Oxtet_00180 Oxtet_00180   Oxtet_00200 Oxtet_00200

Oxtet_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 10970 (=NBRC 12907)
Entry nameOxytetracycline
Contig
Start / Stop / Direction19,492 / 21,003 / + [in whole cluster]
19,492 / 21,003 / + [in contig]
Location19492..21003 [in whole cluster]
19492..21003 [in contig]
TypeCDS
Length1,512 bp (503 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Productputative C-6 hydroxylase
Product (GenBank)OxyS
GeneoxyS
otcC
Gene (GenBank)oxyS
EC number
Keyword
Note
Note (GenBank)
  • oxygenase; similar to OtcC of GenBank Accession Number AAX48941
Reference
ACC
PmId
[16597959] Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase. (Appl Environ Microbiol. , 2006)
comment
oxytetracycline gene clusterのシークエンス、配列解析。
resistance genes otrAとotrBの間に全21genesを同定。
わかりやすくするため、gene namesがotc → oxyに変更され、振り直されている。

oxyS (otcC): Oxygenase

OxyE, OxyL, OxyG, and OxyS (OtcC)はそれぞれMtmOI, MtmOII, MtmOIII, and MtmOIVにとても似ているので、oxy and mtm gene clustersは進化的に近く関係する。

既にOtcCとしてanhydrotetracycline oxygenaseであると同定されている。
Related Reference
ACC
Q58PK7
PmId
[16148009] Ablation of the otcC gene encoding a post-polyketide hydroxylase from the oxytetracyline biosynthetic pathway in Streptomyces rimosus results in novel polyketides with altered chain length. (J Biol Chem. , 2005)
comment
Blast 2nd, id99%, 0.0
Streptomyces rimosus_otcC
Anhydrotetracycline oxygenase

otcC 遺伝子がpolyketide backboneの生合成後、anthracycline structureのC-6位をhydroxyl化するanhydrotetracycline oxygenase をエンコードしていること、また、otcC破壊株では新規のC-17 polyketide が生じることから、otcC遺伝子産物が無いことによりOxytetracycline (OTC)の“minimal”polyketide synthaseに影響を及ぼすことが示されている。

S. rimosus R6-500使用。

close this sectionSequence

selected fasta
>putative C-6 hydroxylase [OxyS]
MRYDVVIAGAGPTGLMLACELRLAGARTLVLERLAEPVDFSKALGVHARTVELLDMRGLG
EGFQAEAPKLRGGNFASLGVPLDFSSFDTRHPYALFVPQVRTEELLTGRALELGAELRRG
HAVTALEQDADGVTVSVTGPEGPYEVECAYLVGCDGGGSTVRKLLGIDFPGQDPHMFAVI
ADARFREELPHGEGMGPMRPYGVMRHDLRAWFAAFPLEPDVYRATVAFFDRPYADRRAPV
TEEDVRAALTEVAGSDFGMHDVRWLSRLTDTSRQAERYRDGRVLLAGDACHIHLPAGGQG
LNLGFQDAVNLGWKLGATIAGTAPPELLDTYEAERRPIAAGVLRNTRAQAVLIDPDPRYE
GLRELMIELLHVPETNRYLAGLISALDVRYPMAGEHPLLGRRVPDLPLVTEDGTRQLSTY
FHAARGVLLTLGCDQPLADEAAAWKDRVDLVAAEGVADPGSAVDGLTALLVRPDGYICWT
AAPETGTDGLTDALRTWFGPPAM
selected fasta
>putative C-6 hydroxylase [OxyS]
ATGCGGTACGACGTGGTGATCGCCGGCGCCGGGCCGACCGGCCTGATGCTCGCCTGCGAA
CTGCGCCTGGCCGGTGCCAGGACCCTGGTGCTGGAACGCCTGGCGGAGCCGGTCGACTTC
TCCAAGGCCCTCGGTGTGCACGCGCGCACCGTCGAGCTGCTGGACATGCGCGGGCTGGGC
GAGGGGTTCCAGGCCGAGGCGCCGAAGCTGCGCGGCGGGAACTTCGCCAGCCTCGGAGTA
CCCCTGGACTTCTCGTCGTTCGACACCCGGCACCCGTACGCGCTGTTCGTCCCCCAGGTG
CGTACCGAGGAGCTGCTGACCGGGCGGGCCCTGGAGCTGGGGGCCGAGCTGCGGCGCGGG
CACGCGGTGACGGCGCTGGAGCAGGACGCCGACGGCGTGACCGTCTCCGTCACCGGGCCC
GAGGGGCCGTACGAGGTCGAGTGCGCGTACCTGGTGGGCTGCGACGGCGGCGGCAGCACC
GTACGCAAGCTGCTCGGCATCGACTTCCCCGGGCAGGACCCGCACATGTTCGCGGTGATC
GCGGATGCCCGGTTCCGTGAGGAGCTGCCGCACGGGGAGGGCATGGGACCGATGCGGCCG
TACGGCGTCATGCGGCACGACCTGCGCGCCTGGTTCGCCGCCTTCCCGCTGGAGCCGGAC
GTCTACCGGGCCACGGTCGCCTTCTTCGACCGGCCCTACGCCGACCGCCGGGCGCCGGTC
ACCGAGGAGGACGTGCGGGCCGCGCTGACCGAGGTGGCCGGCAGCGACTTCGGGATGCAC
GACGTGCGCTGGCTCTCCCGGCTCACCGACACCTCACGCCAGGCCGAGCGGTACCGCGAC
GGCCGGGTCCTGCTGGCCGGCGACGCCTGCCACATCCACCTGCCCGCGGGCGGCCAGGGC
CTCAACCTGGGCTTCCAGGACGCGGTGAACCTGGGCTGGAAGCTGGGCGCGACCATCGCG
GGCACGGCTCCGCCGGAGCTGCTGGACACCTACGAGGCCGAGCGCCGCCCGATCGCCGCC
GGCGTACTGCGCAACACCCGCGCGCAAGCCGTGCTGATCGACCCGGACCCGCGTTACGAG
GGGCTGCGGGAGCTGATGATCGAGCTGCTGCACGTCCCGGAGACCAACCGCTATCTGGCG
GGCCTCATCTCGGCGCTGGACGTGCGGTACCCCATGGCGGGCGAGCACCCGCTGCTGGGG
CGCCGCGTACCGGACCTGCCGCTCGTCACCGAGGACGGCACCCGGCAGCTGAGCACGTAC
TTCCACGCGGCGCGCGGCGTCCTGCTGACGCTCGGCTGCGACCAGCCGCTCGCCGACGAG
GCCGCGGCCTGGAAGGACCGGGTGGACCTGGTCGCGGCCGAGGGCGTCGCGGACCCGGGC
TCCGCGGTCGACGGGCTGACCGCGCTGCTCGTACGCCCCGACGGCTACATCTGCTGGACC
GCGGCTCCGGAGACGGGTACGGACGGGCTCACCGACGCCCTGCGCACCTGGTTCGGGCCG
CCCGCCATGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
[3-345] 3.90000000000001e-89 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
[4-26] 3.29999077503323e-43 PR00420 [147-162] 3.29999077503323e-43 PR00420 [280-295] 3.29999077503323e-43 PR00420 [295-311] 3.29999077503323e-43 PR00420 [313-331] 3.29999077503323e-43 PR00420 [331-347] 3.29999077503323e-43 PR00420
PR00420   RNGMNOXGNASE
SignalP No significant hit
TMHMM No significant hit
Oxtet_00180 Oxtet_00180   Oxtet_00200 Oxtet_00200
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