Madu_00020 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	9,065 / 7,854 / - [in whole cluster] 9,065 / 7,854 / - [in contig]
Location	complement(7854..9065) [in whole cluster] complement(7854..9065) [in contig]
Type	CDS
Length	1,212 bp (403 aa)

Annotation

Category	5.1 general function
Product	putative oxygenase
Product (GenBank)	dioxygenase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in maduropeptin biosynthesis.
Note (GenBank)	mdp orf-4
Reference	ACC B0BLL1 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 orf(-4): Dioxygenase Maduropeptin生合成遺伝子クラスターは、遺伝子産物の推定機能よりmdpA1からmdpE2までとしている。このORFについて機能解析はしていない。
Related Reference	ACC A5HV13 PmId [17516050] Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27. (Arch Microbiol. , 2007) comment Pseudomonas putida_iso Isoeugenol monooxygenase [26%id, 7e-12] ligninに関連するフェニルプロパノイドのisoeugenol分解酵素についての報告。酸素原子ひとつを取り込んで分解することが確認されている。 dioxygenaseと配列は似ているがmonooxygenaseであるとしている。なお、真核のcarotenoid dioxygenaseも2002年にdioxygenaseではなくmonooxygenaseであることが実験で明らかとなった、との記述あり。

Sequence

>putative oxygenase [dioxygenase]
MMAAETQGTDPRTAPAAAPAHRADAPLPVAGGLPPGLRACFLQAETHPLAPGAGGEILAG
PHLLSGIVLDGGTAWWLRCAPPVGRNRPLGPVPALAPVLWTGAREADPDGPGTVAFARPV
RDPASGRWHTVATYPGLGHAEHLVTAPDGTVVHAEPFPLDGAPLVHAVGLTARYLVVFDL
PVAHSRAAALVGERPPYAWREGRPARVGFLPRAGGAPVTWVPVDPCYVFDVVDARDDGPH
VVLDVVAARRAFDGAGASPSRLRRWRFDPAAGTAAPLRTSAPVTGAVAVPAAEPGAHRLV
ATEPGGTVVLHDPALGTSARTSLGRGWRPGAPVFAPGPETGGWILAVARHAASGRCELRV
LDTSGRPVAAVRLPVRLAAGRAVRSAAVPDAGALAGFAARAAT

>putative oxygenase [dioxygenase]
GTGATGGCCGCCGAGACGCAGGGAACCGATCCGCGGACGGCGCCGGCCGCCGCGCCCGCC
CACCGCGCGGACGCTCCGCTCCCCGTGGCGGGCGGGCTCCCCCCGGGCCTGCGCGCCTGC
TTCCTCCAGGCCGAGACGCACCCGCTGGCGCCCGGCGCGGGCGGCGAGATCCTCGCCGGG
CCGCACCTGCTCTCCGGCATCGTGCTCGACGGCGGGACCGCGTGGTGGCTCCGCTGCGCG
CCGCCCGTCGGGCGGAACCGGCCGCTCGGCCCCGTCCCGGCGCTCGCGCCCGTCCTGTGG
ACGGGCGCGCGGGAGGCGGATCCGGACGGGCCGGGGACGGTCGCGTTCGCGCGTCCGGTC
CGGGACCCGGCGAGCGGCCGGTGGCACACCGTCGCGACCTATCCGGGCCTCGGGCACGCC
GAACACCTGGTCACCGCGCCGGACGGCACCGTCGTCCACGCCGAGCCGTTCCCGCTCGAC
GGCGCTCCGCTCGTCCACGCCGTCGGCCTCACCGCCCGGTACCTCGTCGTGTTCGACCTG
CCCGTGGCCCACTCGCGCGCCGCCGCGCTGGTCGGGGAGCGGCCGCCGTACGCGTGGCGG
GAGGGCCGGCCCGCGCGGGTGGGGTTCCTGCCCCGCGCCGGAGGGGCGCCCGTGACCTGG
GTCCCCGTCGACCCCTGCTACGTCTTCGACGTGGTCGACGCCCGCGACGACGGACCGCAC
GTCGTGCTGGACGTCGTCGCGGCGCGGCGCGCGTTCGACGGAGCGGGCGCGTCCCCGTCC
CGGCTGCGGCGGTGGCGCTTCGACCCCGCCGCCGGGACGGCCGCGCCCCTCCGCACGTCC
GCCCCGGTCACCGGCGCGGTCGCCGTCCCGGCCGCGGAACCGGGCGCGCACCGCCTGGTC
GCGACCGAGCCCGGCGGGACGGTCGTCCTGCACGACCCGGCCCTCGGCACGTCCGCCCGC
ACGTCGCTCGGCCGGGGGTGGCGGCCCGGCGCGCCCGTCTTCGCGCCCGGCCCGGAGACC
GGGGGCTGGATCCTCGCCGTGGCGCGGCACGCCGCGTCCGGACGCTGCGAGCTGCGCGTC
CTCGACACCTCCGGCCGTCCGGTGGCGGCGGTGCGGCTGCCCGTGCGGCTCGCGGCCGGC
CGGGCCGTCCGGTCGGCCGCCGTCCCCGACGCCGGCGCCCTCGCCGGCTTCGCCGCCCGC
GCCGCCACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004294 Carotenoid oxygenase (Family) PF03055 RPE65
SignalP	No significant hit
TMHMM	No significant hit

Madu_00010 Madu_00030