Mith_00130 Mith_00130   Mith_00150 Mith_00150

Mith_00140 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction16,658 / 17,926 / + [in whole cluster]
16,658 / 17,926 / + [in contig]
Location16658..17926 [in whole cluster]
16658..17926 [in contig]
TypeCDS
Length1,269 bp (422 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)ketoacyl synthase
Gene
Gene (GenBank)mtmP
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[8654997] Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin. (Gene. , 1996)
comment
Streptomyces argillaceus ATCC 12956での5.3kb fragmentのシークエンスから6ORFsを同定。
この領域の2カ所の挿入不活化実験により、mithramycin生合成への関与を確認している。
mithramycin生合成は、type II PKSがコードする遺伝子の典型的なセットに関与する。

mtmP(422aa): KS

mtmPのC末とmtmKのN末を含むfragmentがAm(R) cassetteで置換されると、mithramycin非産生となることが確認されている。
Related Reference
ACC
Q54495
NITE
Nogl_00370
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
comment
Blast 11th, id74%, 1e-175
Streptomyces nogalater_snoa1
Snoa1
[DoBISCUIT]polyketide synthase beta-ketoacyl synthase subunit

S. nogalaterのanthracycline生合成gene clusterを解析。
11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。

sno1: KS, AT

aclacinomycin生合成がブロックされたS.galilaeus mutantsの相補によりminimal PKSを確認している。
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 12th, id72%, 1e-173
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS3..374

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketoacyl synthase]
MNRRVVITGIGVVAPGAVGVEAFWEQLTAGRTATRTISLFDASAFRSRIAAEVDFDAARH
GFGPAEAERLDRATQFALVSAREAVADAGLDGKTDPSRTGVALGSAVGCTTGLDTQYNVV
SEGGSDWYVDHTRAVDHLFDYFVPSSMAAEVAWSIGAQGPVALISTGCTSGLDSLGHAVA
LIREGSADVMVAGATEAPISPITVACFDAIKATSPRNDDPATASRPFDRSRNGFVLGEGS
AVLILESLENARRRDAHVYAEISGFASRCNAYHMTGLRPDGIEMAEAIRTALDEARLDPT
AVDYVNAHGSGTKQNDRHETAAFKRSLKDHAYRVPVSSIKSMIGHSLGAIGSLEVAASAL
AIEHDTVPPTANLHDRDPACDLDYTPITAREQRTDTVLSVGSGFGGFQSAMVLTAPGLRE
AA
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketoacyl synthase]
ATGAACCGTCGCGTCGTCATCACCGGCATCGGGGTCGTGGCCCCGGGGGCGGTGGGAGTC
GAGGCGTTCTGGGAGCAGCTCACCGCGGGCCGTACGGCCACCCGGACGATCAGTCTCTTC
GACGCCTCCGCGTTCCGCTCCCGGATCGCTGCCGAAGTGGACTTCGACGCGGCCCGGCAC
GGCTTCGGCCCCGCCGAGGCCGAACGCCTCGACCGGGCCACCCAGTTCGCCCTCGTCAGT
GCCCGCGAGGCCGTCGCCGACGCCGGCCTCGACGGCAAGACCGACCCGTCGCGCACCGGC
GTGGCGCTCGGCAGCGCGGTCGGCTGTACGACGGGTCTGGACACCCAGTACAACGTCGTG
AGCGAGGGCGGCTCCGACTGGTACGTCGACCACACCCGCGCGGTCGACCATCTCTTCGAC
TACTTCGTGCCCAGCTCCATGGCCGCCGAGGTCGCCTGGAGCATCGGTGCGCAGGGCCCC
GTGGCGCTGATCTCCACGGGCTGCACCTCCGGTCTCGACTCCCTCGGTCACGCCGTCGCG
CTGATCCGGGAGGGCAGTGCGGACGTGATGGTCGCAGGCGCGACCGAGGCACCGATCTCA
CCGATCACCGTCGCCTGCTTCGACGCCATCAAGGCCACCTCGCCGCGCAACGACGACCCG
GCCACCGCGTCCCGGCCGTTCGACCGCAGCCGCAACGGCTTCGTGCTCGGGGAGGGCAGC
GCCGTGCTCATCCTGGAGAGCCTCGAAAACGCCCGCCGCCGCGACGCCCACGTCTACGCC
GAGATCTCGGGGTTCGCCAGTCGCTGCAACGCGTACCACATGACGGGGCTGCGGCCCGAC
GGCATCGAGATGGCGGAGGCGATCCGCACCGCTCTCGACGAGGCACGCCTGGACCCCACG
GCCGTCGACTACGTCAACGCGCACGGCTCCGGCACCAAGCAGAACGACCGGCACGAGACC
GCCGCCTTCAAGCGCAGTCTCAAGGACCACGCCTACCGGGTCCCGGTGAGTTCCATCAAG
TCGATGATCGGGCACTCGCTCGGTGCCATCGGCTCCCTGGAGGTGGCCGCCAGCGCGCTG
GCCATCGAGCACGACACGGTACCTCCGACGGCCAACCTGCACGACCGGGACCCGGCCTGC
GACCTCGACTACACCCCGATCACCGCCCGTGAACAGCGCACCGACACCGTCCTGAGCGTG
GGCAGCGGATTCGGCGGCTTCCAGAGCGCCATGGTGCTGACGGCGCCCGGACTCCGGGAG
GCGGCATGA
KS3..374
KS7..1122

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-250] 8.30000000000004e-61 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[259-374] 1.6e-39 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[7-264] 1.20000000000001e-66 G3DSA:3.40.47.10 [269-415] 9.9e-51 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-415] 2.80000504261099e-68 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[159-175] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP No significant hit
TMHMM No significant hit
Mith_00130 Mith_00130   Mith_00150 Mith_00150
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