Madu_00010 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	6,459 / 7,793 / + [in whole cluster] 6,459 / 7,793 / + [in contig]
Location	6459..7793 [in whole cluster] 6459..7793 [in contig]
Type	CDS
Length	1,335 bp (444 aa)

Annotation

Category	5.1 general function
Product	putative oxygenase
Product (GenBank)	dioxygenase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in maduropeptin biosynthesis.
Note (GenBank)	mdp orf-5
Reference	ACC B0BLL0 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 orf(-5): Dioxygenase Maduropeptin生合成遺伝子クラスターは、遺伝子産物の推定機能よりmdpA1からmdpE2までとしている。このORFについて機能解析はしていない。
Related Reference	ACC Q6E4P3 PmId [15516502] Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowers. (Plant Physiol. , 2004) comment Petunia hybrida_CCD1 Carotenoid cleavage dioxygenase 1 CCD1はbeta-caloteinを酸化的切断する酵素。beta-iononeとC14 dialdehydeを形成する。 PhCCD1 activityとbeta-ionone emissionは、転写レベルで制御されていた。
Related Reference	ACC A5HV13 PmId [17516050] Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27. (Arch Microbiol. , 2007) comment Pseudomonas putida_iso Isoeugenol monooxygenase [30%id, 2e-40] ligninに関連するフェニルプロパノイドのisoeugenol分解酵素についての報告。酸素原子ひとつを取り込んで分解することが確認されている。 dioxygenaseと配列は似ているがmonooxygenaseであるとしている。なお、真核のcarotenoid dioxygenaseも2002年にdioxygenaseではなくmonooxygenaseであることが実験で明らかとなった、との記述あり。

Sequence

>putative oxygenase [dioxygenase]
MTQDTGIWLEGHLAPVPDEIDAPDLPVTGTLPPELTGRYFRNGPNPRPGRPSTHWFTGAG
MIHGVRLRAGRAEWYRNRWVRTRELTEDAPFLRDDGTLDLTAVPANTHVVRHAGKILALV
EAGLPHALTPELGTVGLEDFGGRLTTAMTAHPKRDPVTGELLFFGYGVFPPHLTYHRLSA
DGRLLESREIDVPAGTMTHDFAITENHVVWLDLPVVFDLDLAMKETSMPFRWDDAYGARL
GVMPREGGGDVRWFDIDPCYVFHVGNAHEDGAGRIVLDAVRYAPDEFTALWAMIGGSADP
AREAGLLEEARLHRWTLDPATGRAAEEPLDDRGVEFPTHDDDRTGLPNRFLYTVSGNAIV
KYDVRDGSSSAREFEEGASVGEAVFVPAEGARAEDEGWLLSIVSGPSGADLVVMDATDVS
RTTASVRLPRRVPAGFHGSWIPDS

>putative oxygenase [dioxygenase]
ATGACGCAGGACACCGGCATATGGCTTGAAGGACACCTCGCCCCCGTCCCGGACGAGATC
GACGCGCCCGACCTTCCCGTCACCGGCACGCTCCCGCCCGAGCTGACCGGGCGCTACTTC
CGCAACGGCCCCAACCCGCGGCCGGGACGGCCGAGCACCCACTGGTTCACCGGCGCCGGG
ATGATCCACGGCGTGCGGCTCCGCGCGGGCCGGGCCGAGTGGTACCGCAACCGCTGGGTC
CGGACCCGGGAGCTCACCGAGGACGCCCCGTTCCTGCGGGACGACGGGACGCTCGACCTC
ACCGCGGTGCCCGCCAACACCCACGTCGTCCGGCACGCGGGGAAGATCCTCGCGCTCGTC
GAGGCCGGCCTCCCGCACGCGCTGACGCCCGAGCTCGGGACGGTCGGCCTCGAGGACTTC
GGCGGGCGCCTCACCACCGCGATGACCGCGCACCCGAAGCGGGATCCGGTGACGGGCGAG
CTGCTGTTCTTCGGCTACGGCGTCTTCCCGCCCCACCTGACCTACCACCGGCTCTCGGCG
GACGGACGGCTCTTGGAGAGCCGCGAGATCGACGTCCCCGCCGGGACGATGACGCACGAC
TTCGCGATCACCGAGAACCACGTCGTCTGGCTCGACCTGCCCGTCGTCTTCGACCTCGAC
CTGGCGATGAAGGAGACGTCCATGCCGTTCCGCTGGGACGACGCGTACGGGGCGCGGCTC
GGCGTCATGCCCCGGGAGGGTGGCGGGGACGTCCGCTGGTTCGACATCGACCCGTGCTAC
GTGTTCCACGTCGGGAACGCCCACGAGGACGGGGCGGGCCGCATCGTCCTCGACGCCGTG
CGCTACGCCCCCGACGAGTTCACCGCCCTCTGGGCGATGATCGGCGGGTCGGCCGACCCC
GCCCGGGAGGCCGGCCTCCTGGAGGAGGCCCGCCTGCACCGCTGGACGCTCGACCCCGCG
ACCGGACGCGCGGCCGAGGAGCCGCTGGACGACCGGGGCGTGGAGTTCCCCACCCACGAC
GACGACCGGACGGGCCTGCCGAACCGGTTCCTCTACACCGTGTCCGGGAACGCGATCGTG
AAGTACGACGTGCGCGACGGGTCCTCCTCCGCGCGCGAGTTCGAGGAGGGCGCGAGCGTC
GGCGAGGCCGTCTTCGTCCCGGCCGAGGGGGCGCGTGCGGAGGACGAGGGCTGGCTGCTG
TCGATCGTCTCCGGCCCGTCCGGCGCGGATCTCGTGGTCATGGACGCGACGGACGTCTCC
CGGACCACGGCGTCCGTCCGGCTGCCCCGGCGCGTCCCGGCCGGTTTCCACGGAAGCTGG
ATCCCCGACTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004294 Carotenoid oxygenase (Family) PF03055 RPE65
SignalP	No significant hit
TMHMM	No significant hit

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