Rever_00300 Rever_00300   Rever_00320 Rever_00320

Rever_00310 : CDS information

close this sectionLocation

Organism
StrainSN-593
Entry nameReveromycin
Contig
Start / Stop / Direction101,954 / 102,976 / + [in whole cluster]
101,954 / 102,976 / + [in contig]
Location101954..102976 [in whole cluster]
101954..102976 [in contig]
TypeCDS
Length1,023 bp (340 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category
Product
Product (GenBank)putative dehydrogenase
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in reveromycin biosynthesis.
Note (GenBank)
  • orf6
Reference
ACC
PmId
[21642985] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. (Nat Chem Biol. , 2011)
comment
Δorf1及びΔorf(-1)はreveromycinの生産に影響しなかった。従って、これらの遺伝子はreveromycinの生産に関与していないと考えられる。また、これらの遺伝子の上流領域にあるorf(-2)-orf(-4)及び下流領域にあるorf(2)-orf(8)も、配列解析により、reveromycinの生合成に関与しないと予想される。

close this sectionSequence

selected fasta
>[putative dehydrogenase]
MTVVNTVITKKLGYLATTERMLAALGPVAYCPDQRPETVREHLADARIVVATKGVTIDDA
VLDAAPHVRLIAAPTAGFDWIDVEAATRRGIPVVANTGAAADAVAEFTLGAVLALTRRIV
AADRDLHGSADWGPVRDRYSRADQQIGSDLTSSTVAVVGLGHIGLKTAEKLAVLRPQSII
GYDPFVPAERARQAGVEVVGDLHELAARADIVLLHVPLTPQTTRLVDAAFLARLRPSALL
VNPSRGEVVDEPALVAALREGRLRGAALDVFEQEPLPADSPLRGMDNVVLTPHIGGVTAQ
SDETRAREIAERVLACVAGTRPVGLVNPEVWDTRLAATTD
selected fasta
>[putative dehydrogenase]
GTGACCGTTGTGAACACCGTCATCACCAAGAAACTGGGCTACCTCGCCACCACCGAGCGG
ATGCTCGCGGCGCTCGGCCCGGTCGCCTACTGCCCGGACCAGCGCCCGGAGACGGTGCGC
GAGCACCTCGCCGACGCCCGGATCGTCGTGGCCACCAAGGGCGTCACCATCGACGACGCC
GTCCTCGACGCGGCACCGCACGTCCGCCTCATCGCCGCGCCCACCGCCGGCTTCGACTGG
ATCGACGTCGAGGCGGCCACCCGCCGCGGCATCCCGGTCGTCGCCAACACCGGCGCGGCG
GCGGACGCGGTCGCCGAGTTCACGCTCGGCGCCGTGCTGGCCCTCACCCGCCGTATCGTG
GCCGCCGACCGCGACCTGCACGGCAGCGCCGACTGGGGCCCGGTGCGCGACCGCTACTCC
CGGGCCGACCAGCAGATCGGCTCCGACCTGACCAGCAGCACGGTGGCCGTCGTGGGGCTG
GGCCACATCGGGCTGAAGACCGCCGAGAAACTCGCGGTGCTGCGCCCGCAGTCGATCATC
GGGTACGACCCGTTCGTCCCGGCCGAGCGGGCCCGGCAGGCCGGCGTCGAGGTGGTCGGA
GACCTGCACGAACTCGCCGCCCGGGCCGACATCGTGCTGCTGCACGTCCCGCTGACGCCG
CAGACCACCCGGCTGGTCGACGCGGCCTTCCTGGCGAGGCTGCGGCCCTCCGCCCTGCTG
GTCAACCCCTCGCGCGGCGAGGTGGTCGACGAGCCCGCGCTGGTCGCCGCGCTGCGCGAG
GGCCGGCTGCGGGGCGCCGCGCTCGACGTGTTCGAGCAGGAGCCGCTGCCCGCTGACTCG
CCGCTGCGCGGCATGGACAACGTCGTGCTCACCCCGCACATCGGCGGCGTCACCGCGCAG
TCGGACGAGACCCGGGCCCGGGAGATCGCCGAGCGCGTCCTCGCCTGCGTCGCCGGCACG
CGACCGGTCGGCCTGGTCAACCCCGAGGTCTGGGACACCCGGCTCGCCGCCACCACTGAC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006139 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (Domain)
[27-327] 1.99999999999999e-21 PF00389
PF00389   2-Hacid_dh
IPR006140 D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (Domain)
[110-295] 2.89999999999999e-53 PF02826
PF02826   2-Hacid_dh_C
[234-250] PS00671
PS00671   D_2_HYDROXYACID_DH_3
[155-183] PS00065
PS00065   D_2_HYDROXYACID_DH_1
IPR016040 NAD(P)-binding domain (Domain)
[16-112] 7.1e-28 G3DSA:3.40.50.720 [113-296] 5.69999999999998e-58 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Rever_00300 Rever_00300   Rever_00320 Rever_00320
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