Mith_00320 Mith_00320   Mith_00340 Mith_00340

Mith_00330 : CDS information

close this sectionLocation

Organism
StrainATCC 12956
Entry nameMithramycin
Contig
Start / Stop / Direction39,841 / 40,500 / + [in whole cluster]
39,841 / 40,500 / + [in contig]
Location39841..40500 [in whole cluster]
39841..40500 [in contig]
TypeCDS
Length660 bp (219 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative PadR family transcriptional regulator
Product (GenBank)MtrY protein
Gene
Gene (GenBank)mtrY
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[8757400] An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus. (Mol Gen Genet. , 1996)
[10779713] Insertional inactivation of mtrX and mtrY genes from the mithramycin gene cluster affects production and growth of the producer organism Streptomyces argillaceus. (FEMS Microbiol Lett. , 2000)
comment
[PMID:8757400](1996)
S.albusに外因性mithramycinへの抵抗性を与えるS. argillaceusの最小DNA領域(3.9kb)がシークエンス、配列解析され、不完全なORF(mtrX)と完全ORFs 3つ(mtrY, A, B)が予測された。

mtrYの推定産物は、DBに明らかな類似性を示すものなし。

mtrX と mtrYの各frameshift mutantは、外因性mithramycinに対する抵抗性に影響がない。しかし、内因性に合成されたmithramycinに対する防御と分泌に関与するかもしれないという可能性は残っている。

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[PMID:10779713](2000)
mtrYの遺伝子置換による不活化でmithramycin生合成は50%減。
mtrYを過剰発現するとmithramycin生合成は約47%増。

よって、mtrYはたぶんpositive regulatory elementをコードし、その発現は普通レベルのmithramycin産生に重要であると強く示唆される。

N末近くにはputative helix-turn-helix motif (HTH)あり。
Family/Domain
FD
IPR005149
comment
[IPR005149] Transcription regulator PadR N-terminal (Domain)

close this sectionSequence

selected fasta
>putative PadR family transcriptional regulator [MtrY protein]
MAVRKRPVSNLLGLAVLGLLLEHAMHPYEMAATLRERNKDTSFKVRTGSLYDVVEAWYGR
WIAEHSTERAGRRPERTVYAHTDLGREEFMSWLDELVRTPVKEYPSFLAAVSYLGALGPE
RAVQALRERIARLDEQIEEARRARTAVLDQQTPRLFVIELEYALTMAEAERDWARRIVAE
VHGGTLAWPELTTKEGREVWTDQDETEDKTDDRTEKQQV
selected fasta
>putative PadR family transcriptional regulator [MtrY protein]
ATGGCGGTGCGCAAACGGCCGGTGTCCAACCTGCTCGGGCTCGCGGTCCTCGGCCTGCTG
CTGGAGCACGCGATGCATCCGTACGAGATGGCCGCGACCCTGCGGGAGCGCAACAAGGAC
ACGAGCTTCAAGGTCAGGACCGGCTCGCTCTACGACGTCGTGGAGGCCTGGTACGGGCGC
TGGATCGCCGAGCACAGCACTGAACGTGCCGGACGCCGACCGGAGCGCACCGTGTACGCC
CATACCGACCTGGGCCGCGAGGAGTTCATGAGCTGGCTCGACGAACTGGTCCGCACCCCG
GTGAAGGAGTACCCCAGCTTCCTCGCCGCGGTGAGCTACCTCGGCGCCCTCGGGCCGGAA
CGCGCCGTCCAGGCCCTGCGGGAACGCATCGCCCGCCTGGACGAGCAGATCGAGGAGGCC
CGCCGGGCACGGACCGCGGTGCTGGACCAGCAGACGCCACGGCTGTTCGTGATCGAACTC
GAATACGCCCTGACCATGGCCGAAGCCGAGCGGGACTGGGCCCGGCGGATCGTCGCCGAA
GTCCACGGCGGCACCCTCGCCTGGCCCGAACTCACCACCAAGGAAGGCCGCGAGGTATGG
ACCGACCAGGACGAGACGGAAGACAAGACGGACGACAGGACGGAGAAGCAGCAGGTATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005149 Transcription regulator PadR N-terminal (Domain)
[16-89] 1.2e-06 PF03551
PF03551   PadR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[12-96] 3.2e-12 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP
[1-31] 0.55 Signal
Eukaryota   
TMHMM No significant hit
Mith_00320 Mith_00320   Mith_00340 Mith_00340
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