Rever_00290 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	99,718 / 100,890 / + [in whole cluster] 99,718 / 100,890 / + [in contig]
Location	99718..100890 [in whole cluster] 99718..100890 [in contig]
Type	CDS
Length	1,173 bp (390 aa)

Annotation

Category
Product
Product (GenBank)	putative peptidase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf4
Reference	ACC G1UDV9 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative peptidase]
MPFDTIVRSASTFGPMQTDWETRIDMDRLRTERLAKTRAAMAAADVDFLIELRAENARYS
TGIKRLYWPTIQLGGGPLAVLAAEGDSAIWIIDPDYAAKNIPWVPDDRFHTPYQMDMTQD
VNSFVDDLYHYFGSKIETARIAVDIWSPSMFDVLTRRLPNATFVNGQELMIGARQIKTAD
EIKALKIAYAISEAGMQAAVNILKPGVRECELVGECFRAMWDLGTETTQCSEVVNSGPGA
HPYRRFHSDRIVQHGDLVNMDFGGCFSGYFGDFCRAFVCGDKPTPAQVDLLKRAHELQYE
QLNALGPGVSPADICAKLGRRTLGHGIGISAFEGPHLRAQDDYELKPGMTFSVTSPPIGE
EGVGGVHLEDEIVITETGIDLYSTYPYTLG

>[putative peptidase]
ATGCCCTTCGACACGATCGTGCGATCCGCGAGCACCTTCGGCCCGATGCAGACCGACTGG
GAGACGCGCATCGACATGGACCGGCTGCGCACCGAGCGGCTGGCGAAGACCCGCGCGGCG
ATGGCGGCCGCCGACGTCGACTTCCTGATCGAACTGCGCGCCGAGAACGCCCGCTACAGC
ACCGGCATCAAGCGGCTGTACTGGCCGACCATCCAGTTGGGCGGCGGCCCGCTGGCCGTC
CTGGCCGCCGAGGGCGACTCGGCGATCTGGATCATCGACCCGGACTACGCGGCCAAGAAC
ATCCCCTGGGTGCCGGACGACCGGTTCCACACCCCGTACCAGATGGACATGACCCAGGAC
GTCAACTCCTTCGTCGACGACCTGTACCACTACTTCGGCTCGAAGATCGAGACCGCCCGC
ATCGCGGTGGACATCTGGTCGCCGTCCATGTTCGACGTGCTGACCAGGCGGCTGCCCAAC
GCCACCTTCGTCAACGGCCAGGAGCTGATGATCGGCGCCCGCCAGATCAAGACCGCCGAC
GAGATCAAGGCCCTGAAGATCGCCTACGCCATCTCCGAGGCCGGCATGCAGGCCGCGGTC
AACATCCTCAAGCCCGGCGTGCGCGAGTGCGAACTGGTCGGCGAGTGCTTCCGCGCCATG
TGGGACCTGGGCACCGAGACCACCCAGTGCTCGGAGGTCGTCAACTCCGGGCCGGGCGCG
CACCCCTACCGCCGCTTCCACAGCGACCGGATCGTGCAGCACGGCGACCTGGTCAACATG
GACTTCGGCGGCTGCTTCTCCGGCTACTTCGGCGACTTCTGCCGCGCCTTCGTCTGCGGC
GACAAGCCCACCCCCGCCCAGGTGGACCTGCTCAAGCGGGCCCACGAGTTGCAGTACGAG
CAGCTCAACGCGCTCGGCCCCGGCGTCAGCCCGGCCGACATCTGCGCCAAGCTCGGCCGC
AGGACCCTCGGTCACGGCATCGGCATCTCCGCCTTCGAGGGCCCGCACCTGCGCGCCCAG
GACGACTACGAGCTCAAGCCCGGCATGACCTTCTCCGTCACCAGCCCGCCCATCGGCGAG
GAGGGTGTCGGCGGCGTCCACCTGGAGGACGAGATCGTCATCACCGAGACCGGCATCGAC
CTGTACTCGACCTACCCCTACACGCTGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000587 Creatinase (Domain) PF01321 Creatinase_N IPR000994 Peptidase M24, structural domain (Domain) SSF55920 Peptidase_M24_cat_core G3DSA:3.90.230.10 Peptidase_M24_cat_core PF00557 Peptidase_M24
SignalP	No significant hit
TMHMM	No significant hit

Rever_00280 Rever_00300