Mith_00140 Mith_00140   Mith_00160 Mith_00160

Mith_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction17,923 / 19,149 / + [in whole cluster]
17,923 / 19,149 / + [in contig]
Location17923..19149 [in whole cluster]
17923..19149 [in contig]
TypeCDS
Length1,227 bp (408 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)mtmK protein
Gene
Gene (GenBank)mtmK
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[8654997] Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin. (Gene. , 1996)
comment
Streptomyces argillaceus ATCC 12956での5.3kb fragmentのシークエンスから6ORFsを同定。
この領域の2カ所の挿入不活化実験により、mithramycin生合成への関与を確認している。
mithramycin生合成は、type II PKSがコードする遺伝子の典型的なセットに関与する。

mtmK(408aa): CLF

mtmPのC末とmtmKのN末を含むfragmentがAm(R) cassetteで置換されると、mithramycin非産生となることが確認されている。
Related Reference
ACC
Q54496
NITE
Nogl_00380
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
comment
Blast 11th, id66%, 1e-134
Streptomyces nogalater_snoa2
Snoa2
[DoBISCUIT]polyketide synthase chain length factor subunit

S. nogalaterのanthracycline生合成gene clusterを解析。
11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。

sno2: CLF

aclacinomycin生合成がブロックされたS.galilaeus mutantsの相補によりminimal PKSを確認している。
ACC
Q54174
NITE
Urd_00130
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 9th, id63%, 1e-135
Streptomyces fradiae_urdB
Putative chain length determinant

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。
UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

CLF7..364

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [mtmK protein]
MSADASQAVITGIGVAAPNGLSVKAWWDAVLRGESGIRRLSRFDPGRYPARLAGEIRDFV
DADHVPGRLLPQTDRVTRLSLAVAREAVEDAGVDLERLPRYAAGVSTASSAGGFEFGQRE
LQALWSKGGQYVSAYQSFAWFYAVNTGQISIGYDLRGPSGVLVTEQAGGLDAVAQARRLL
RRGSELMVTGGVDSALCPWGWTAHLAGGRLSTGDDPARAYLPFSADADGEVVGEGGALLV
LERAAAAPSRGARVYGVFAGYAATFDPPPGRGGTPQLARAAELALVDAGMTPDQVDVVFA
DAAGERAADRAEADALTRVFGARGVPVTAPKTMTGRLSAGGASLDLAAALLALRDQVVPP
TVNVTEPADDCPVDLVTGRPRPLPLRAALVLARGRGGFNAAAVVRALS
selected fasta
>polyketide synthase chain length factor subunit [mtmK protein]
ATGAGCGCCGACGCCTCGCAGGCGGTCATCACCGGCATCGGTGTCGCCGCGCCCAACGGG
CTGTCCGTCAAGGCCTGGTGGGACGCCGTGCTGAGGGGTGAGAGCGGGATCCGCAGGCTC
AGCCGGTTCGACCCCGGCCGCTATCCGGCACGGCTCGCCGGCGAGATCCGCGACTTCGTG
GACGCCGACCACGTTCCGGGCCGGCTGCTGCCCCAGACCGACCGGGTGACCAGGCTGTCC
CTCGCCGTGGCCCGGGAGGCCGTCGAGGACGCGGGCGTCGACCTGGAGCGGCTGCCCCGG
TACGCGGCCGGTGTCAGCACCGCGAGCTCGGCGGGCGGGTTCGAGTTCGGGCAGCGGGAG
CTGCAGGCCCTGTGGAGCAAAGGCGGGCAGTACGTCAGCGCGTACCAGTCGTTCGCCTGG
TTCTACGCCGTCAACACCGGGCAGATCTCGATCGGCTACGACCTGCGCGGTCCCAGCGGC
GTGCTGGTCACCGAGCAGGCCGGGGGGCTCGACGCGGTCGCCCAGGCCCGCCGCCTGCTG
CGCCGGGGCAGCGAGCTGATGGTCACCGGAGGCGTCGACTCCGCGCTGTGCCCCTGGGGC
TGGACCGCCCATCTGGCGGGCGGCCGGCTGTCCACCGGCGACGACCCGGCGCGTGCCTAC
CTGCCGTTCTCGGCGGACGCCGACGGGGAGGTCGTCGGTGAGGGAGGCGCCCTCCTGGTG
CTGGAGCGGGCCGCCGCGGCCCCGTCCCGCGGCGCCCGCGTCTACGGTGTCTTCGCCGGG
TACGCGGCCACCTTCGACCCGCCGCCCGGGCGGGGCGGCACCCCGCAGCTCGCCCGGGCG
GCCGAACTCGCCCTGGTCGACGCCGGGATGACCCCGGACCAGGTGGACGTCGTGTTCGCC
GACGCCGCGGGCGAGCGCGCCGCCGACCGAGCCGAGGCCGATGCCCTGACCCGGGTGTTC
GGCGCCCGTGGGGTGCCGGTCACCGCGCCGAAGACCATGACCGGGCGGCTCTCGGCCGGC
GGCGCCTCCCTGGACCTGGCGGCCGCCCTGCTGGCACTGCGCGACCAGGTCGTACCGCCC
ACCGTGAACGTCACCGAACCCGCCGACGACTGTCCCGTCGACCTGGTCACCGGCCGCCCC
CGGCCGCTGCCGCTGCGCGCCGCCCTCGTGCTGGCCAGGGGCCGCGGCGGATTCAATGCC
GCGGCCGTCGTGCGGGCCTTGTCCTGA
CLF7..364
CLF19..1092

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[7-245] 1.40000000000001e-53 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[255-364] 4.2e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[6-259] 1.5e-55 G3DSA:3.40.47.10 [275-406] 1.09999999999999e-39 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[5-252] 1e-61 SSF53901 [215-407] 1.3000049540733e-48 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Mith_00140 Mith_00140   Mith_00160 Mith_00160
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