Rever_00300 : CDS information

Location

Organism	Streptomyces sp.
Strain	SN-593
Entry name	Reveromycin
Contig	AB568601
Start / Stop / Direction	100,893 / 101,957 / + [in whole cluster] 100,893 / 101,957 / + [in contig]
Location	100893..101957 [in whole cluster] 100893..101957 [in contig]
Type	CDS
Length	1,065 bp (354 aa)

Annotation

Category
Product
Product (GenBank)	putative oxidoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)	orf5
Reference	ACC G1UDW0 PmId [21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011) comment Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

Sequence

>[putative oxidoreductase]
MSGNRTPAAGWLVVGVGRHVDRYGLPALARARGAAAVALCGTDPVRTADLAERHGVGRWG
ASVEELLGDPGVTHVYVCSRNERHERHVTLAAAAGKHVLCEKPLAQDPAAARRTVAACAR
YGVVLGTGFHLRHNAAHREARRLVADGAIGRPLWVDVSYLHQVGHGDSAARLAASRTVTT
PSKGAMAGTGAHAVDLAQWLAGDRVVRLSATLAELDPTPAGPQRVVQVSATTRGGVLVTL
TAGRTTHPANGLTVIGEAGRLTVSESIGNTGGGTLSLRSAQGSTDTVFPPHDVYAAQFEA
FAAATGGAAAAPFGASGDDGLSALSVAETVDRALAGPAATQAVDPPARPTRGTP

>[putative oxidoreductase]
TTGTCCGGCAACCGTACGCCGGCCGCCGGCTGGCTCGTCGTGGGCGTCGGCCGCCACGTG
GACCGCTACGGTCTGCCGGCGCTCGCCCGCGCCCGCGGCGCCGCGGCCGTGGCCCTGTGC
GGGACCGACCCTGTCCGCACCGCCGACCTGGCGGAGCGGCACGGGGTCGGCCGGTGGGGC
GCCTCGGTCGAGGAACTGCTCGGCGACCCCGGCGTCACCCACGTCTACGTCTGCTCCCGC
AACGAGCGGCACGAGCGGCACGTCACGCTCGCCGCCGCGGCCGGCAAGCACGTCCTGTGC
GAGAAGCCGCTCGCGCAGGACCCGGCCGCGGCCCGCCGCACCGTCGCGGCCTGCGCGCGG
TACGGCGTCGTCCTCGGCACCGGCTTCCACCTGCGGCACAACGCCGCGCACCGGGAGGCG
CGGCGGCTGGTGGCCGACGGCGCGATCGGCCGCCCGCTGTGGGTGGACGTGTCCTACCTG
CACCAGGTGGGACACGGCGACTCCGCCGCGCGGCTGGCCGCCTCGCGCACGGTCACCACC
CCCAGCAAGGGGGCGATGGCGGGAACCGGGGCCCACGCCGTGGACCTCGCGCAGTGGCTG
GCCGGCGACCGCGTCGTCCGGCTGTCGGCGACGCTCGCCGAACTCGACCCGACACCGGCC
GGGCCGCAGCGCGTGGTCCAGGTCAGCGCGACCACCCGCGGCGGGGTGCTGGTCACCCTC
ACCGCGGGACGCACGACCCACCCGGCCAACGGGCTGACCGTGATCGGCGAGGCGGGGCGG
CTGACGGTGTCGGAGTCGATCGGGAACACCGGCGGCGGCACGCTGTCGCTCCGGTCGGCG
CAGGGCAGCACCGACACCGTCTTCCCCCCGCACGACGTCTACGCGGCGCAGTTCGAGGCG
TTCGCCGCCGCCACCGGCGGCGCGGCCGCCGCCCCGTTCGGCGCGTCCGGCGACGACGGG
CTGTCGGCGCTGAGCGTCGCGGAGACCGTGGACCGGGCCCTGGCCGGACCAGCAGCTACG
CAGGCCGTCGATCCGCCGGCCCGCCCGACCCGAGGAACACCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000683 Oxidoreductase, N-terminal (Domain) PF01408 GFO_IDH_MocA IPR004104 Oxidoreductase, C-terminal (Domain) PF02894 GFO_IDH_MocA_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Rever_00290 Rever_00310