Oxtet_00160 Oxtet_00160   Oxtet_00180 Oxtet_00180

Oxtet_00170 : CDS information

close this sectionLocation

Organism
StrainATCC 10970 (=NBRC 12907)
Entry nameOxytetracycline
Contig
Start / Stop / Direction18,911 / 17,832 / - [in whole cluster]
18,911 / 17,832 / - [in contig]
Locationcomplement(17832..18911) [in whole cluster]
complement(17832..18911) [in contig]
TypeCDS
Length1,080 bp (359 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
ProductC-4 aminotransferase
Product (GenBank)OxyQ
GeneoxyQ
otcX2
Gene (GenBank)oxyQ
EC number2.6.1.-
Keyword
Note
Note (GenBank)
  • putative aminotransferase
Reference
ACC
PmId
[16597959] Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase. (Appl Environ Microbiol. , 2006)
[18422316] Identifying the minimal enzymes required for anhydrotetracycline biosynthesis. (J Am Chem Soc. , 2008)
comment
[PMID:16597959](2006)
oxytetracycline gene clusterのシークエンス、配列解析。
resistance genes otrAとotrBの間に全21genesを同定。
わかりやすくするため、gene namesがotc → oxyに変更され、振り直されている。

oxyQ (otcX2): Aminotransferase

アミノ酸代謝に関連するaspartate/tyrosine/aromatic aminotransferasesに相同であることから、pyridoxal 5'-phosphateをcofactorとして4-keto-ATCのC-4にアミノ基転移する酵素のようだ。

---
[PMID:18422316](2008)
完全に機能的なring Aを含む最初の中間体anhydrotetracycline (ATC)の生合成に必要な最小酵素セットの同定と再構築。

in vivo and in vitro approachesを組み合わせて、6-methylpretetramid → ATC への変換に必要なのはOxyL, OxyQ, OxyTであることを確認した。

OxyLはNADPH-dependent dioxygenaseであり、6-methylpretetramid に2つの酸素原子を導入し、不安定な中間体4-keto-ATC をもたらす。
aminotransferase OxyQは4-keto-ATC の還元的アミノ化を触媒し、4-amino-ATC をもたらす。
さらに、N,N-dimethyltransferase OxyTはSAM-dependent様式で、4-amino-ATC → ATC の形成を触媒する。

CH999/pWJ135(OxyABCDJKNF + OxyL)で、4-keto-ATCの分解産物を確認。
CH999/pWJ209(OxyABCDJKNF + OxyL + OxyQ)では、4-amino ATCを蓄積。
よってOxyQはreductive transaminaseであると確認された。

close this sectionSequence

selected fasta
>C-4 aminotransferase [OxyQ]
MRELPEFPWDVLLPYKKRAAAHPDGLVNLALGEPVDATPDVLRDALAAATDAPGYPPTEG
TPALREAAAAWLRRRLGVTVDPSAVLPAVGTKELIAWLPAMLGTGPGDTVAFPRLAFPTF
DVSARLAGARGRPVDSPLELGSEPVKVVWLNSPSNPEGRVLSVPELREIVAWARDRGAVL
VNDECYIEYGWDRRPVSLLDSAVCGGSHDGLLAVHSLSKRSNLAGYRAGVCSGDPALIGR
LLQVRKHAGHAVPAPVQAAMVAALEDDAHVERQRDRYAYRRRVLRTALEGAGFRVEHSEG
GLFLWATRGEPCWPAVQKLADLGILVAPGAFYGEAGEQYVRIAFTATDERIAAAAARLT
selected fasta
>C-4 aminotransferase [OxyQ]
ATGAGGGAACTGCCCGAATTTCCGTGGGATGTGCTGCTGCCGTACAAGAAGCGCGCCGCG
GCCCACCCCGACGGGCTGGTCAACCTGGCGCTGGGCGAACCGGTCGACGCCACGCCCGAC
GTGCTGCGCGACGCCCTCGCGGCGGCGACCGACGCGCCCGGCTACCCGCCCACGGAGGGG
ACGCCCGCGCTCCGCGAAGCGGCCGCCGCATGGCTGCGCCGCCGCCTGGGCGTGACGGTG
GACCCCTCGGCCGTACTGCCCGCGGTCGGCACCAAGGAGCTGATCGCCTGGCTCCCGGCG
ATGCTGGGCACCGGCCCCGGCGACACGGTGGCCTTTCCCCGGCTGGCGTTTCCCACCTTC
GACGTCAGCGCGCGCCTCGCGGGCGCGCGGGGGCGCCCGGTGGATTCACCGCTGGAGCTC
GGCAGTGAACCGGTCAAGGTGGTCTGGCTCAACTCGCCGTCCAACCCGGAGGGCCGGGTG
CTGTCCGTACCCGAGCTGCGCGAGATCGTGGCCTGGGCGCGGGACCGGGGCGCCGTCCTC
GTCAACGACGAGTGCTACATCGAATACGGGTGGGACCGGCGGCCGGTGTCGCTGCTGGAC
TCGGCGGTGTGCGGCGGCAGTCACGATGGTCTGCTGGCGGTGCACTCGCTGTCCAAGCGG
TCCAACCTCGCGGGCTACCGCGCGGGCGTCTGCTCCGGCGACCCGGCCCTGATCGGGCGC
CTCCTCCAGGTACGCAAGCACGCCGGGCACGCGGTCCCGGCCCCCGTACAGGCGGCCATG
GTGGCCGCCCTGGAGGACGACGCCCACGTGGAGCGGCAGCGAGACCGGTACGCCTACAGG
CGGCGCGTTCTGCGCACGGCCCTGGAGGGGGCCGGGTTCCGCGTCGAGCATTCCGAAGGC
GGCCTGTTCCTGTGGGCCACCCGGGGTGAGCCCTGCTGGCCCGCCGTACAGAAGCTGGCC
GATCTCGGCATCCTCGTCGCACCCGGTGCCTTCTACGGCGAGGCCGGTGAGCAGTACGTA
CGGATTGCGTTCACGGCGACCGACGAACGCATCGCCGCGGCCGCCGCCCGGCTGACATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001176 1-aminocyclopropane-1-carboxylate synthase (Family)
[107-128] 6.40000038788486e-05 PR00753 [142-166] 6.40000038788486e-05 PR00753 [210-234] 6.40000038788486e-05 PR00753
PR00753   ACCSYNTHASE
IPR004839 Aminotransferase, class I/classII (Domain)
[26-358] 3.89999999999997e-44 PF00155
PF00155   Aminotran_1_2
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[38-258] 9e-51 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[259-358] 7.90000000000001e-11 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[1-358] 1.90000694315261e-85 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
IPR019880 Succinyldiaminopimelate transaminase, DapC, actinobacteria (Family)
[4-359] TIGR03539
TIGR03539   DapC_actino
SignalP No significant hit
TMHMM No significant hit
Oxtet_00160 Oxtet_00160   Oxtet_00180 Oxtet_00180
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