Mith_00290 Mith_00290   Mith_00310 Mith_00310

Mith_00300 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction34,474 / 35,655 / + [in whole cluster]
34,474 / 35,655 / + [in contig]
Location34474..35655 [in whole cluster]
34474..35655 [in contig]
TypeCDS
Length1,182 bp (393 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductD-olivose glycosyltransferase
Product (GenBank)glycosyltransferase
Gene
Gene (GenBank)mtmGI
EC number
Keyword
  • disaccharide 1st D-olivose
Note
Note (GenBank)
Reference
ACC
PmId
[9733697] Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin. (J Bacteriol. , 1998)
comment
mithramycinに抵抗性を与えるcosmid clone (cosAR3)からmtmGI and mtmGII を同定。
両遺伝子の推定産物は、抗生物質生合成でのglycosyl transferに関与するglycosyltransferasesを含むglycosyltransferases and glucuronosyltransferasesと類似性を示した。

両遺伝子の不活化mutantsはmithramycin非産生で、disaccharide部分を持たない化合物を蓄積。
よって、mtmGI and mtmGII によってコードされるglycosyltransferasesはmithramycin生合成の際にdisaccharideを形成、移動することを担うと提唱される。

また、蓄積した新規代謝産物の構造から、aglyconeの最終成形(4環→3環)の前にtrisaccharide glycosyl transfer stepsが含まれると示唆された。

close this sectionSequence

selected fasta
>D-olivose glycosyltransferase [glycosyltransferase]
MRILFVASVSASHLAPMVPLAWALRSAGHDVRVACDADTAPGALALGLTVVRVSTDGDFA
RRHRESVRGVPGEPYYRERDALPRMFADRSLAMLDGLAALVDRWRPHAVVHEPVAFAAET
VAAARDIPTLRHLWGPDLFGTPPGAWLRHRVRELVTAEVPDSAGASAREVVIDPCPAPLQ
RPGADAVVPVRYVPVDRPGTVPAWLLEPPRGPRVCVTWGTFADGMPGGHPLMRAMRALPR
LGVEVVLVARAEDLARQGGLPAGVRAVTGAPLHAVLPSCTAVVHHGGSNTALGAVVRGLP
QLVVSDTFERDLNAQRLAETGAAVHLEAREAEADPDAVATAVGRLVDDDRYARAAAELRG
LALTRPSPARVAGGFEELLTQWPVAGAPITEGA
selected fasta
>D-olivose glycosyltransferase [glycosyltransferase]
ATGCGCATCTTGTTCGTGGCCTCCGTCTCCGCCTCGCACCTCGCTCCCATGGTTCCGCTG
GCCTGGGCCCTGCGGTCCGCGGGGCACGACGTGCGGGTGGCCTGTGACGCCGACACGGCA
CCGGGTGCGCTGGCGCTCGGGCTGACCGTGGTGCGGGTGAGCACGGACGGCGACTTCGCC
CGGCGGCACCGCGAGAGCGTGCGGGGCGTGCCCGGCGAGCCGTACTACCGGGAGCGGGAC
GCGCTGCCCCGCATGTTCGCCGACCGGTCCCTCGCCATGCTCGACGGTCTGGCGGCACTC
GTCGACCGCTGGCGGCCGCACGCGGTGGTGCACGAGCCGGTGGCCTTCGCCGCGGAAACG
GTCGCCGCCGCACGGGACATCCCCACGCTGCGGCACCTCTGGGGCCCTGACCTCTTCGGT
ACGCCGCCCGGCGCCTGGCTGCGGCACCGGGTGCGCGAGCTGGTGACGGCCGAGGTGCCG
GACAGCGCGGGGGCCTCGGCGCGGGAGGTCGTGATCGACCCCTGTCCCGCTCCGCTGCAG
CGACCGGGGGCCGACGCCGTCGTCCCGGTCCGCTACGTCCCCGTGGACCGGCCGGGAACG
GTCCCGGCCTGGCTGCTGGAACCGCCCCGAGGGCCGCGGGTCTGTGTCACCTGGGGGACG
TTCGCCGACGGCATGCCGGGCGGCCATCCGCTGATGCGGGCGATGCGCGCGCTGCCGCGG
CTCGGCGTGGAGGTGGTGCTCGTCGCGCGCGCCGAGGACCTGGCCCGGCAGGGCGGACTC
CCCGCCGGTGTGCGGGCGGTGACCGGCGCGCCCCTGCACGCCGTCCTGCCCTCGTGCACG
GCGGTCGTGCACCACGGCGGGTCCAACACCGCGCTGGGTGCCGTGGTCCGCGGACTGCCG
CAGCTCGTCGTGAGCGACACCTTCGAACGAGATCTGAACGCGCAGCGGCTGGCGGAGACC
GGTGCGGCGGTGCACCTGGAAGCACGGGAGGCGGAGGCGGATCCGGACGCCGTCGCGACG
GCGGTGGGACGGCTGGTTGACGACGACCGGTACGCGCGGGCCGCCGCGGAACTGCGCGGC
CTGGCCCTGACCCGCCCGTCCCCGGCCCGGGTCGCCGGTGGCTTCGAGGAACTGCTCACC
CAGTGGCCCGTAGCGGGCGCACCGATCACGGAAGGCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-136] 2e-09 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[172-265] 3.3e-20 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.501 Signal
Bacteria, Gram-positive   
[1-23] 0.453 Signal
Eukaryota   
TMHMM No significant hit
Mith_00290 Mith_00290   Mith_00310 Mith_00310
Page top