Mith_00270 : CDS information

Location

Organism	Streptomyces argillaceus
Strain	ATCC 12956
Entry name	Mithramycin
Contig	X89899
Start / Stop / Direction	30,885 / 31,865 / + [in whole cluster] 30,885 / 31,865 / + [in contig]
Location	30885..31865 [in whole cluster] 30885..31865 [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	3.2 modification methylation
Product	C-methyltransferase
Product (GenBank)	C-methyltransferase
Gene
Gene (GenBank)	mtmMII
EC number	2.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q194P8 PmId [10652287] Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus. (J Biol Chem. , 2000) [14660589] MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction. (J Biol Chem. , 2004) comment [PMID:10652287](2000) 不活化mutant作成、異種性発現・精製・活性測定により、MtmMI は4-demethylpremithramycinoneの4-O-methylationを、MtmMII は9-demethylpremithramycin A3の9-C-methylationを担うことが実証されている。 -- [PMID:14660589](2004) MtmMII が担う芳香環methyl化と生物学的活性との関連を調査。

Sequence

>C-methyltransferase [C-methyltransferase]
MAQAVRRAGVLCAGMDLAVFDTMADGPADAETIAARLDVPVRGARVLLRALAAIDLLKTD
GERFWLAPGVDRVLVRSSPGWFGDILRHAVSRYEWDALGRLSDAVRRGGTVLDTNAETPD
YPFWADLARTPTPHTPLVADLLADTLEPWAAGRERLDVLDVACGHGLYGYTVARRYPQAR
VRSVDWPGVLEHSRGHAERLGVLDRVELTPGDMFDVPLGGPYDLSLITNVLHHFSAERAG
EMLRRLAEATRPGGRIGIVAVTADGRDPASDPVPHLFAALMLAWTHEGESHALETYRDLL
TANGFGEPRLHEVGGVPLRLLLAERL

>C-methyltransferase [C-methyltransferase]
ATGGCCCAGGCGGTGCGCCGCGCCGGAGTGTTGTGTGCCGGGATGGACCTCGCCGTCTTC
GACACGATGGCGGACGGACCGGCCGACGCCGAGACCATCGCCGCCCGGCTGGACGTGCCG
GTCCGGGGCGCGCGGGTCCTGCTGCGGGCGCTCGCCGCGATCGACCTTCTGAAGACGGAC
GGCGAGCGTTTCTGGCTGGCCCCCGGAGTCGACCGGGTCCTGGTCCGGTCCAGCCCCGGC
TGGTTCGGCGACATCCTCCGGCACGCGGTCAGCCGTTACGAGTGGGATGCCCTGGGCCGC
CTCTCGGACGCGGTCCGGCGCGGCGGAACGGTGCTGGACACCAACGCCGAGACCCCCGAC
TACCCGTTCTGGGCGGACCTGGCCCGCACCCCCACCCCGCACACCCCCCTGGTCGCCGAT
CTACTGGCCGACACCCTGGAGCCGTGGGCGGCGGGCCGCGAGCGCCTGGACGTCCTGGAC
GTGGCCTGCGGGCACGGGCTGTACGGCTACACGGTGGCTCGGCGCTATCCGCAGGCGCGG
GTCCGTTCCGTCGACTGGCCGGGCGTCCTGGAGCACTCCCGGGGCCACGCGGAGCGCCTG
GGCGTGCTGGACCGGGTCGAACTGACCCCGGGCGACATGTTCGACGTGCCGCTGGGCGGC
CCGTACGACCTGTCGCTGATCACCAATGTGCTGCACCACTTCTCCGCCGAGCGTGCGGGC
GAGATGCTGCGGCGCCTGGCGGAGGCGACCCGGCCGGGCGGGCGGATCGGCATCGTCGCG
GTCACCGCGGACGGCCGTGACCCGGCGTCCGACCCGGTCCCCCACCTGTTCGCCGCGTTG
ATGTTGGCCTGGACCCACGAAGGCGAGTCGCACGCGCTGGAGACCTACCGGGACCTGCTG
ACGGCCAACGGTTTCGGGGAGCCCCGGCTGCACGAGGTGGGCGGGGTGCCGCTGCGCCTG
CTGCTCGCAGAGCGGCTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001077 O-methyltransferase, family 2 (Domain) PF00891 Methyltransf_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	No significant hit
TMHMM	No significant hit

Mith_00260 Mith_00280