Mith_00040 : CDS information

Location

Organism	Streptomyces argillaceus
Strain	ATCC 12956
Entry name	Mithramycin
Contig	X89899
Start / Stop / Direction	5,258 / 6,640 / + [in whole cluster] 5,258 / 6,640 / + [in contig]
Location	5258..6640 [in whole cluster] 5258..6640 [in contig]
Type	CDS
Length	1,383 bp (460 aa)

Annotation

Category	5.1 general function
Product	putative 5,10-methylenetetrahydrofolate reductase
Product (GenBank)	AdoMet synthetase and MTHF reductase fusion
Gene
Gene (GenBank)	mtmA
EC number
Keyword	SAM
Note	Contains S-adenosylmethionine synthetase partial domain at N-terminus.
Note (GenBank)
Reference	ACC Q194R6 PmId [9882681] The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster. (J Bacteriol. , 1999) comment mithramycin産生株Streptomyces argillaceusの染色体のシークエンスから、orfAとmtmRを同定している論文。 DISCUSSIONの中でmtmZ (thioesterase) と mtmA (S-adenosylmethionine synthase)の間にTCGAG コンセンサス配列が存在するとの記述あり。これに関する引用はUnpublished resultsとされている。
Related Reference	ACC O54235 PmId [9515933] ( , ) comment Blast 15th, id56%, 2e-86 Streptomyces lividans_metF 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) 腸内細菌において、methionineのmethyl基は、5,10-methylenetetrahydrofolate reductaseによってN5,10-methylenetetrahydrofolateから合成される5-methyltetrahydrofolateにより提供される。 5,10-methylenetetrahydrofolate reductaseをコードするStreptomyces lividans metFを破壊した株はmethionine要求性になったことから、streptomycetesでも腸内細菌でのようにfolate branchがmethionine生合成に必須であると示された。

Sequence

>putative 5,10-methylenetetrahydrofolate reductase [AdoMet synthetase and MTHF reductase fusion]
MSGHLFTSESVTEGHPDKIADRISGTIPDALLREDPRSRVAVETLITTGQVYVADKVTTG
AYVPIAQLVRDAVLTIGYDSSAKGFDGACCGVSVSIGPQSPDIAQGVDTAYESLVEGAAA
GADIDELDRQDAGDQGLMFGCATDESPNLMPLPIELAYRLARRLTDVRRNGLPPTFARTA
RPGSPEDRAGRARVLWDTIRRGEALRPDFVSVTYEAGGSPRDRTVALTRRIAAETTLRPV
AHLTAVGHSVAELRQIIGRYADVGIRDVFALRGDPPGDPRGPWSPHFEGFTYAHELVEPV
RSLGDFTIRMAAFPERHPRSPDWESDIRHFVAKCRAGADYAITQMFFGPDGYLRLRDPAA
AAGCHIPIIPEIMPAMDVRQIRRFAELSAVAFTEDLAHRLEQARGNPAVGHRIGVDHATA
MAERLLAEGVPGLHCITLNRSTAALDIHRNLRTSRSLSVR

>putative 5,10-methylenetetrahydrofolate reductase [AdoMet synthetase and MTHF reductase fusion]
ATGTCCGGCCATCTGTTCACCTCGGAGTCCGTCACCGAGGGACACCCCGACAAGATCGCC
GACCGCATCAGCGGCACGATCCCCGACGCCCTGCTGCGCGAGGACCCCCGCTCCCGGGTC
GCCGTGGAAACGCTGATCACAACCGGCCAGGTGTACGTCGCCGATAAGGTCACCACCGGC
GCGTACGTGCCGATCGCCCAACTGGTGCGAGACGCCGTGCTCACCATCGGTTACGACTCC
TCCGCCAAGGGCTTCGACGGCGCCTGCTGCGGCGTGTCGGTGTCGATCGGCCCGCAGTCC
CCGGACATCGCGCAGGGTGTCGACACGGCGTACGAGTCCCTGGTCGAGGGCGCAGCCGCA
GGTGCGGACATCGACGAGCTGGACCGGCAGGATGCCGGCGACCAGGGCCTGATGTTCGGC
TGCGCGACCGACGAGTCCCCGAACCTGATGCCGTTGCCCATCGAACTGGCGTACCGGCTC
GCCCGCCGCCTCACCGACGTCCGCCGGAACGGACTCCCCCCTACCTTCGCCCGGACGGCA
AGACCCGGGTCACCCGAGGACCGAGCAGGGCGAGCGCGCGTGCTCTGGGACACGATCCGC
CGGGGGGAGGCGCTGCGCCCCGACTTCGTCTCCGTCACGTACGAAGCGGGCGGTTCGCCC
CGCGACCGCACGGTCGCCCTCACTCGGCGAATCGCCGCCGAGACCACTCTGAGGCCGGTC
GCCCACCTGACCGCCGTGGGCCACTCGGTCGCCGAACTGCGCCAGATCATCGGTCGGTAC
GCGGACGTCGGCATCCGTGACGTGTTCGCGTTGCGCGGGGACCCGCCCGGCGATCCCCGG
GGCCCGTGGAGCCCGCACTTTGAGGGATTCACCTACGCCCACGAGCTGGTGGAACCGGTC
CGCTCGCTGGGGGACTTCACCATCAGAATGGCCGCCTTCCCCGAGCGGCATCCGCGGTCG
CCGGACTGGGAGAGCGACATCCGGCACTTCGTCGCCAAGTGCCGGGCCGGCGCCGACTAC
GCCATCACTCAGATGTTCTTCGGCCCGGACGGCTATCTGCGGCTGCGCGACCCGGCCGCG
GCGGCCGGGTGCCACATTCCGATCATCCCGGAGATCATGCCGGCCATGGACGTCCGCCAG
ATCCGCCGCTTCGCCGAACTCTCCGCTGTCGCCTTCACCGAAGACCTGGCGCACCGTCTG
GAGCAGGCGCGGGGCAACCCCGCCGTGGGTCACCGCATCGGTGTCGACCACGCCACCGCG
ATGGCCGAGCGCCTGCTCGCCGAGGGAGTACCGGGGCTGCACTGCATCACGCTCAACCGC
TCCACCGCCGCGCTCGACATCCACCGCAACCTTCGGACCTCAAGGAGCCTCAGTGTCCGC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003171 Methylenetetrahydrofolate reductase (Family) PF02219 MTHFR IPR022628 S-adenosylmethionine synthetase, N-terminal (Domain) PF00438 S-AdoMet_synt_N IPR022629 S-adenosylmethionine synthetase, central domain (Domain) PF02772 S-AdoMet_synt_M IPR022636 S-adenosylmethionine synthetase superfamily (Domain) SSF55973 S-AdoMet_synt
SignalP	No significant hit
TMHMM	No significant hit

Mith_00030 Mith_00050