Rever_00280 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	98,486 / 99,700 / + [in whole cluster] 98,486 / 99,700 / + [in contig]
Location	98486..99700 [in whole cluster] 98486..99700 [in contig]
Type	CDS
Length	1,215 bp (404 aa)

Annotation

Category
Product
Product (GenBank)	putative transporter
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf3
Reference	ACC G1UDV8 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative transporter]
MGRKTMKTSTGGRTAPAPSPAPAETAPPRRTGHLEVAGLTRDFGSGGVRDASFSVERGQL
VTLLGPSGCGKTTTLRCLAGIEVPDAGRVRLGDTTMTATAEGVHLRPAKRDIGMVFQSYA
LWPHMSVFENVAFPLRTRGERTAAIRPRVEEMLELVGLEGMGRRQPSQLSGGQQQRVALA
RALVYRPGLLLLDEPLSNLDAARRDAVRRDIGRLQRELAITAVYVTHDQDEAMTLSDRIL
VMRDGAIVQNGTPAELLAAPRSLFVAGLLGQINVLTGVLATERTRTGPNGTERVGGVDIA
LADGTTRLSARLVGPAAVGENVSVVWRPGDITIDTAADDPSAPDRVNALPGTVTAVRVGS
SVTEYDIDAGITTLRALGLGRATASVGSRVHVSCPVDRSISLTH

>[putative transporter]
ATGGGCAGGAAGACGATGAAGACATCCACCGGCGGGCGGACCGCGCCCGCGCCGTCCCCC
GCGCCGGCCGAGACCGCGCCGCCGCGTCGCACCGGACACCTGGAGGTCGCCGGACTCACC
CGGGACTTCGGTTCCGGCGGAGTGCGCGACGCCTCGTTCTCGGTCGAGCGCGGCCAACTG
GTCACGCTGCTGGGCCCCTCGGGCTGCGGCAAGACGACCACCCTGCGCTGCCTGGCCGGC
ATCGAGGTCCCCGACGCGGGCCGGGTCCGGCTCGGCGACACGACGATGACCGCCACGGCC
GAGGGGGTGCACCTGCGGCCGGCCAAGCGCGACATCGGCATGGTCTTCCAGTCCTACGCG
CTGTGGCCGCACATGAGCGTCTTCGAGAACGTCGCCTTCCCGCTGCGCACCCGCGGTGAG
CGCACCGCCGCCATCCGGCCCCGCGTCGAGGAGATGCTCGAACTGGTCGGGCTGGAGGGC
ATGGGCCGGCGCCAGCCCAGCCAGCTCAGCGGCGGCCAGCAGCAGCGCGTCGCCCTCGCC
CGCGCCCTGGTCTACCGCCCCGGGCTGCTGCTGCTCGACGAGCCGCTCAGCAACCTCGAC
GCGGCCCGGCGCGACGCCGTACGCCGGGACATCGGCCGGCTGCAACGGGAGCTGGCCATC
ACCGCCGTCTACGTCACCCACGACCAGGACGAGGCGATGACGCTCTCCGACCGCATCCTG
GTGATGCGGGACGGCGCCATCGTCCAGAACGGGACGCCGGCCGAACTCCTCGCCGCGCCC
CGGTCCCTGTTCGTCGCCGGCCTGCTCGGCCAGATCAACGTCCTCACCGGCGTGCTGGCC
ACCGAGCGGACCCGGACCGGACCGAACGGCACCGAACGCGTCGGCGGGGTGGACATCGCC
CTGGCCGACGGCACGACCCGCCTGTCGGCCCGCCTCGTCGGGCCGGCCGCGGTCGGCGAG
AACGTGTCGGTCGTGTGGCGCCCCGGCGACATCACGATCGACACCGCCGCGGACGACCCG
TCGGCGCCGGACCGCGTGAACGCGCTGCCCGGCACCGTCACCGCCGTCCGCGTCGGCAGC
TCGGTCACCGAGTACGACATCGACGCCGGGATCACCACCCTGCGGGCCCTCGGCTTGGGA
CGCGCGACCGCGTCCGTCGGCTCCCGCGTCCACGTGTCCTGTCCGGTGGACAGGTCCATA
TCCCTCACCCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003439 ABC transporter-like (Domain) PS50893 ABC_TRANSPORTER_2 PF00005 ABC_tran IPR003593 AAA+ ATPase domain (Domain) SM00382 AAA IPR008995 Molybdate/tungstate binding (Domain) SSF50331 MOP_like IPR017871 ABC transporter, conserved site (Conserved_site) PS00211 ABC_TRANSPORTER_1
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Rever_00270 Rever_00290