Oxtet_00170 Oxtet_00170   Oxtet_00190 Oxtet_00190

Oxtet_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 10970 (=NBRC 12907)
Entry nameOxytetracycline
Contig
Start / Stop / Direction19,336 / 18,908 / - [in whole cluster]
19,336 / 18,908 / - [in contig]
Locationcomplement(18908..19336) [in whole cluster]
complement(18908..19336) [in contig]
TypeCDS
Length429 bp (142 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productpyridoxamine 5'-phosphate oxidase-like FMN-binding domain protein
Product (GenBank)OxyR
GeneoxyR
otcX1
Gene (GenBank)oxyR
EC number
Keyword
Note
Note (GenBank)
  • putative PNP-oxidase
Reference
ACC
PmId
[16597959] Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase. (Appl Environ Microbiol. , 2006)
[18422316] Identifying the minimal enzymes required for anhydrotetracycline biosynthesis. (J Am Chem Soc. , 2008)
comment
[PMID:16597959](2006)
oxytetracycline gene clusterのシークエンス、配列解析。
resistance genes otrAとotrBの間に全21genesを同定。
わかりやすくするため、gene namesがotc → oxyに変更され、振り直されている。

oxyR (otcX1): PNP-oxidase

putative pyridoxamine 5'-phosphate(PMP) oxidase OxyRは、cofactorとしてpyridoxal 5'-phosphate (PLP)を使うaminotransferase OxyQと一緒に翻訳される。
相同なActVA-ORF2はactinorhodin生合成に関連した機能不明な酵素である。

tableではPNP-、本文ではPMP-oxidaseとなっているが同じ酵素(EC 1.4.3.5)を表している。

---
[PMID:18422316](2008)
完全に機能的なring Aを含む最初の中間体anhydrotetracycline (ATC)の生合成に必要な最小酵素セットの同定と再構築。

中間体6-methylpretetramid → ATC の変換にoxygenase候補OxyG, OxyE, or OxyRがあってもなくても、その産物特性と収量に影響なし。
Family/Domain
FD
IPR011576
comment
[IPR011576] Pyridoxamine 5'-phosphate oxidase-like, FMN-binding domain (Domain)
function; pyridoxamine-phosphate oxidase activity, FMN binding

close this sectionSequence

selected fasta
>pyridoxamine 5'-phosphate oxidase-like FMN-binding domain protein [OxyR]
MPFTQKEITYLRAQGYGRLATVGAHGEPHNVPVSFEIDEERGTIEITGRDMGRSRKFRNV
AKSDRVAFVVDDVPCRDPEVVRAVVIHGTAQALPTGGRERRPHCADEMIRIHPRRIVTWG
IEGDLSTGVHARDITAEDGGRR
selected fasta
>pyridoxamine 5'-phosphate oxidase-like FMN-binding domain protein [OxyR]
ATGCCGTTCACCCAGAAGGAAATCACCTACCTGCGCGCGCAGGGCTACGGCCGTCTGGCG
ACGGTCGGCGCCCATGGAGAACCCCACAACGTGCCCGTCTCGTTCGAGATCGACGAGGAG
CGCGGCACCATCGAGATCACCGGCCGGGACATGGGCCGCAGCCGCAAGTTCCGGAACGTG
GCGAAGAGCGACCGGGTCGCCTTCGTCGTGGACGACGTGCCGTGCCGCGACCCCGAGGTG
GTCCGCGCCGTCGTCATCCACGGCACGGCCCAGGCGCTGCCCACCGGCGGCAGGGAGCGC
CGGCCGCACTGCGCCGACGAGATGATCCGCATCCACCCCCGGCGCATCGTCACCTGGGGC
ATCGAAGGGGACCTGTCCACCGGGGTCCACGCACGCGACATCACGGCCGAGGACGGAGGA
CGGCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009002 FMN-binding split barrel-related (Domain)
[3-124] 7.00000734129907e-21 SSF50475
SSF50475   FMN_binding
IPR011576 Pyridoxamine 5'-phosphate oxidase-like, FMN-binding domain (Domain)
[4-94] 6.60000000000002e-12 PF01243
PF01243   Pyridox_oxidase
IPR012349 FMN-binding split barrel (Domain)
[8-102] 1.99999999999999e-18 G3DSA:2.30.110.10
G3DSA:2.30.110.10   PNPOx_FMN_bd
IPR024031 F420-dependent enzyme, PPOX class, family MSMEG5819, probable (Family)
[3-121] 4.9e-41 TIGR04023
TIGR04023   PPOX_MSMEG_5819
SignalP No significant hit
TMHMM No significant hit
Oxtet_00170 Oxtet_00170   Oxtet_00190 Oxtet_00190
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