Mith_00020 Mith_00020   Mith_00040 Mith_00040

Mith_00030 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction4,347 / 5,129 / + [in whole cluster]
4,347 / 5,129 / + [in contig]
Location4347..5129 [in whole cluster]
4347..5129 [in contig]
TypeCDS
Length783 bp (260 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative type II thioesterase
Product (GenBank)putative thioesterase
Gene
Gene (GenBank)mtmZ
EC number
Keyword
  • type II thioesterase
Note
Note (GenBank)
Reference
ACC
PmId
[9882681] The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster. (J Bacteriol. , 1999)
comment
mithramycin産生株Streptomyces argillaceusの染色体のシークエンスから、orfAとmtmRを同定している論文。

DISCUSSIONの中でmtmZ (thioesterase) と mtmA (S-adenosylmethionine synthase)の間にTCGAG コンセンサス配列が存在するとの記述あり。これに関する引用はUnpublished resultsとされている。
Related Reference
ACC
Q6W5Q5
NITE
FR008_00110
PmId
[18836004] Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. (Appl Environ Microbiol. , 2008)
comment
Blast 55th, id32%, 4e-18
Streptomyces sp. FR-008_fscTE
FscTE

fscTEはputative type II thioesterase (TEII)をコードする。
fscTE deletionでFR-008/candicidin産生は約90%減。
type I thioesteraseを不活化させたとき、FscTEは成熟伸長したpolyketideの放出を代償することができなかった。

FscTE and TylOのHydrolytic activitiesは、
propionyl-S-N-acetylcysteamine > methylmalonyl-S-N-acetylcysteamine
acetyl-S-N-acetylcysteamine > malonyl-S-N-acetylcysteamine

したがって、TEII はacyl carrier proteins(ACP)に結合するnon-elongatable residuesを選択的に除去することにより、効果的なpolyketide生合成を維持することが結論付けられる。

close this sectionSequence

selected fasta
>putative type II thioesterase [putative thioesterase]
MAAAQPIRLYCFAHAGAGVSAFHGWAARVGPDVVPVPVLLPGRDRRRREPRVTTRAALLD
DVMRLFGGAPTAPFVLYGHSLGALVAYTVGRALHEAGRRGPALLAVGACPPPHLPPEPAY
ALGVPDAELLRTLDLMAVVPSGTPGTEAGGIWDRAVRPVLRDDLTLARALRTAACTSSPA
DPLTTPLLIVAADDDPMASPAVAEGWSRWSEGPTRLHTVSGGHFFVRGPELPRLLGQACR
ALRPARRTPRAAGPVTSTGN
selected fasta
>putative type II thioesterase [putative thioesterase]
ATGGCCGCCGCACAGCCGATACGTCTCTACTGTTTCGCGCATGCCGGGGCGGGCGTCTCT
GCCTTCCACGGGTGGGCGGCCCGCGTCGGCCCCGACGTGGTGCCCGTCCCGGTGCTGCTG
CCCGGCCGCGACCGACGCCGCCGCGAACCCCGGGTCACCACCCGCGCGGCACTGCTCGAC
GATGTGATGCGGCTGTTCGGCGGCGCCCCGACGGCCCCGTTCGTCCTGTACGGACACAGC
CTCGGCGCCCTGGTCGCGTACACCGTCGGCCGCGCCCTGCACGAGGCGGGCCGGCGCGGC
CCCGCACTGCTCGCGGTCGGCGCCTGCCCGCCGCCTCACCTGCCGCCCGAACCGGCGTAC
GCACTCGGCGTGCCCGATGCCGAACTCCTGCGCACGCTGGACCTGATGGCCGTCGTGCCG
TCCGGCACCCCCGGCACCGAAGCCGGCGGAATCTGGGACCGTGCCGTACGGCCGGTGCTG
CGTGACGACCTGACACTCGCCCGCGCCCTGCGCACCGCGGCGTGTACATCCTCACCGGCG
GACCCGCTGACCACGCCGCTGCTCATCGTCGCCGCGGACGACGACCCGATGGCGTCACCC
GCGGTCGCGGAGGGCTGGAGCCGCTGGAGCGAGGGGCCCACGCGCCTGCACACGGTCTCC
GGGGGCCACTTCTTCGTCCGCGGCCCGGAACTGCCTCGGCTCCTCGGTCAGGCATGCCGC
GCGCTCCGCCCAGCGAGGCGCACGCCCCGCGCGGCAGGGCCGGTGACCAGCACCGGGAAC
TAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[8-228] 3.7e-24 PF00975
PF00975   Thioesterase
SignalP No significant hit
TMHMM No significant hit
Mith_00020 Mith_00020   Mith_00040 Mith_00040
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