Rever_00270 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	96,795 / 98,486 / + [in whole cluster] 96,795 / 98,486 / + [in contig]
Location	96795..98486 [in whole cluster] 96795..98486 [in contig]
Type	CDS
Length	1,692 bp (563 aa)

Annotation

Category
Product
Product (GenBank)	putative transporter
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf2
Reference	ACC G1UDV7 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative transporter]
MRFTPERIALTLFTLAVVALIAAPLVEVVIRSLEKQTGLTGHVWSGANYQQLKASEILSS
ARNSVVIALGSMAVATVIGVGLSWLVTRTDLPLRRAFEILNLIPFFLSPLIGSIAWTYLA
DPRTGFINRLARSAGISHGAVFNVYSTLGIIVVLGLFLVPFVILLTGAAFRQMDSSLEHA
AAACGASGWRTAVRVTLPLASPAIMAAAILTFVMAIEDLSVPLVLGYGNGIQTLSTRIYD
AVQTFPPNYNFGAALGCVMMLITALCLYLQRRAMRDRGYVTVTGKGSRAEPLRLGRIRWW
AFAAELVYLLFAALLPIGILTLIAFSKRWTGTVDFGDLTLDNFRALFGKGSIVRTAFLNS
LELSALCACVVVALSVVLVHAIERARITGRRLFEVCLTIPVAVPGLVLGVGLLTLLLKTS
LFGTLWIIGVAYVVRFLPFAHRSVSAAFLSVHTELEEAARTSGASLGRYVFRVLLPLVRP
GLVSGWLLVFLTFMRELPMSALLQTTGTQTLSVALLNSRSFDSPGVTAAFTLLQAAVLLV
AAAVFWTITTRRDGASSDMAAGL

>[putative transporter]
GTGCGGTTCACCCCGGAGCGCATCGCGCTCACGCTGTTCACCCTGGCCGTGGTCGCCCTG
ATCGCGGCACCGCTGGTGGAAGTCGTCATCCGCTCGCTCGAGAAGCAGACCGGTCTGACC
GGCCACGTGTGGTCCGGTGCGAACTACCAGCAGCTCAAGGCGTCGGAGATCCTCAGCTCC
GCCCGCAACAGCGTGGTCATCGCCCTCGGGTCGATGGCGGTGGCCACCGTCATCGGCGTC
GGGCTGTCGTGGCTGGTCACCCGCACCGACCTGCCCCTGCGACGGGCGTTCGAGATCCTC
AACCTGATCCCGTTCTTCCTGTCCCCGCTGATCGGCTCGATCGCCTGGACCTACCTGGCC
GACCCGCGGACCGGGTTCATCAACCGGCTGGCCCGCAGCGCCGGGATCAGCCACGGGGCG
GTCTTCAACGTCTACTCCACGCTCGGCATCATCGTCGTGCTCGGCCTGTTCCTGGTGCCG
TTCGTCATCCTGCTCACCGGCGCGGCCTTCCGGCAGATGGACTCCTCGCTGGAGCACGCG
GCGGCCGCCTGCGGCGCGTCAGGCTGGCGGACGGCGGTCCGCGTGACGCTCCCGCTGGCC
AGCCCGGCGATCATGGCGGCCGCGATCCTGACGTTCGTCATGGCGATCGAGGACCTGTCG
GTGCCGCTGGTGCTCGGCTACGGCAACGGGATCCAGACGCTGTCCACCCGCATCTACGAC
GCGGTCCAGACGTTCCCGCCGAACTACAACTTCGGCGCCGCGCTCGGCTGCGTGATGATG
CTCATCACCGCGCTGTGCCTGTACCTCCAGCGCCGCGCCATGCGCGACCGCGGCTACGTC
ACGGTCACCGGCAAGGGCTCGCGGGCCGAACCGCTGCGGCTGGGGCGGATCCGCTGGTGG
GCCTTCGCGGCCGAACTGGTCTACCTGCTGTTCGCCGCGCTCCTGCCGATCGGCATCCTC
ACGCTGATCGCGTTCTCCAAACGCTGGACCGGGACGGTCGACTTCGGCGACCTGACCCTG
GACAACTTCCGCGCGCTGTTCGGGAAGGGCTCGATCGTCAGGACCGCGTTCCTCAACAGC
CTGGAGCTGTCCGCGCTGTGCGCCTGCGTGGTCGTCGCGCTCAGCGTCGTGCTCGTCCAC
GCGATCGAGCGGGCCAGGATCACCGGCCGCCGGCTGTTCGAGGTCTGCCTGACGATCCCC
GTCGCGGTGCCGGGCCTGGTGCTGGGCGTCGGGCTGCTCACCCTGCTGCTCAAGACCTCG
CTGTTCGGCACGCTGTGGATCATCGGCGTCGCCTACGTCGTACGGTTCCTGCCGTTCGCC
CATCGCTCGGTGTCGGCGGCGTTCCTGTCGGTGCACACCGAACTGGAGGAGGCCGCCCGC
ACCAGCGGGGCGTCCCTGGGGCGCTACGTCTTCCGGGTGCTGCTGCCGCTGGTCCGCCCG
GGACTGGTGTCCGGATGGCTGCTGGTCTTCCTCACCTTCATGCGCGAACTGCCGATGTCG
GCCCTGCTGCAGACGACCGGGACACAGACGCTCTCGGTGGCCCTGCTCAACAGCCGGTCC
TTCGACTCCCCGGGCGTGACGGCCGCGTTCACCCTGCTGCAGGCGGCCGTCCTGCTGGTG
GCCGCGGCGGTCTTCTGGACGATCACCACGCGCCGCGACGGCGCCTCCTCCGACATGGCG
GCCGGACTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000515 Binding-protein-dependent transport systems inner membrane component (Family) PS50928 ABC_TM1 PF00528 BPD_transp_1
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	Transmembrane 12

Rever_00260 Rever_00280