 Alcohol dehydrogenase, C-terminal (Domain) PF00107 ADH_zinc_N IPR013154 Alcohol dehydrogenase GroES-like (Domain) PF08240 ADH_N IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Rever_00090 Rever_00110